Cargando…

Subunit organization in the Dam1 kinetochore complex and its ring around microtubules

All eukaryotic cells must segregate their chromosomes equally between two daughter cells at each division. This process needs to be robust, as errors in the form of loss or gain of genetic material have catastrophic effects on viability. Chromosomes are captured, aligned, and segregated to daughter...

Descripción completa

Detalles Bibliográficos
Autores principales: Ramey, Vincent H., Wong, Amanda, Fang, Jie, Howes, Stuart, Barnes, Georjana, Nogales, Eva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216659/
https://www.ncbi.nlm.nih.gov/pubmed/21965284
http://dx.doi.org/10.1091/mbc.E11-07-0659
_version_ 1782216552049803264
author Ramey, Vincent H.
Wong, Amanda
Fang, Jie
Howes, Stuart
Barnes, Georjana
Nogales, Eva
author_facet Ramey, Vincent H.
Wong, Amanda
Fang, Jie
Howes, Stuart
Barnes, Georjana
Nogales, Eva
author_sort Ramey, Vincent H.
collection PubMed
description All eukaryotic cells must segregate their chromosomes equally between two daughter cells at each division. This process needs to be robust, as errors in the form of loss or gain of genetic material have catastrophic effects on viability. Chromosomes are captured, aligned, and segregated to daughter cells via interaction with spindle microtubules mediated by the kinetochore. In Saccharomyces cerevisiae one microtubule attaches to each kinetochore, requiring extreme processivity from this single connection. The yeast Dam1 complex, an essential component of the outer kinetochore, forms rings around microtubules and in vitro recapitulates much of the functionality of a kinetochore–microtubule attachment. To understand the mechanism of the Dam1 complex at the kinetochore, we must know how it binds to microtubules, how it assembles into rings, and how assembly is regulated. We used electron microscopy to map several subunits within the structure of the Dam1 complex and identify the organization of Dam1 complexes within the ring. Of importance, new data strongly support a more passive role for the microtubule in Dam1 ring formation. Integrating this information with previously published data, we generated a structural model for the Dam1 complex assembly that advances our understanding of its function and will direct future experiments.
format Online
Article
Text
id pubmed-3216659
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-32166592012-01-30 Subunit organization in the Dam1 kinetochore complex and its ring around microtubules Ramey, Vincent H. Wong, Amanda Fang, Jie Howes, Stuart Barnes, Georjana Nogales, Eva Mol Biol Cell Articles All eukaryotic cells must segregate their chromosomes equally between two daughter cells at each division. This process needs to be robust, as errors in the form of loss or gain of genetic material have catastrophic effects on viability. Chromosomes are captured, aligned, and segregated to daughter cells via interaction with spindle microtubules mediated by the kinetochore. In Saccharomyces cerevisiae one microtubule attaches to each kinetochore, requiring extreme processivity from this single connection. The yeast Dam1 complex, an essential component of the outer kinetochore, forms rings around microtubules and in vitro recapitulates much of the functionality of a kinetochore–microtubule attachment. To understand the mechanism of the Dam1 complex at the kinetochore, we must know how it binds to microtubules, how it assembles into rings, and how assembly is regulated. We used electron microscopy to map several subunits within the structure of the Dam1 complex and identify the organization of Dam1 complexes within the ring. Of importance, new data strongly support a more passive role for the microtubule in Dam1 ring formation. Integrating this information with previously published data, we generated a structural model for the Dam1 complex assembly that advances our understanding of its function and will direct future experiments. The American Society for Cell Biology 2011-11-15 /pmc/articles/PMC3216659/ /pubmed/21965284 http://dx.doi.org/10.1091/mbc.E11-07-0659 Text en © 2011 Ramey et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Ramey, Vincent H.
Wong, Amanda
Fang, Jie
Howes, Stuart
Barnes, Georjana
Nogales, Eva
Subunit organization in the Dam1 kinetochore complex and its ring around microtubules
title Subunit organization in the Dam1 kinetochore complex and its ring around microtubules
title_full Subunit organization in the Dam1 kinetochore complex and its ring around microtubules
title_fullStr Subunit organization in the Dam1 kinetochore complex and its ring around microtubules
title_full_unstemmed Subunit organization in the Dam1 kinetochore complex and its ring around microtubules
title_short Subunit organization in the Dam1 kinetochore complex and its ring around microtubules
title_sort subunit organization in the dam1 kinetochore complex and its ring around microtubules
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216659/
https://www.ncbi.nlm.nih.gov/pubmed/21965284
http://dx.doi.org/10.1091/mbc.E11-07-0659
work_keys_str_mv AT rameyvincenth subunitorganizationinthedam1kinetochorecomplexanditsringaroundmicrotubules
AT wongamanda subunitorganizationinthedam1kinetochorecomplexanditsringaroundmicrotubules
AT fangjie subunitorganizationinthedam1kinetochorecomplexanditsringaroundmicrotubules
AT howesstuart subunitorganizationinthedam1kinetochorecomplexanditsringaroundmicrotubules
AT barnesgeorjana subunitorganizationinthedam1kinetochorecomplexanditsringaroundmicrotubules
AT nogaleseva subunitorganizationinthedam1kinetochorecomplexanditsringaroundmicrotubules