Epitope Analysis of Cerebrospinal Fluid IgG in Japanese Multiple Sclerosis Patients Using Phage Display Method

To investigate the antigen recognized by cerebrospinal fluid (CSF) high affinity IgG in patients with multiple sclerosis (MS), the phage display method was applied to the CSF from 15 MS and 10 control patients. Peptide sequences recognized by MS and control CSF IgG were individual specific, and no c...

Descripción completa

Detalles Bibliográficos
Autores principales: Fujimori, Juichi, Nakashima, Ichiro, Fujihara, Kazuo, Misu, Tatsuro, Sato, Shigeru, Itoyama, Yasuto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216389/
https://www.ncbi.nlm.nih.gov/pubmed/22132333
http://dx.doi.org/10.1155/2011/353417
_version_ 1782216509923262464
author Fujimori, Juichi
Nakashima, Ichiro
Fujihara, Kazuo
Misu, Tatsuro
Sato, Shigeru
Itoyama, Yasuto
author_facet Fujimori, Juichi
Nakashima, Ichiro
Fujihara, Kazuo
Misu, Tatsuro
Sato, Shigeru
Itoyama, Yasuto
author_sort Fujimori, Juichi
collection PubMed
description To investigate the antigen recognized by cerebrospinal fluid (CSF) high affinity IgG in patients with multiple sclerosis (MS), the phage display method was applied to the CSF from 15 MS and 10 control patients. Peptide sequences recognized by MS and control CSF IgG were individual specific, and no common motif was found. Peptide sequences frequently showed homology to various kinds of amino acid sequences of ubiquitous viruses such as epstein barr virus (EBV) and herpes simplex virus (HSV), although the frequency was not specific to MS patients. MS CSF IgG may recognize various types of ubiquitous viral antigen and may be increased by a bystander response.
format Online
Article
Text
id pubmed-3216389
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-32163892011-11-30 Epitope Analysis of Cerebrospinal Fluid IgG in Japanese Multiple Sclerosis Patients Using Phage Display Method Fujimori, Juichi Nakashima, Ichiro Fujihara, Kazuo Misu, Tatsuro Sato, Shigeru Itoyama, Yasuto Mult Scler Int Research Article To investigate the antigen recognized by cerebrospinal fluid (CSF) high affinity IgG in patients with multiple sclerosis (MS), the phage display method was applied to the CSF from 15 MS and 10 control patients. Peptide sequences recognized by MS and control CSF IgG were individual specific, and no common motif was found. Peptide sequences frequently showed homology to various kinds of amino acid sequences of ubiquitous viruses such as epstein barr virus (EBV) and herpes simplex virus (HSV), although the frequency was not specific to MS patients. MS CSF IgG may recognize various types of ubiquitous viral antigen and may be increased by a bystander response. Hindawi Publishing Corporation 2011 2011-11-10 /pmc/articles/PMC3216389/ /pubmed/22132333 http://dx.doi.org/10.1155/2011/353417 Text en Copyright © 2011 Juichi Fujimori et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Fujimori, Juichi
Nakashima, Ichiro
Fujihara, Kazuo
Misu, Tatsuro
Sato, Shigeru
Itoyama, Yasuto
Epitope Analysis of Cerebrospinal Fluid IgG in Japanese Multiple Sclerosis Patients Using Phage Display Method
title Epitope Analysis of Cerebrospinal Fluid IgG in Japanese Multiple Sclerosis Patients Using Phage Display Method
title_full Epitope Analysis of Cerebrospinal Fluid IgG in Japanese Multiple Sclerosis Patients Using Phage Display Method
title_fullStr Epitope Analysis of Cerebrospinal Fluid IgG in Japanese Multiple Sclerosis Patients Using Phage Display Method
title_full_unstemmed Epitope Analysis of Cerebrospinal Fluid IgG in Japanese Multiple Sclerosis Patients Using Phage Display Method
title_short Epitope Analysis of Cerebrospinal Fluid IgG in Japanese Multiple Sclerosis Patients Using Phage Display Method
title_sort epitope analysis of cerebrospinal fluid igg in japanese multiple sclerosis patients using phage display method
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216389/
https://www.ncbi.nlm.nih.gov/pubmed/22132333
http://dx.doi.org/10.1155/2011/353417
work_keys_str_mv AT fujimorijuichi epitopeanalysisofcerebrospinalfluidigginjapanesemultiplesclerosispatientsusingphagedisplaymethod
AT nakashimaichiro epitopeanalysisofcerebrospinalfluidigginjapanesemultiplesclerosispatientsusingphagedisplaymethod
AT fujiharakazuo epitopeanalysisofcerebrospinalfluidigginjapanesemultiplesclerosispatientsusingphagedisplaymethod
AT misutatsuro epitopeanalysisofcerebrospinalfluidigginjapanesemultiplesclerosispatientsusingphagedisplaymethod
AT satoshigeru epitopeanalysisofcerebrospinalfluidigginjapanesemultiplesclerosispatientsusingphagedisplaymethod
AT itoyamayasuto epitopeanalysisofcerebrospinalfluidigginjapanesemultiplesclerosispatientsusingphagedisplaymethod

Ejemplares similares