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PTTG1/securin modulates microtubule nucleation and cell migration

Pituitary tumor transforming gene 1 (PTTG1), also known as securin, has been implicated in many biological functions, including inhibition of sister chromatid separation, DNA repair, organ development, and regulation of the expression and secretion of angiogenic and metastatic factors. Although most...

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Autores principales: Moreno-Mateos, Miguel A., Espina, Águeda G., Torres, Belén, del Estal, María M. Gámez, Romero-Franco, Ana, Ríos, Rosa M., Pintor-Toro, José A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216656/
https://www.ncbi.nlm.nih.gov/pubmed/21937724
http://dx.doi.org/10.1091/mbc.E10-10-0838
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author Moreno-Mateos, Miguel A.
Espina, Águeda G.
Torres, Belén
del Estal, María M. Gámez
Romero-Franco, Ana
Ríos, Rosa M.
Pintor-Toro, José A.
author_facet Moreno-Mateos, Miguel A.
Espina, Águeda G.
Torres, Belén
del Estal, María M. Gámez
Romero-Franco, Ana
Ríos, Rosa M.
Pintor-Toro, José A.
author_sort Moreno-Mateos, Miguel A.
collection PubMed
description Pituitary tumor transforming gene 1 (PTTG1), also known as securin, has been implicated in many biological functions, including inhibition of sister chromatid separation, DNA repair, organ development, and regulation of the expression and secretion of angiogenic and metastatic factors. Although most of these functions of securin seem to depend on the localization of PTTG1 in the nucleus of the cell, a fraction of the protein has been also detected in the cytoplasm. Here we demonstrate that, in different cell types, a portion of cytoplasmic PTTG1 is associated with the cis face of the Golgi apparatus and that this localization depends on PTTG1 phosphorylation status. In this organelle, PTTG1 forms a complex with proteins involved in microtubule nucleation, including GM130, AKAP450, and γ-tubulin. RNA interference–mediated depletion of PTTG1 produces a delay in centrosomal and noncentrosomal microtubule nucleation. Cells lacking PTTG1 show severe defects in both cell polarization and migration in wound-healing assays. To our knowledge, this is the first study reporting the role of PTTG1 in microtubule nucleation and cell polarization, two processes directly involved in cell migration. We believe that these findings will contribute to understanding the mechanisms underlying PTTG1-mediated biological functions.
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spelling pubmed-32166562012-01-30 PTTG1/securin modulates microtubule nucleation and cell migration Moreno-Mateos, Miguel A. Espina, Águeda G. Torres, Belén del Estal, María M. Gámez Romero-Franco, Ana Ríos, Rosa M. Pintor-Toro, José A. Mol Biol Cell Articles Pituitary tumor transforming gene 1 (PTTG1), also known as securin, has been implicated in many biological functions, including inhibition of sister chromatid separation, DNA repair, organ development, and regulation of the expression and secretion of angiogenic and metastatic factors. Although most of these functions of securin seem to depend on the localization of PTTG1 in the nucleus of the cell, a fraction of the protein has been also detected in the cytoplasm. Here we demonstrate that, in different cell types, a portion of cytoplasmic PTTG1 is associated with the cis face of the Golgi apparatus and that this localization depends on PTTG1 phosphorylation status. In this organelle, PTTG1 forms a complex with proteins involved in microtubule nucleation, including GM130, AKAP450, and γ-tubulin. RNA interference–mediated depletion of PTTG1 produces a delay in centrosomal and noncentrosomal microtubule nucleation. Cells lacking PTTG1 show severe defects in both cell polarization and migration in wound-healing assays. To our knowledge, this is the first study reporting the role of PTTG1 in microtubule nucleation and cell polarization, two processes directly involved in cell migration. We believe that these findings will contribute to understanding the mechanisms underlying PTTG1-mediated biological functions. The American Society for Cell Biology 2011-11-15 /pmc/articles/PMC3216656/ /pubmed/21937724 http://dx.doi.org/10.1091/mbc.E10-10-0838 Text en © 2011 Moreno-Mateos et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Moreno-Mateos, Miguel A.
Espina, Águeda G.
Torres, Belén
del Estal, María M. Gámez
Romero-Franco, Ana
Ríos, Rosa M.
Pintor-Toro, José A.
PTTG1/securin modulates microtubule nucleation and cell migration
title PTTG1/securin modulates microtubule nucleation and cell migration
title_full PTTG1/securin modulates microtubule nucleation and cell migration
title_fullStr PTTG1/securin modulates microtubule nucleation and cell migration
title_full_unstemmed PTTG1/securin modulates microtubule nucleation and cell migration
title_short PTTG1/securin modulates microtubule nucleation and cell migration
title_sort pttg1/securin modulates microtubule nucleation and cell migration
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216656/
https://www.ncbi.nlm.nih.gov/pubmed/21937724
http://dx.doi.org/10.1091/mbc.E10-10-0838
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