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MicroRNA Expression in Abdominal and Gluteal Adipose Tissue Is Associated with mRNA Expression Levels and Partly Genetically Driven

To understand how miRNAs contribute to the molecular phenotype of adipose tissues and related traits, we performed global miRNA expression profiling in subcutaneous abdominal and gluteal adipose tissue of 70 human subjects and characterised which miRNAs were differentially expressed between these ti...

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Autores principales: Rantalainen, Mattias, Herrera, Blanca M., Nicholson, George, Bowden, Rory, Wills, Quin F., Min, Josine L., Neville, Matt J., Barrett, Amy, Allen, Maxine, Rayner, Nigel W., Fleckner, Jan, McCarthy, Mark I., Zondervan, Krina T., Karpe, Fredrik, Holmes, Chris C., Lindgren, Cecilia M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216936/
https://www.ncbi.nlm.nih.gov/pubmed/22102887
http://dx.doi.org/10.1371/journal.pone.0027338
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author Rantalainen, Mattias
Herrera, Blanca M.
Nicholson, George
Bowden, Rory
Wills, Quin F.
Min, Josine L.
Neville, Matt J.
Barrett, Amy
Allen, Maxine
Rayner, Nigel W.
Fleckner, Jan
McCarthy, Mark I.
Zondervan, Krina T.
Karpe, Fredrik
Holmes, Chris C.
Lindgren, Cecilia M.
author_facet Rantalainen, Mattias
Herrera, Blanca M.
Nicholson, George
Bowden, Rory
Wills, Quin F.
Min, Josine L.
Neville, Matt J.
Barrett, Amy
Allen, Maxine
Rayner, Nigel W.
Fleckner, Jan
McCarthy, Mark I.
Zondervan, Krina T.
Karpe, Fredrik
Holmes, Chris C.
Lindgren, Cecilia M.
author_sort Rantalainen, Mattias
collection PubMed
description To understand how miRNAs contribute to the molecular phenotype of adipose tissues and related traits, we performed global miRNA expression profiling in subcutaneous abdominal and gluteal adipose tissue of 70 human subjects and characterised which miRNAs were differentially expressed between these tissues. We found that 12% of the miRNAs were significantly differentially expressed between abdominal and gluteal adipose tissue (FDR adjusted p<0.05) in the primary study, of which 59 replicated in a follow-up study of 40 additional subjects. Further, 14 miRNAs were found to be associated with metabolic syndrome case-control status in abdominal tissue and three of these replicated (primary study: FDR adjusted p<0.05, replication: p<0.05 and directionally consistent effect). Genome-wide genotyping was performed in the 70 subjects to enable miRNA expression quantitative trait loci (eQTL) analysis. Candidate miRNA eQTLs were followed-up in the additional 40 subjects and six significant, independent cis-located miRNA eQTLs (primary study: p<0.001; replication: p<0.05 and directionally consistent effect) were identified. Finally, global mRNA expression profiling was performed in both tissues to enable association analysis between miRNA and target mRNA expression levels. We find 22% miRNAs in abdominal and 9% miRNAs in gluteal adipose tissue with expression levels significantly associated with the expression of corresponding target mRNAs (FDR adjusted p<0.05). Taken together, our results indicate a clear difference in the miRNA molecular phenotypic profile of abdominal and gluteal adipose tissue, that the expressions of some miRNAs are influenced by cis-located genetic variants and that miRNAs are associated with expression levels of their predicted mRNA targets.
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spelling pubmed-32169362011-11-18 MicroRNA Expression in Abdominal and Gluteal Adipose Tissue Is Associated with mRNA Expression Levels and Partly Genetically Driven Rantalainen, Mattias Herrera, Blanca M. Nicholson, George Bowden, Rory Wills, Quin F. Min, Josine L. Neville, Matt J. Barrett, Amy Allen, Maxine Rayner, Nigel W. Fleckner, Jan McCarthy, Mark I. Zondervan, Krina T. Karpe, Fredrik Holmes, Chris C. Lindgren, Cecilia M. PLoS One Research Article To understand how miRNAs contribute to the molecular phenotype of adipose tissues and related traits, we performed global miRNA expression profiling in subcutaneous abdominal and gluteal adipose tissue of 70 human subjects and characterised which miRNAs were differentially expressed between these tissues. We found that 12% of the miRNAs were significantly differentially expressed between abdominal and gluteal adipose tissue (FDR adjusted p<0.05) in the primary study, of which 59 replicated in a follow-up study of 40 additional subjects. Further, 14 miRNAs were found to be associated with metabolic syndrome case-control status in abdominal tissue and three of these replicated (primary study: FDR adjusted p<0.05, replication: p<0.05 and directionally consistent effect). Genome-wide genotyping was performed in the 70 subjects to enable miRNA expression quantitative trait loci (eQTL) analysis. Candidate miRNA eQTLs were followed-up in the additional 40 subjects and six significant, independent cis-located miRNA eQTLs (primary study: p<0.001; replication: p<0.05 and directionally consistent effect) were identified. Finally, global mRNA expression profiling was performed in both tissues to enable association analysis between miRNA and target mRNA expression levels. We find 22% miRNAs in abdominal and 9% miRNAs in gluteal adipose tissue with expression levels significantly associated with the expression of corresponding target mRNAs (FDR adjusted p<0.05). Taken together, our results indicate a clear difference in the miRNA molecular phenotypic profile of abdominal and gluteal adipose tissue, that the expressions of some miRNAs are influenced by cis-located genetic variants and that miRNAs are associated with expression levels of their predicted mRNA targets. Public Library of Science 2011-11-15 /pmc/articles/PMC3216936/ /pubmed/22102887 http://dx.doi.org/10.1371/journal.pone.0027338 Text en Rantalainen et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Rantalainen, Mattias
Herrera, Blanca M.
Nicholson, George
Bowden, Rory
Wills, Quin F.
Min, Josine L.
Neville, Matt J.
Barrett, Amy
Allen, Maxine
Rayner, Nigel W.
Fleckner, Jan
McCarthy, Mark I.
Zondervan, Krina T.
Karpe, Fredrik
Holmes, Chris C.
Lindgren, Cecilia M.
MicroRNA Expression in Abdominal and Gluteal Adipose Tissue Is Associated with mRNA Expression Levels and Partly Genetically Driven
title MicroRNA Expression in Abdominal and Gluteal Adipose Tissue Is Associated with mRNA Expression Levels and Partly Genetically Driven
title_full MicroRNA Expression in Abdominal and Gluteal Adipose Tissue Is Associated with mRNA Expression Levels and Partly Genetically Driven
title_fullStr MicroRNA Expression in Abdominal and Gluteal Adipose Tissue Is Associated with mRNA Expression Levels and Partly Genetically Driven
title_full_unstemmed MicroRNA Expression in Abdominal and Gluteal Adipose Tissue Is Associated with mRNA Expression Levels and Partly Genetically Driven
title_short MicroRNA Expression in Abdominal and Gluteal Adipose Tissue Is Associated with mRNA Expression Levels and Partly Genetically Driven
title_sort microrna expression in abdominal and gluteal adipose tissue is associated with mrna expression levels and partly genetically driven
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216936/
https://www.ncbi.nlm.nih.gov/pubmed/22102887
http://dx.doi.org/10.1371/journal.pone.0027338
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