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Cross-Reactivity and Expansion of Dengue-Specific T cells During Acute Primary and Secondary Infections in Humans

Serotype-cross-reactive memory T cells responding to secondary dengue virus (DENV) infection are thought to contribute to disease. However, epitope-specific T cell responses have not been thoroughly compared between subjects with primary versus secondary DENV infection. We studied CD8(+) T cells spe...

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Autores principales: Friberg, Heather, Bashyam, Hema, Toyosaki-Maeda, Tomoko, Potts, James A., Greenough, Thomas, Kalayanarooj, Siripen, Gibbons, Robert V., Nisalak, Ananda, Srikiatkhachorn, Anon, Green, Sharone, Stephens, Henry A. F., Rothman, Alan L., Mathew, Anuja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216538/
https://www.ncbi.nlm.nih.gov/pubmed/22355570
http://dx.doi.org/10.1038/srep00051
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author Friberg, Heather
Bashyam, Hema
Toyosaki-Maeda, Tomoko
Potts, James A.
Greenough, Thomas
Kalayanarooj, Siripen
Gibbons, Robert V.
Nisalak, Ananda
Srikiatkhachorn, Anon
Green, Sharone
Stephens, Henry A. F.
Rothman, Alan L.
Mathew, Anuja
author_facet Friberg, Heather
Bashyam, Hema
Toyosaki-Maeda, Tomoko
Potts, James A.
Greenough, Thomas
Kalayanarooj, Siripen
Gibbons, Robert V.
Nisalak, Ananda
Srikiatkhachorn, Anon
Green, Sharone
Stephens, Henry A. F.
Rothman, Alan L.
Mathew, Anuja
author_sort Friberg, Heather
collection PubMed
description Serotype-cross-reactive memory T cells responding to secondary dengue virus (DENV) infection are thought to contribute to disease. However, epitope-specific T cell responses have not been thoroughly compared between subjects with primary versus secondary DENV infection. We studied CD8(+) T cells specific for the HLA-A*1101-restricted NS3(133) epitope in a cohort of A11(+) DENV-infected patients throughout acute illness and convalescence. We compared the expansion, serotype-cross-reactivity, and activation of these cells in PBMC from patients experiencing primary or secondary infection and mild or severe disease by flow cytometry. Our results show expansion and activation of DENV-specific CD8(+) T cells during acute infection, which are predominantly serotype-cross-reactive regardless of DENV infection history. These data confirm marked T cell activation and serotype-cross-reactivity during the febrile phase of dengue; however, A11-NS3(133)-specific responses did not correlate with prior antigenic exposure or current disease severity.
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spelling pubmed-32165382011-12-22 Cross-Reactivity and Expansion of Dengue-Specific T cells During Acute Primary and Secondary Infections in Humans Friberg, Heather Bashyam, Hema Toyosaki-Maeda, Tomoko Potts, James A. Greenough, Thomas Kalayanarooj, Siripen Gibbons, Robert V. Nisalak, Ananda Srikiatkhachorn, Anon Green, Sharone Stephens, Henry A. F. Rothman, Alan L. Mathew, Anuja Sci Rep Article Serotype-cross-reactive memory T cells responding to secondary dengue virus (DENV) infection are thought to contribute to disease. However, epitope-specific T cell responses have not been thoroughly compared between subjects with primary versus secondary DENV infection. We studied CD8(+) T cells specific for the HLA-A*1101-restricted NS3(133) epitope in a cohort of A11(+) DENV-infected patients throughout acute illness and convalescence. We compared the expansion, serotype-cross-reactivity, and activation of these cells in PBMC from patients experiencing primary or secondary infection and mild or severe disease by flow cytometry. Our results show expansion and activation of DENV-specific CD8(+) T cells during acute infection, which are predominantly serotype-cross-reactive regardless of DENV infection history. These data confirm marked T cell activation and serotype-cross-reactivity during the febrile phase of dengue; however, A11-NS3(133)-specific responses did not correlate with prior antigenic exposure or current disease severity. Nature Publishing Group 2011-08-01 /pmc/articles/PMC3216538/ /pubmed/22355570 http://dx.doi.org/10.1038/srep00051 Text en Copyright © 2011, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Friberg, Heather
Bashyam, Hema
Toyosaki-Maeda, Tomoko
Potts, James A.
Greenough, Thomas
Kalayanarooj, Siripen
Gibbons, Robert V.
Nisalak, Ananda
Srikiatkhachorn, Anon
Green, Sharone
Stephens, Henry A. F.
Rothman, Alan L.
Mathew, Anuja
Cross-Reactivity and Expansion of Dengue-Specific T cells During Acute Primary and Secondary Infections in Humans
title Cross-Reactivity and Expansion of Dengue-Specific T cells During Acute Primary and Secondary Infections in Humans
title_full Cross-Reactivity and Expansion of Dengue-Specific T cells During Acute Primary and Secondary Infections in Humans
title_fullStr Cross-Reactivity and Expansion of Dengue-Specific T cells During Acute Primary and Secondary Infections in Humans
title_full_unstemmed Cross-Reactivity and Expansion of Dengue-Specific T cells During Acute Primary and Secondary Infections in Humans
title_short Cross-Reactivity and Expansion of Dengue-Specific T cells During Acute Primary and Secondary Infections in Humans
title_sort cross-reactivity and expansion of dengue-specific t cells during acute primary and secondary infections in humans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216538/
https://www.ncbi.nlm.nih.gov/pubmed/22355570
http://dx.doi.org/10.1038/srep00051
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