Post-Translational Modifications and Lipid Binding Profile of Insect Cell-Expressed Full-Length Mammalian Synaptotagmin 1

[Image: see text] Synaptotagmin 1 (Syt1) is a Ca(2+) sensor for SNARE-mediated, Ca(2+)-triggered synaptic vesicle fusion in neurons. It is composed of luminal, transmembrane, linker, and two Ca(2+)-binding (C2) domains. Here we describe expression and purification of full-length mammalian Syt1 in in...

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Detalles Bibliográficos
Autores principales: Vrljic, Marija, Strop, Pavel, Hill, Ryan C., Hansen, Kirk C., Chu, Steven, Brunger, Axel T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2011
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3217305/
https://www.ncbi.nlm.nih.gov/pubmed/21928778
http://dx.doi.org/10.1021/bi200998y

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