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Subunit organization in the Dam1 kinetochore complex and its ring around microtubules
All eukaryotic cells must segregate their chromosomes equally between two daughter cells at each division. This process needs to be robust, as errors in the form of loss or gain of genetic material have catastrophic effects on viability. Chromosomes are captured, aligned, and segregated to daughter...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216659/ https://www.ncbi.nlm.nih.gov/pubmed/21965284 http://dx.doi.org/10.1091/mbc.E11-07-0659 |
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author | Ramey, Vincent H. Wong, Amanda Fang, Jie Howes, Stuart Barnes, Georjana Nogales, Eva |
author_facet | Ramey, Vincent H. Wong, Amanda Fang, Jie Howes, Stuart Barnes, Georjana Nogales, Eva |
author_sort | Ramey, Vincent H. |
collection | PubMed |
description | All eukaryotic cells must segregate their chromosomes equally between two daughter cells at each division. This process needs to be robust, as errors in the form of loss or gain of genetic material have catastrophic effects on viability. Chromosomes are captured, aligned, and segregated to daughter cells via interaction with spindle microtubules mediated by the kinetochore. In Saccharomyces cerevisiae one microtubule attaches to each kinetochore, requiring extreme processivity from this single connection. The yeast Dam1 complex, an essential component of the outer kinetochore, forms rings around microtubules and in vitro recapitulates much of the functionality of a kinetochore–microtubule attachment. To understand the mechanism of the Dam1 complex at the kinetochore, we must know how it binds to microtubules, how it assembles into rings, and how assembly is regulated. We used electron microscopy to map several subunits within the structure of the Dam1 complex and identify the organization of Dam1 complexes within the ring. Of importance, new data strongly support a more passive role for the microtubule in Dam1 ring formation. Integrating this information with previously published data, we generated a structural model for the Dam1 complex assembly that advances our understanding of its function and will direct future experiments. |
format | Online Article Text |
id | pubmed-3216659 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-32166592012-01-30 Subunit organization in the Dam1 kinetochore complex and its ring around microtubules Ramey, Vincent H. Wong, Amanda Fang, Jie Howes, Stuart Barnes, Georjana Nogales, Eva Mol Biol Cell Articles All eukaryotic cells must segregate their chromosomes equally between two daughter cells at each division. This process needs to be robust, as errors in the form of loss or gain of genetic material have catastrophic effects on viability. Chromosomes are captured, aligned, and segregated to daughter cells via interaction with spindle microtubules mediated by the kinetochore. In Saccharomyces cerevisiae one microtubule attaches to each kinetochore, requiring extreme processivity from this single connection. The yeast Dam1 complex, an essential component of the outer kinetochore, forms rings around microtubules and in vitro recapitulates much of the functionality of a kinetochore–microtubule attachment. To understand the mechanism of the Dam1 complex at the kinetochore, we must know how it binds to microtubules, how it assembles into rings, and how assembly is regulated. We used electron microscopy to map several subunits within the structure of the Dam1 complex and identify the organization of Dam1 complexes within the ring. Of importance, new data strongly support a more passive role for the microtubule in Dam1 ring formation. Integrating this information with previously published data, we generated a structural model for the Dam1 complex assembly that advances our understanding of its function and will direct future experiments. The American Society for Cell Biology 2011-11-15 /pmc/articles/PMC3216659/ /pubmed/21965284 http://dx.doi.org/10.1091/mbc.E11-07-0659 Text en © 2011 Ramey et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Ramey, Vincent H. Wong, Amanda Fang, Jie Howes, Stuart Barnes, Georjana Nogales, Eva Subunit organization in the Dam1 kinetochore complex and its ring around microtubules |
title | Subunit organization in the Dam1 kinetochore complex and its ring around microtubules |
title_full | Subunit organization in the Dam1 kinetochore complex and its ring around microtubules |
title_fullStr | Subunit organization in the Dam1 kinetochore complex and its ring around microtubules |
title_full_unstemmed | Subunit organization in the Dam1 kinetochore complex and its ring around microtubules |
title_short | Subunit organization in the Dam1 kinetochore complex and its ring around microtubules |
title_sort | subunit organization in the dam1 kinetochore complex and its ring around microtubules |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216659/ https://www.ncbi.nlm.nih.gov/pubmed/21965284 http://dx.doi.org/10.1091/mbc.E11-07-0659 |
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