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Characterization of Five Fungal Endophytes Producing Cajaninstilbene Acid Isolated from Pigeon Pea [Cajanus cajan (L.) Millsp.]

Five fungal endophytes (K4, K5, K6, K9, K14) producing Cajaninstilbene acid (CSA, 3-hydroxy-4-prenyl-5-methoxystilbene-2-carboxylic acid) were isolated from the roots of pigeon pea [Cajanus cajan (L.) Millsp.]. CSA is responsible for the prominent pharmacological activities in pigeon pea. The amount...

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Autores principales: Gao, Yuan, Zhao, Jin Tong, Zu, Yuan Gang, Fu, Yu Jie, Wang, Wei, Luo, Meng, Efferth, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216958/
https://www.ncbi.nlm.nih.gov/pubmed/22102911
http://dx.doi.org/10.1371/journal.pone.0027589
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author Gao, Yuan
Zhao, Jin Tong
Zu, Yuan Gang
Fu, Yu Jie
Wang, Wei
Luo, Meng
Efferth, Thomas
author_facet Gao, Yuan
Zhao, Jin Tong
Zu, Yuan Gang
Fu, Yu Jie
Wang, Wei
Luo, Meng
Efferth, Thomas
author_sort Gao, Yuan
collection PubMed
description Five fungal endophytes (K4, K5, K6, K9, K14) producing Cajaninstilbene acid (CSA, 3-hydroxy-4-prenyl-5-methoxystilbene-2-carboxylic acid) were isolated from the roots of pigeon pea [Cajanus cajan (L.) Millsp.]. CSA is responsible for the prominent pharmacological activities in pigeon pea. The amount of CSA in culture solution varied among the five fungal endophytes. K4 produced the highest levels of CSA (1037.13 µg/L) among the endophytes tested after incubation for five days. Both morphological characteristics and molecular methods were used for species identification of fungal endophytes. The five endophytic isolates were characterized by analyzing the internal transcribed spacer (ITS) rRNA and β-tubulin genes. The K4, K5, K9 and K14 strains isolated from pigeon pea roots were found to be closely related to the species Fusarium oxysporum. K6 was identified as Neonectria macrodidym. The present study is the first report on the isolation and identification of fungal endophytes producing CSA in pigeon pea. The study also provides a scientific base for large scale production of CSA.
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spelling pubmed-32169582011-11-18 Characterization of Five Fungal Endophytes Producing Cajaninstilbene Acid Isolated from Pigeon Pea [Cajanus cajan (L.) Millsp.] Gao, Yuan Zhao, Jin Tong Zu, Yuan Gang Fu, Yu Jie Wang, Wei Luo, Meng Efferth, Thomas PLoS One Research Article Five fungal endophytes (K4, K5, K6, K9, K14) producing Cajaninstilbene acid (CSA, 3-hydroxy-4-prenyl-5-methoxystilbene-2-carboxylic acid) were isolated from the roots of pigeon pea [Cajanus cajan (L.) Millsp.]. CSA is responsible for the prominent pharmacological activities in pigeon pea. The amount of CSA in culture solution varied among the five fungal endophytes. K4 produced the highest levels of CSA (1037.13 µg/L) among the endophytes tested after incubation for five days. Both morphological characteristics and molecular methods were used for species identification of fungal endophytes. The five endophytic isolates were characterized by analyzing the internal transcribed spacer (ITS) rRNA and β-tubulin genes. The K4, K5, K9 and K14 strains isolated from pigeon pea roots were found to be closely related to the species Fusarium oxysporum. K6 was identified as Neonectria macrodidym. The present study is the first report on the isolation and identification of fungal endophytes producing CSA in pigeon pea. The study also provides a scientific base for large scale production of CSA. Public Library of Science 2011-11-15 /pmc/articles/PMC3216958/ /pubmed/22102911 http://dx.doi.org/10.1371/journal.pone.0027589 Text en Gao et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Gao, Yuan
Zhao, Jin Tong
Zu, Yuan Gang
Fu, Yu Jie
Wang, Wei
Luo, Meng
Efferth, Thomas
Characterization of Five Fungal Endophytes Producing Cajaninstilbene Acid Isolated from Pigeon Pea [Cajanus cajan (L.) Millsp.]
title Characterization of Five Fungal Endophytes Producing Cajaninstilbene Acid Isolated from Pigeon Pea [Cajanus cajan (L.) Millsp.]
title_full Characterization of Five Fungal Endophytes Producing Cajaninstilbene Acid Isolated from Pigeon Pea [Cajanus cajan (L.) Millsp.]
title_fullStr Characterization of Five Fungal Endophytes Producing Cajaninstilbene Acid Isolated from Pigeon Pea [Cajanus cajan (L.) Millsp.]
title_full_unstemmed Characterization of Five Fungal Endophytes Producing Cajaninstilbene Acid Isolated from Pigeon Pea [Cajanus cajan (L.) Millsp.]
title_short Characterization of Five Fungal Endophytes Producing Cajaninstilbene Acid Isolated from Pigeon Pea [Cajanus cajan (L.) Millsp.]
title_sort characterization of five fungal endophytes producing cajaninstilbene acid isolated from pigeon pea [cajanus cajan (l.) millsp.]
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3216958/
https://www.ncbi.nlm.nih.gov/pubmed/22102911
http://dx.doi.org/10.1371/journal.pone.0027589
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