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Non-specific binding of antibodies in immunohistochemistry: fallacies and facts
The current protocols for blocking background staining in immunohistochemistry are based on conflicting reports. Background staining is thought to occur as a result of either non-specific antibody (Ab) binding to endogenous Fc receptors (FcRs) or a combination of ionic and hydrophobic interactions....
Autores principales: | Buchwalow, Igor, Samoilova, Vera, Boecker, Werner, Tiemann, Markus |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216515/ https://www.ncbi.nlm.nih.gov/pubmed/22355547 http://dx.doi.org/10.1038/srep00028 |
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