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Meiotic nuclear divisions in budding yeast require PP2A(Cdc55)-mediated antagonism of Net1 phosphorylation by Cdk

During meiosis, one round of deoxyribonucleic acid replication is followed by two rounds of nuclear division. In Saccharomyces cerevisiae, activation of the Cdc14 early anaphase release (FEAR) network is required for exit from meiosis I but does not lead to the activation of origins of replication....

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Autores principales: Kerr, Gary W., Sarkar, Sourav, Tibbles, Katherine L., Petronczki, Mark, Millar, Jonathan B.A., Arumugam, Prakash
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216327/
https://www.ncbi.nlm.nih.gov/pubmed/21690311
http://dx.doi.org/10.1083/jcb.201103019
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author Kerr, Gary W.
Sarkar, Sourav
Tibbles, Katherine L.
Petronczki, Mark
Millar, Jonathan B.A.
Arumugam, Prakash
author_facet Kerr, Gary W.
Sarkar, Sourav
Tibbles, Katherine L.
Petronczki, Mark
Millar, Jonathan B.A.
Arumugam, Prakash
author_sort Kerr, Gary W.
collection PubMed
description During meiosis, one round of deoxyribonucleic acid replication is followed by two rounds of nuclear division. In Saccharomyces cerevisiae, activation of the Cdc14 early anaphase release (FEAR) network is required for exit from meiosis I but does not lead to the activation of origins of replication. The precise mechanism of how FEAR regulates meiosis is not understood. In this paper, we report that premature activation of FEAR during meiosis caused by loss of protein phosphatase PP2A(Cdc55) activity blocks bipolar spindle assembly and nuclear divisions. In cdc55 meiotic null (cdc55-mn) cells, the cyclin-dependent kinase (Cdk)–counteracting phosphatase Cdc14 was released prematurely from the nucleolus concomitant with hyperphosphorylation of its nucleolar anchor protein Net1. Crucially, a mutant form of Net1 that lacks six Cdk phosphorylation sites rescued the meiotic defect of cdc55-mn cells. Expression of a dominant mutant allele of CDC14 mimicked the cdc55-mn phenotype. We propose that phosphoregulation of Net1 by PP2A(Cdc55) is essential for preventing precocious exit from meiosis I.
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spelling pubmed-32163272011-12-27 Meiotic nuclear divisions in budding yeast require PP2A(Cdc55)-mediated antagonism of Net1 phosphorylation by Cdk Kerr, Gary W. Sarkar, Sourav Tibbles, Katherine L. Petronczki, Mark Millar, Jonathan B.A. Arumugam, Prakash J Cell Biol Research Articles During meiosis, one round of deoxyribonucleic acid replication is followed by two rounds of nuclear division. In Saccharomyces cerevisiae, activation of the Cdc14 early anaphase release (FEAR) network is required for exit from meiosis I but does not lead to the activation of origins of replication. The precise mechanism of how FEAR regulates meiosis is not understood. In this paper, we report that premature activation of FEAR during meiosis caused by loss of protein phosphatase PP2A(Cdc55) activity blocks bipolar spindle assembly and nuclear divisions. In cdc55 meiotic null (cdc55-mn) cells, the cyclin-dependent kinase (Cdk)–counteracting phosphatase Cdc14 was released prematurely from the nucleolus concomitant with hyperphosphorylation of its nucleolar anchor protein Net1. Crucially, a mutant form of Net1 that lacks six Cdk phosphorylation sites rescued the meiotic defect of cdc55-mn cells. Expression of a dominant mutant allele of CDC14 mimicked the cdc55-mn phenotype. We propose that phosphoregulation of Net1 by PP2A(Cdc55) is essential for preventing precocious exit from meiosis I. The Rockefeller University Press 2011-06-27 /pmc/articles/PMC3216327/ /pubmed/21690311 http://dx.doi.org/10.1083/jcb.201103019 Text en © 2011 Kerr et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Kerr, Gary W.
Sarkar, Sourav
Tibbles, Katherine L.
Petronczki, Mark
Millar, Jonathan B.A.
Arumugam, Prakash
Meiotic nuclear divisions in budding yeast require PP2A(Cdc55)-mediated antagonism of Net1 phosphorylation by Cdk
title Meiotic nuclear divisions in budding yeast require PP2A(Cdc55)-mediated antagonism of Net1 phosphorylation by Cdk
title_full Meiotic nuclear divisions in budding yeast require PP2A(Cdc55)-mediated antagonism of Net1 phosphorylation by Cdk
title_fullStr Meiotic nuclear divisions in budding yeast require PP2A(Cdc55)-mediated antagonism of Net1 phosphorylation by Cdk
title_full_unstemmed Meiotic nuclear divisions in budding yeast require PP2A(Cdc55)-mediated antagonism of Net1 phosphorylation by Cdk
title_short Meiotic nuclear divisions in budding yeast require PP2A(Cdc55)-mediated antagonism of Net1 phosphorylation by Cdk
title_sort meiotic nuclear divisions in budding yeast require pp2a(cdc55)-mediated antagonism of net1 phosphorylation by cdk
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216327/
https://www.ncbi.nlm.nih.gov/pubmed/21690311
http://dx.doi.org/10.1083/jcb.201103019
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