Cargando…

Functional analysis of the microtubule-interacting transcriptome

RNA localization is an important mechanism for achieving precise control of posttranscriptional gene expression. Previously, we demonstrated that a subset of cellular mRNAs copurify with mitotic microtubules in egg extracts of Xenopus laevis. Due to limited genomic sequence information available for...

Descripción completa

Detalles Bibliográficos
Autores principales: Sharp, Judith A., Plant, Joshua J., Ohsumi, Toshiro K., Borowsky, Mark, Blower, Michael D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216657/
https://www.ncbi.nlm.nih.gov/pubmed/21937723
http://dx.doi.org/10.1091/mbc.E11-07-0629
_version_ 1782216551558021120
author Sharp, Judith A.
Plant, Joshua J.
Ohsumi, Toshiro K.
Borowsky, Mark
Blower, Michael D.
author_facet Sharp, Judith A.
Plant, Joshua J.
Ohsumi, Toshiro K.
Borowsky, Mark
Blower, Michael D.
author_sort Sharp, Judith A.
collection PubMed
description RNA localization is an important mechanism for achieving precise control of posttranscriptional gene expression. Previously, we demonstrated that a subset of cellular mRNAs copurify with mitotic microtubules in egg extracts of Xenopus laevis. Due to limited genomic sequence information available for X. laevis, we used RNA-seq to comprehensively identify the microtubule-interacting transcriptome of the related frog Xenopus tropicalis. We identified ∼450 mRNAs that showed significant enrichment on microtubules (MT-RNAs). In addition, we demonstrated that the MT-RNAs incenp, xrhamm, and tpx2 associate with spindle microtubules in vivo. MT-RNAs are enriched with transcripts associated with cell division, spindle formation, and chromosome function, demonstrating an overrepresentation of genes involved in mitotic regulation. To test whether uncharacterized MT-RNAs have a functional role in mitosis, we performed RNA interference and discovered that several MT-RNAs are required for normal spindle pole organization and γ-tubulin distribution. Together, these data demonstrate that microtubule association is one mechanism for compartmentalizing functionally related mRNAs within the nucleocytoplasmic space of mitotic cells and suggest that MT-RNAs are likely to contribute to spindle-localized mitotic translation.
format Online
Article
Text
id pubmed-3216657
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-32166572012-01-30 Functional analysis of the microtubule-interacting transcriptome Sharp, Judith A. Plant, Joshua J. Ohsumi, Toshiro K. Borowsky, Mark Blower, Michael D. Mol Biol Cell Articles RNA localization is an important mechanism for achieving precise control of posttranscriptional gene expression. Previously, we demonstrated that a subset of cellular mRNAs copurify with mitotic microtubules in egg extracts of Xenopus laevis. Due to limited genomic sequence information available for X. laevis, we used RNA-seq to comprehensively identify the microtubule-interacting transcriptome of the related frog Xenopus tropicalis. We identified ∼450 mRNAs that showed significant enrichment on microtubules (MT-RNAs). In addition, we demonstrated that the MT-RNAs incenp, xrhamm, and tpx2 associate with spindle microtubules in vivo. MT-RNAs are enriched with transcripts associated with cell division, spindle formation, and chromosome function, demonstrating an overrepresentation of genes involved in mitotic regulation. To test whether uncharacterized MT-RNAs have a functional role in mitosis, we performed RNA interference and discovered that several MT-RNAs are required for normal spindle pole organization and γ-tubulin distribution. Together, these data demonstrate that microtubule association is one mechanism for compartmentalizing functionally related mRNAs within the nucleocytoplasmic space of mitotic cells and suggest that MT-RNAs are likely to contribute to spindle-localized mitotic translation. The American Society for Cell Biology 2011-11-15 /pmc/articles/PMC3216657/ /pubmed/21937723 http://dx.doi.org/10.1091/mbc.E11-07-0629 Text en © 2011 Sharp et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Sharp, Judith A.
Plant, Joshua J.
Ohsumi, Toshiro K.
Borowsky, Mark
Blower, Michael D.
Functional analysis of the microtubule-interacting transcriptome
title Functional analysis of the microtubule-interacting transcriptome
title_full Functional analysis of the microtubule-interacting transcriptome
title_fullStr Functional analysis of the microtubule-interacting transcriptome
title_full_unstemmed Functional analysis of the microtubule-interacting transcriptome
title_short Functional analysis of the microtubule-interacting transcriptome
title_sort functional analysis of the microtubule-interacting transcriptome
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216657/
https://www.ncbi.nlm.nih.gov/pubmed/21937723
http://dx.doi.org/10.1091/mbc.E11-07-0629
work_keys_str_mv AT sharpjuditha functionalanalysisofthemicrotubuleinteractingtranscriptome
AT plantjoshuaj functionalanalysisofthemicrotubuleinteractingtranscriptome
AT ohsumitoshirok functionalanalysisofthemicrotubuleinteractingtranscriptome
AT borowskymark functionalanalysisofthemicrotubuleinteractingtranscriptome
AT blowermichaeld functionalanalysisofthemicrotubuleinteractingtranscriptome