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Apical–basal polarity in Drosophila neuroblasts is independent of vesicular trafficking

The possession of apical–basal polarity is a common feature of epithelia and neural stem cells, so-called neuroblasts (NBs). In Drosophila, an evolutionarily conserved protein complex consisting of atypical protein kinase C and the scaffolding proteins Bazooka/PAR-3 and PAR-6 controls the polarity o...

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Autores principales: Halbsgut, Nils, Linnemannstöns, Karen, Zimmermann, Laura Isabel, Wodarz, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216662/
https://www.ncbi.nlm.nih.gov/pubmed/21937725
http://dx.doi.org/10.1091/mbc.E11-03-0219
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author Halbsgut, Nils
Linnemannstöns, Karen
Zimmermann, Laura Isabel
Wodarz, Andreas
author_facet Halbsgut, Nils
Linnemannstöns, Karen
Zimmermann, Laura Isabel
Wodarz, Andreas
author_sort Halbsgut, Nils
collection PubMed
description The possession of apical–basal polarity is a common feature of epithelia and neural stem cells, so-called neuroblasts (NBs). In Drosophila, an evolutionarily conserved protein complex consisting of atypical protein kinase C and the scaffolding proteins Bazooka/PAR-3 and PAR-6 controls the polarity of both cell types. The components of this complex localize to the apical junctional region of epithelial cells and form an apical crescent in NBs. In epithelia, the PAR proteins interact with the cellular machinery for polarized exocytosis and endocytosis, both of which are essential for the establishment of plasma membrane polarity. In NBs, many cortical proteins show a strongly polarized subcellular localization, but there is little evidence for the existence of distinct apical and basolateral plasma membrane domains, raising the question of whether vesicular trafficking is required for polarization of NBs. We analyzed the polarity of NBs mutant for essential regulators of the main exocytic and endocytic pathways. Surprisingly, we found that none of these mutations affected NB polarity, demonstrating that NB cortical polarity is independent of plasma membrane polarity and that the PAR proteins function in a cell type–specific manner.
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spelling pubmed-32166622012-01-30 Apical–basal polarity in Drosophila neuroblasts is independent of vesicular trafficking Halbsgut, Nils Linnemannstöns, Karen Zimmermann, Laura Isabel Wodarz, Andreas Mol Biol Cell Articles The possession of apical–basal polarity is a common feature of epithelia and neural stem cells, so-called neuroblasts (NBs). In Drosophila, an evolutionarily conserved protein complex consisting of atypical protein kinase C and the scaffolding proteins Bazooka/PAR-3 and PAR-6 controls the polarity of both cell types. The components of this complex localize to the apical junctional region of epithelial cells and form an apical crescent in NBs. In epithelia, the PAR proteins interact with the cellular machinery for polarized exocytosis and endocytosis, both of which are essential for the establishment of plasma membrane polarity. In NBs, many cortical proteins show a strongly polarized subcellular localization, but there is little evidence for the existence of distinct apical and basolateral plasma membrane domains, raising the question of whether vesicular trafficking is required for polarization of NBs. We analyzed the polarity of NBs mutant for essential regulators of the main exocytic and endocytic pathways. Surprisingly, we found that none of these mutations affected NB polarity, demonstrating that NB cortical polarity is independent of plasma membrane polarity and that the PAR proteins function in a cell type–specific manner. The American Society for Cell Biology 2011-11-15 /pmc/articles/PMC3216662/ /pubmed/21937725 http://dx.doi.org/10.1091/mbc.E11-03-0219 Text en © 2011 Halbsgut et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Halbsgut, Nils
Linnemannstöns, Karen
Zimmermann, Laura Isabel
Wodarz, Andreas
Apical–basal polarity in Drosophila neuroblasts is independent of vesicular trafficking
title Apical–basal polarity in Drosophila neuroblasts is independent of vesicular trafficking
title_full Apical–basal polarity in Drosophila neuroblasts is independent of vesicular trafficking
title_fullStr Apical–basal polarity in Drosophila neuroblasts is independent of vesicular trafficking
title_full_unstemmed Apical–basal polarity in Drosophila neuroblasts is independent of vesicular trafficking
title_short Apical–basal polarity in Drosophila neuroblasts is independent of vesicular trafficking
title_sort apical–basal polarity in drosophila neuroblasts is independent of vesicular trafficking
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216662/
https://www.ncbi.nlm.nih.gov/pubmed/21937725
http://dx.doi.org/10.1091/mbc.E11-03-0219
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