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Asymmetric cortical extension shifts cleavage furrow position in Drosophila neuroblasts

The cytokinetic cleavage furrow is typically positioned symmetrically relative to the cortical cell boundaries, but it can also be asymmetric. The mechanisms that control furrow site specification have been intensively studied, but how polar cortex movements influence ultimate furrow position remain...

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Autores principales: Connell, Marisa, Cabernard, Clemens, Ricketson, Derek, Doe, Chris Q., Prehoda, Kenneth E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216648/
https://www.ncbi.nlm.nih.gov/pubmed/21937716
http://dx.doi.org/10.1091/mbc.E11-02-0173
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author Connell, Marisa
Cabernard, Clemens
Ricketson, Derek
Doe, Chris Q.
Prehoda, Kenneth E.
author_facet Connell, Marisa
Cabernard, Clemens
Ricketson, Derek
Doe, Chris Q.
Prehoda, Kenneth E.
author_sort Connell, Marisa
collection PubMed
description The cytokinetic cleavage furrow is typically positioned symmetrically relative to the cortical cell boundaries, but it can also be asymmetric. The mechanisms that control furrow site specification have been intensively studied, but how polar cortex movements influence ultimate furrow position remains poorly understood. We measured the position of the apical and the basal cortex in asymmetrically dividing Drosophila neuroblasts and observed preferential displacement of the apical cortex that becomes the larger daughter cell during anaphase, effectively shifting the cleavage furrow toward the smaller daughter cell. Asymmetric cortical extension is correlated with the presence of cortical myosin II, which is polarized in neuroblasts. Loss of myosin II asymmetry by perturbing heterotrimeric G-protein signaling results in symmetric extension and equal-sized daughter cells. We propose a model in which contraction-driven asymmetric polar extension of the neuroblast cortex during anaphase contributes to asymmetric furrow position and daughter cell size.
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spelling pubmed-32166482012-01-30 Asymmetric cortical extension shifts cleavage furrow position in Drosophila neuroblasts Connell, Marisa Cabernard, Clemens Ricketson, Derek Doe, Chris Q. Prehoda, Kenneth E. Mol Biol Cell Articles The cytokinetic cleavage furrow is typically positioned symmetrically relative to the cortical cell boundaries, but it can also be asymmetric. The mechanisms that control furrow site specification have been intensively studied, but how polar cortex movements influence ultimate furrow position remains poorly understood. We measured the position of the apical and the basal cortex in asymmetrically dividing Drosophila neuroblasts and observed preferential displacement of the apical cortex that becomes the larger daughter cell during anaphase, effectively shifting the cleavage furrow toward the smaller daughter cell. Asymmetric cortical extension is correlated with the presence of cortical myosin II, which is polarized in neuroblasts. Loss of myosin II asymmetry by perturbing heterotrimeric G-protein signaling results in symmetric extension and equal-sized daughter cells. We propose a model in which contraction-driven asymmetric polar extension of the neuroblast cortex during anaphase contributes to asymmetric furrow position and daughter cell size. The American Society for Cell Biology 2011-11-15 /pmc/articles/PMC3216648/ /pubmed/21937716 http://dx.doi.org/10.1091/mbc.E11-02-0173 Text en © 2011 Connell et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Connell, Marisa
Cabernard, Clemens
Ricketson, Derek
Doe, Chris Q.
Prehoda, Kenneth E.
Asymmetric cortical extension shifts cleavage furrow position in Drosophila neuroblasts
title Asymmetric cortical extension shifts cleavage furrow position in Drosophila neuroblasts
title_full Asymmetric cortical extension shifts cleavage furrow position in Drosophila neuroblasts
title_fullStr Asymmetric cortical extension shifts cleavage furrow position in Drosophila neuroblasts
title_full_unstemmed Asymmetric cortical extension shifts cleavage furrow position in Drosophila neuroblasts
title_short Asymmetric cortical extension shifts cleavage furrow position in Drosophila neuroblasts
title_sort asymmetric cortical extension shifts cleavage furrow position in drosophila neuroblasts
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216648/
https://www.ncbi.nlm.nih.gov/pubmed/21937716
http://dx.doi.org/10.1091/mbc.E11-02-0173
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