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Time-course analysis of the Shewanella amazonensis SB2B proteome in response to sodium chloride shock
Shewanellae are microbial models for environmental stress response; however, the sequential expression of mechanisms in response to stress is poorly understood. Here we experimentally determine the response mechanisms of Shewanella amazonensis SB2B during sodium chloride stress using a novel liquid...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216512/ https://www.ncbi.nlm.nih.gov/pubmed/22355544 http://dx.doi.org/10.1038/srep00025 |
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author | Parnell, J. Jacob Callister, Stephen J. Rompato, Giovanni Nicora, Carrie D. Paša-Tolić, Ljiljana Williamson, Ashley Pfrender, Michael E. |
author_facet | Parnell, J. Jacob Callister, Stephen J. Rompato, Giovanni Nicora, Carrie D. Paša-Tolić, Ljiljana Williamson, Ashley Pfrender, Michael E. |
author_sort | Parnell, J. Jacob |
collection | PubMed |
description | Shewanellae are microbial models for environmental stress response; however, the sequential expression of mechanisms in response to stress is poorly understood. Here we experimentally determine the response mechanisms of Shewanella amazonensis SB2B during sodium chloride stress using a novel liquid chromatography and accurate mass-time tag mass spectrometry time-course proteomics approach. The response of SB2B involves an orchestrated sequence of events comprising increased signal transduction associated with motility and restricted growth. Following a metabolic shift to branched chain amino acid degradation, motility and cellular replication proteins return to pre-perturbed levels. Although sodium chloride stress is associated with a change in the membrane fatty acid composition in other organisms, this is not the case for SB2B as fatty acid degradation pathways are not expressed and no change in the fatty acid profile is observed. These findings suggest that shifts in membrane composition may be an indirect physiological response to high NaCl stress. |
format | Online Article Text |
id | pubmed-3216512 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-32165122011-12-22 Time-course analysis of the Shewanella amazonensis SB2B proteome in response to sodium chloride shock Parnell, J. Jacob Callister, Stephen J. Rompato, Giovanni Nicora, Carrie D. Paša-Tolić, Ljiljana Williamson, Ashley Pfrender, Michael E. Sci Rep Article Shewanellae are microbial models for environmental stress response; however, the sequential expression of mechanisms in response to stress is poorly understood. Here we experimentally determine the response mechanisms of Shewanella amazonensis SB2B during sodium chloride stress using a novel liquid chromatography and accurate mass-time tag mass spectrometry time-course proteomics approach. The response of SB2B involves an orchestrated sequence of events comprising increased signal transduction associated with motility and restricted growth. Following a metabolic shift to branched chain amino acid degradation, motility and cellular replication proteins return to pre-perturbed levels. Although sodium chloride stress is associated with a change in the membrane fatty acid composition in other organisms, this is not the case for SB2B as fatty acid degradation pathways are not expressed and no change in the fatty acid profile is observed. These findings suggest that shifts in membrane composition may be an indirect physiological response to high NaCl stress. Nature Publishing Group 2011-06-29 /pmc/articles/PMC3216512/ /pubmed/22355544 http://dx.doi.org/10.1038/srep00025 Text en Copyright © 2011, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Parnell, J. Jacob Callister, Stephen J. Rompato, Giovanni Nicora, Carrie D. Paša-Tolić, Ljiljana Williamson, Ashley Pfrender, Michael E. Time-course analysis of the Shewanella amazonensis SB2B proteome in response to sodium chloride shock |
title | Time-course analysis of the Shewanella amazonensis SB2B proteome in response to sodium chloride shock |
title_full | Time-course analysis of the Shewanella amazonensis SB2B proteome in response to sodium chloride shock |
title_fullStr | Time-course analysis of the Shewanella amazonensis SB2B proteome in response to sodium chloride shock |
title_full_unstemmed | Time-course analysis of the Shewanella amazonensis SB2B proteome in response to sodium chloride shock |
title_short | Time-course analysis of the Shewanella amazonensis SB2B proteome in response to sodium chloride shock |
title_sort | time-course analysis of the shewanella amazonensis sb2b proteome in response to sodium chloride shock |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216512/ https://www.ncbi.nlm.nih.gov/pubmed/22355544 http://dx.doi.org/10.1038/srep00025 |
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