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New Abundant Microbial Groups in Aquatic Hypersaline Environments
We describe the microbiota of two hypersaline saltern ponds, one of intermediate salinity (19%) and a NaCl saturated crystallizer pond (37%) using pyrosequencing. The analyses of these metagenomes (nearly 784 Mb) reaffirmed the vast dominance of Haloquadratum walsbyi but also revealed novel, abundan...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216616/ https://www.ncbi.nlm.nih.gov/pubmed/22355652 http://dx.doi.org/10.1038/srep00135 |
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author | Ghai, Rohit Pašić, Lejla Fernández, Ana Beatriz Martin-Cuadrado, Ana-Belen Mizuno, Carolina Megumi McMahon, Katherine D. Papke, R. Thane Stepanauskas, Ramunas Rodriguez-Brito, Beltran Rohwer, Forest Sánchez-Porro, Cristina Ventosa, Antonio Rodríguez-Valera, Francisco |
author_facet | Ghai, Rohit Pašić, Lejla Fernández, Ana Beatriz Martin-Cuadrado, Ana-Belen Mizuno, Carolina Megumi McMahon, Katherine D. Papke, R. Thane Stepanauskas, Ramunas Rodriguez-Brito, Beltran Rohwer, Forest Sánchez-Porro, Cristina Ventosa, Antonio Rodríguez-Valera, Francisco |
author_sort | Ghai, Rohit |
collection | PubMed |
description | We describe the microbiota of two hypersaline saltern ponds, one of intermediate salinity (19%) and a NaCl saturated crystallizer pond (37%) using pyrosequencing. The analyses of these metagenomes (nearly 784 Mb) reaffirmed the vast dominance of Haloquadratum walsbyi but also revealed novel, abundant and previously unsuspected microbial groups. We describe for the first time, a group of low GC Actinobacteria, related to freshwater Actinobacteria, abundant in low and intermediate salinities. Metagenomic assembly revealed three new abundant microbes: a low-GC euryarchaeon with the lowest GC content described for any euryarchaeon, a high-GC euryarchaeon and a gammaproteobacterium related to Alkalilimnicola and Nitrococcus. Multiple displacement amplification and sequencing of the genome from a single archaeal cell of the new low GC euryarchaeon suggest a photoheterotrophic and polysaccharide-degrading lifestyle and its relatedness to the recently described lineage of Nanohaloarchaea. These discoveries reveal the combined power of an unbiased metagenomic and single cell genomic approach. |
format | Online Article Text |
id | pubmed-3216616 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-32166162011-12-22 New Abundant Microbial Groups in Aquatic Hypersaline Environments Ghai, Rohit Pašić, Lejla Fernández, Ana Beatriz Martin-Cuadrado, Ana-Belen Mizuno, Carolina Megumi McMahon, Katherine D. Papke, R. Thane Stepanauskas, Ramunas Rodriguez-Brito, Beltran Rohwer, Forest Sánchez-Porro, Cristina Ventosa, Antonio Rodríguez-Valera, Francisco Sci Rep Article We describe the microbiota of two hypersaline saltern ponds, one of intermediate salinity (19%) and a NaCl saturated crystallizer pond (37%) using pyrosequencing. The analyses of these metagenomes (nearly 784 Mb) reaffirmed the vast dominance of Haloquadratum walsbyi but also revealed novel, abundant and previously unsuspected microbial groups. We describe for the first time, a group of low GC Actinobacteria, related to freshwater Actinobacteria, abundant in low and intermediate salinities. Metagenomic assembly revealed three new abundant microbes: a low-GC euryarchaeon with the lowest GC content described for any euryarchaeon, a high-GC euryarchaeon and a gammaproteobacterium related to Alkalilimnicola and Nitrococcus. Multiple displacement amplification and sequencing of the genome from a single archaeal cell of the new low GC euryarchaeon suggest a photoheterotrophic and polysaccharide-degrading lifestyle and its relatedness to the recently described lineage of Nanohaloarchaea. These discoveries reveal the combined power of an unbiased metagenomic and single cell genomic approach. Nature Publishing Group 2011-10-31 /pmc/articles/PMC3216616/ /pubmed/22355652 http://dx.doi.org/10.1038/srep00135 Text en Copyright © 2011, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ |
spellingShingle | Article Ghai, Rohit Pašić, Lejla Fernández, Ana Beatriz Martin-Cuadrado, Ana-Belen Mizuno, Carolina Megumi McMahon, Katherine D. Papke, R. Thane Stepanauskas, Ramunas Rodriguez-Brito, Beltran Rohwer, Forest Sánchez-Porro, Cristina Ventosa, Antonio Rodríguez-Valera, Francisco New Abundant Microbial Groups in Aquatic Hypersaline Environments |
title | New Abundant Microbial Groups in Aquatic Hypersaline Environments |
title_full | New Abundant Microbial Groups in Aquatic Hypersaline Environments |
title_fullStr | New Abundant Microbial Groups in Aquatic Hypersaline Environments |
title_full_unstemmed | New Abundant Microbial Groups in Aquatic Hypersaline Environments |
title_short | New Abundant Microbial Groups in Aquatic Hypersaline Environments |
title_sort | new abundant microbial groups in aquatic hypersaline environments |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216616/ https://www.ncbi.nlm.nih.gov/pubmed/22355652 http://dx.doi.org/10.1038/srep00135 |
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