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β-catenin is a molecular switch that regulates transition of cell-cell adhesion to fusion
When a sperm and an oocyte unite upon fertilization, their cell membranes adhere and fuse, but little is known about the factors regulating sperm-oocyte adhesion. Here we explored the role of β-catenin in sperm-oocyte adhesion. Biochemical analysis revealed that E-cadherin and β-catenin formed a com...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216555/ https://www.ncbi.nlm.nih.gov/pubmed/22355587 http://dx.doi.org/10.1038/srep00068 |
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author | Takezawa, Youki Yoshida, Keiichi Miyado, Kenji Sato, Masahiro Nakamura, Akihiro Kawano, Natsuko Sakakibara, Keiichi Kondo, Takahiko Harada, Yuichirou Ohnami, Naoko Kanai, Seiya Miyado, Mami Saito, Hidekazu Takahashi, Yuji Akutsu, Hidenori Umezawa, Akihiro |
author_facet | Takezawa, Youki Yoshida, Keiichi Miyado, Kenji Sato, Masahiro Nakamura, Akihiro Kawano, Natsuko Sakakibara, Keiichi Kondo, Takahiko Harada, Yuichirou Ohnami, Naoko Kanai, Seiya Miyado, Mami Saito, Hidekazu Takahashi, Yuji Akutsu, Hidenori Umezawa, Akihiro |
author_sort | Takezawa, Youki |
collection | PubMed |
description | When a sperm and an oocyte unite upon fertilization, their cell membranes adhere and fuse, but little is known about the factors regulating sperm-oocyte adhesion. Here we explored the role of β-catenin in sperm-oocyte adhesion. Biochemical analysis revealed that E-cadherin and β-catenin formed a complex in oocytes and also in sperm. Sperm-oocyte adhesion was impaired when β-catenin-deficient oocytes were inseminated with sperm. Furthermore, expression of β-catenin decreased from the sperm head and the site of an oocyte to which a sperm adheres after completion of sperm-oocyte adhesion. UBE1-41, an inhibitor of ubiquitin-activating enzyme 1, inhibited the degradation of β-catenin, and reduced the fusing ability of wild-type (but not β-catenin-deficient) oocytes. These results indicate that β-catenin is not only involved in membrane adhesion, but also in the transition to membrane fusion upon fertilization. |
format | Online Article Text |
id | pubmed-3216555 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-32165552011-12-22 β-catenin is a molecular switch that regulates transition of cell-cell adhesion to fusion Takezawa, Youki Yoshida, Keiichi Miyado, Kenji Sato, Masahiro Nakamura, Akihiro Kawano, Natsuko Sakakibara, Keiichi Kondo, Takahiko Harada, Yuichirou Ohnami, Naoko Kanai, Seiya Miyado, Mami Saito, Hidekazu Takahashi, Yuji Akutsu, Hidenori Umezawa, Akihiro Sci Rep Article When a sperm and an oocyte unite upon fertilization, their cell membranes adhere and fuse, but little is known about the factors regulating sperm-oocyte adhesion. Here we explored the role of β-catenin in sperm-oocyte adhesion. Biochemical analysis revealed that E-cadherin and β-catenin formed a complex in oocytes and also in sperm. Sperm-oocyte adhesion was impaired when β-catenin-deficient oocytes were inseminated with sperm. Furthermore, expression of β-catenin decreased from the sperm head and the site of an oocyte to which a sperm adheres after completion of sperm-oocyte adhesion. UBE1-41, an inhibitor of ubiquitin-activating enzyme 1, inhibited the degradation of β-catenin, and reduced the fusing ability of wild-type (but not β-catenin-deficient) oocytes. These results indicate that β-catenin is not only involved in membrane adhesion, but also in the transition to membrane fusion upon fertilization. Nature Publishing Group 2011-08-19 /pmc/articles/PMC3216555/ /pubmed/22355587 http://dx.doi.org/10.1038/srep00068 Text en Copyright © 2011, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Takezawa, Youki Yoshida, Keiichi Miyado, Kenji Sato, Masahiro Nakamura, Akihiro Kawano, Natsuko Sakakibara, Keiichi Kondo, Takahiko Harada, Yuichirou Ohnami, Naoko Kanai, Seiya Miyado, Mami Saito, Hidekazu Takahashi, Yuji Akutsu, Hidenori Umezawa, Akihiro β-catenin is a molecular switch that regulates transition of cell-cell adhesion to fusion |
title | β-catenin is a molecular switch that regulates transition of cell-cell adhesion to fusion |
title_full | β-catenin is a molecular switch that regulates transition of cell-cell adhesion to fusion |
title_fullStr | β-catenin is a molecular switch that regulates transition of cell-cell adhesion to fusion |
title_full_unstemmed | β-catenin is a molecular switch that regulates transition of cell-cell adhesion to fusion |
title_short | β-catenin is a molecular switch that regulates transition of cell-cell adhesion to fusion |
title_sort | β-catenin is a molecular switch that regulates transition of cell-cell adhesion to fusion |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216555/ https://www.ncbi.nlm.nih.gov/pubmed/22355587 http://dx.doi.org/10.1038/srep00068 |
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