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Commercial Teas Highlight Plant DNA Barcode Identification Successes and Obstacles

Appearance does not easily identify the dried plant fragments used to prepare teas to species. Here we test recovery of standard DNA barcodes for land plants from a large array of commercial tea products and analyze their performance in identifying tea constituents using existing databases. Most (90...

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Autores principales: Stoeckle, Mark Y., Gamble, Catherine C., Kirpekar, Rohan, Young, Grace, Ahmed, Selena, Little, Damon P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216529/
https://www.ncbi.nlm.nih.gov/pubmed/22355561
http://dx.doi.org/10.1038/srep00042
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author Stoeckle, Mark Y.
Gamble, Catherine C.
Kirpekar, Rohan
Young, Grace
Ahmed, Selena
Little, Damon P.
author_facet Stoeckle, Mark Y.
Gamble, Catherine C.
Kirpekar, Rohan
Young, Grace
Ahmed, Selena
Little, Damon P.
author_sort Stoeckle, Mark Y.
collection PubMed
description Appearance does not easily identify the dried plant fragments used to prepare teas to species. Here we test recovery of standard DNA barcodes for land plants from a large array of commercial tea products and analyze their performance in identifying tea constituents using existing databases. Most (90%) of 146 tea products yielded rbcL or matK barcodes using a standard protocol. Matching DNA identifications to listed ingredients was limited by incomplete databases for the two markers, shared or nearly identical barcodes among some species, and lack of standard common names for plant species. About 1/3 of herbal teas generated DNA identifications not found on labels. Broad scale adoption of plant DNA barcoding may require algorithms that place search results in context of standard plant names and character-based keys for distinguishing closely-related species. Demonstrating the importance of accessible plant barcoding, our findings indicate unlisted ingredients are common in herbal teas.
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spelling pubmed-32165292011-12-22 Commercial Teas Highlight Plant DNA Barcode Identification Successes and Obstacles Stoeckle, Mark Y. Gamble, Catherine C. Kirpekar, Rohan Young, Grace Ahmed, Selena Little, Damon P. Sci Rep Article Appearance does not easily identify the dried plant fragments used to prepare teas to species. Here we test recovery of standard DNA barcodes for land plants from a large array of commercial tea products and analyze their performance in identifying tea constituents using existing databases. Most (90%) of 146 tea products yielded rbcL or matK barcodes using a standard protocol. Matching DNA identifications to listed ingredients was limited by incomplete databases for the two markers, shared or nearly identical barcodes among some species, and lack of standard common names for plant species. About 1/3 of herbal teas generated DNA identifications not found on labels. Broad scale adoption of plant DNA barcoding may require algorithms that place search results in context of standard plant names and character-based keys for distinguishing closely-related species. Demonstrating the importance of accessible plant barcoding, our findings indicate unlisted ingredients are common in herbal teas. Nature Publishing Group 2011-07-21 /pmc/articles/PMC3216529/ /pubmed/22355561 http://dx.doi.org/10.1038/srep00042 Text en Copyright © 2011, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Article
Stoeckle, Mark Y.
Gamble, Catherine C.
Kirpekar, Rohan
Young, Grace
Ahmed, Selena
Little, Damon P.
Commercial Teas Highlight Plant DNA Barcode Identification Successes and Obstacles
title Commercial Teas Highlight Plant DNA Barcode Identification Successes and Obstacles
title_full Commercial Teas Highlight Plant DNA Barcode Identification Successes and Obstacles
title_fullStr Commercial Teas Highlight Plant DNA Barcode Identification Successes and Obstacles
title_full_unstemmed Commercial Teas Highlight Plant DNA Barcode Identification Successes and Obstacles
title_short Commercial Teas Highlight Plant DNA Barcode Identification Successes and Obstacles
title_sort commercial teas highlight plant dna barcode identification successes and obstacles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216529/
https://www.ncbi.nlm.nih.gov/pubmed/22355561
http://dx.doi.org/10.1038/srep00042
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