Cargando…

Inhibition of carcinogen induced c-Ha-ras and c-fos proto-oncogenes expression by dietary curcumin

BACKGROUND: We investigated the chemopreventive action of dietary curcumin on 7,12-dimethylbenz(a)anthracene (DMBA)-initiated and 12,0-tetradecanoylphorbol-13-acetate (TPA)-promoted skin tumor formation in Swiss albino mice. Curcumin, a yellow coloring matter isolated from roots of Curcuma longa Lin...

Descripción completa

Detalles Bibliográficos
Autores principales: Limtrakul, Porn-ngarm, Anuchapreeda, Songyot, Lipigorngoson, Suwiwek, Dunn, Floyd W
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC32169/
https://www.ncbi.nlm.nih.gov/pubmed/11231886
http://dx.doi.org/10.1186/1471-2407-1-1
_version_ 1782119986177769472
author Limtrakul, Porn-ngarm
Anuchapreeda, Songyot
Lipigorngoson, Suwiwek
Dunn, Floyd W
author_facet Limtrakul, Porn-ngarm
Anuchapreeda, Songyot
Lipigorngoson, Suwiwek
Dunn, Floyd W
author_sort Limtrakul, Porn-ngarm
collection PubMed
description BACKGROUND: We investigated the chemopreventive action of dietary curcumin on 7,12-dimethylbenz(a)anthracene (DMBA)-initiated and 12,0-tetradecanoylphorbol-13-acetate (TPA)-promoted skin tumor formation in Swiss albino mice. Curcumin, a yellow coloring matter isolated from roots of Curcuma longa Linn, is a phenolic compound possessing antioxidant, free radical scavenger, and antiinflammatory properties. It has been shown by previously reported work that TPA-induced skin tumors were inhibited by topical application of curcumin, and curcumin has been shown to inhibit a variety of biological activities of TPA. Topical application of curcumin was reported to inhibit TPA-induced c-fos, c-jun and c-myc gene expression in mouse skin. This paper reports the effects of orally administered curcumin, which was consumed as a dietary component at concentrations of 0.2 % or 1 %, in ad libitum feeding. RESULTS: Animals in which tumors had been initiated with DMBA and promoted with TPA experienced significantly fewer tumors and less tumor volume if they ingested either 0.2% or 1% curcumin diets. Also, the dietary consumption of curcumin resulted in a significantly decreased expression of ras and fos proto-oncogenes in the tumorous skin, as measured by enhanced chemiluminesence Western blotting detection system (Amersham). CONCLUSIONS: Whereas earlier work demonstrated that topical application of curcumin to mouse skin inhibited TPA-induced expression of c-fos, c-jun and c-myc oncogenes, our results are the first to show that orally consumed curcumin significantly inhibited DMBA- and TPA-induced ras and fos gene expression in mouse skin.
format Text
id pubmed-32169
institution National Center for Biotechnology Information
language English
publishDate 2001
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-321692001-06-01 Inhibition of carcinogen induced c-Ha-ras and c-fos proto-oncogenes expression by dietary curcumin Limtrakul, Porn-ngarm Anuchapreeda, Songyot Lipigorngoson, Suwiwek Dunn, Floyd W BMC Cancer Research Article BACKGROUND: We investigated the chemopreventive action of dietary curcumin on 7,12-dimethylbenz(a)anthracene (DMBA)-initiated and 12,0-tetradecanoylphorbol-13-acetate (TPA)-promoted skin tumor formation in Swiss albino mice. Curcumin, a yellow coloring matter isolated from roots of Curcuma longa Linn, is a phenolic compound possessing antioxidant, free radical scavenger, and antiinflammatory properties. It has been shown by previously reported work that TPA-induced skin tumors were inhibited by topical application of curcumin, and curcumin has been shown to inhibit a variety of biological activities of TPA. Topical application of curcumin was reported to inhibit TPA-induced c-fos, c-jun and c-myc gene expression in mouse skin. This paper reports the effects of orally administered curcumin, which was consumed as a dietary component at concentrations of 0.2 % or 1 %, in ad libitum feeding. RESULTS: Animals in which tumors had been initiated with DMBA and promoted with TPA experienced significantly fewer tumors and less tumor volume if they ingested either 0.2% or 1% curcumin diets. Also, the dietary consumption of curcumin resulted in a significantly decreased expression of ras and fos proto-oncogenes in the tumorous skin, as measured by enhanced chemiluminesence Western blotting detection system (Amersham). CONCLUSIONS: Whereas earlier work demonstrated that topical application of curcumin to mouse skin inhibited TPA-induced expression of c-fos, c-jun and c-myc oncogenes, our results are the first to show that orally consumed curcumin significantly inhibited DMBA- and TPA-induced ras and fos gene expression in mouse skin. BioMed Central 2001-01-17 /pmc/articles/PMC32169/ /pubmed/11231886 http://dx.doi.org/10.1186/1471-2407-1-1 Text en Copyright © 2001 Limtrakul et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Research Article
Limtrakul, Porn-ngarm
Anuchapreeda, Songyot
Lipigorngoson, Suwiwek
Dunn, Floyd W
Inhibition of carcinogen induced c-Ha-ras and c-fos proto-oncogenes expression by dietary curcumin
title Inhibition of carcinogen induced c-Ha-ras and c-fos proto-oncogenes expression by dietary curcumin
title_full Inhibition of carcinogen induced c-Ha-ras and c-fos proto-oncogenes expression by dietary curcumin
title_fullStr Inhibition of carcinogen induced c-Ha-ras and c-fos proto-oncogenes expression by dietary curcumin
title_full_unstemmed Inhibition of carcinogen induced c-Ha-ras and c-fos proto-oncogenes expression by dietary curcumin
title_short Inhibition of carcinogen induced c-Ha-ras and c-fos proto-oncogenes expression by dietary curcumin
title_sort inhibition of carcinogen induced c-ha-ras and c-fos proto-oncogenes expression by dietary curcumin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC32169/
https://www.ncbi.nlm.nih.gov/pubmed/11231886
http://dx.doi.org/10.1186/1471-2407-1-1
work_keys_str_mv AT limtrakulpornngarm inhibitionofcarcinogeninducedcharasandcfosprotooncogenesexpressionbydietarycurcumin
AT anuchapreedasongyot inhibitionofcarcinogeninducedcharasandcfosprotooncogenesexpressionbydietarycurcumin
AT lipigorngosonsuwiwek inhibitionofcarcinogeninducedcharasandcfosprotooncogenesexpressionbydietarycurcumin
AT dunnfloydw inhibitionofcarcinogeninducedcharasandcfosprotooncogenesexpressionbydietarycurcumin