Fast-acting and nearly gratuitous induction of gene expression and protein depletion in Saccharomyces cerevisiae

We describe the development and characterization of a system that allows the rapid and specific induction of individual genes in the yeast Saccharomyces cerevisiae without changes in nutrients or temperature. The system is based on the chimeric transcriptional activator Gal4dbd.ER.VP16 (GEV). Upon a...

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Detalles Bibliográficos
Autores principales: McIsaac, R. Scott, Silverman, Sanford J., McClean, Megan N., Gibney, Patrick A., Macinskas, Joanna, Hickman, Mark J., Petti, Allegra A., Botstein, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216669/
https://www.ncbi.nlm.nih.gov/pubmed/21965290
http://dx.doi.org/10.1091/mbc.E11-05-0466

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216669/
https://www.ncbi.nlm.nih.gov/pubmed/21965290
http://dx.doi.org/10.1091/mbc.E11-05-0466

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