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Antioxidant properties of lutein contribute to the protection against lipopolysaccharide-induced uveitis in mice

BACKGROUND: Lutein is an important eye-protective nutrient. This study investigates the protective effects and mechanisms of lutein on lipopolysaccharides (LPS)-induced uveitis in mice. METHODS: Lutein, suspended in drinking water at a final concentration of 12.5 and 25 mg/mL, was administered to mi...

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Autores principales: He, Rong-Rong, Tsoi, Bun, Lan, Fang, Yao, Nan, Yao, Xin-Sheng, Kurihara, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3217882/
https://www.ncbi.nlm.nih.gov/pubmed/22040935
http://dx.doi.org/10.1186/1749-8546-6-38
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author He, Rong-Rong
Tsoi, Bun
Lan, Fang
Yao, Nan
Yao, Xin-Sheng
Kurihara, Hiroshi
author_facet He, Rong-Rong
Tsoi, Bun
Lan, Fang
Yao, Nan
Yao, Xin-Sheng
Kurihara, Hiroshi
author_sort He, Rong-Rong
collection PubMed
description BACKGROUND: Lutein is an important eye-protective nutrient. This study investigates the protective effects and mechanisms of lutein on lipopolysaccharides (LPS)-induced uveitis in mice. METHODS: Lutein, suspended in drinking water at a final concentration of 12.5 and 25 mg/mL, was administered to mice at 0.1 mL/10 g body weight for five consecutive days. Control and model group received drinking water only. Uveitis was induced by injecting LPS (100 mg per mouse) into the footpad in the model and lutein groups on day 5 after the last drug administration. Eyes of the mice were collected 24 hours after the LPS injection for the detection of indicators using commercial kits and reverse transcription-polymerase chain reaction. RESULTS: LPS-induced uveitis was confirmed by significant pathological damage and increased the nitric oxide level in eye tissue of BALB/C mice 24 hours after the footpad injection. The elevated nitric oxide level was significantly reduced by oral administration of lutein (125 and 500 mg/kg/d for five days) before LPS injection. Moreover, lutein decreased the malondialdehyde content, increased the oxygen radical absorbance capacity level, glutathione, the vitamin C contents and total superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities. Lutein further increased expressions of copper-zinc SOD, manganese SOD and GPx mRNA. Conclusion The antioxidant properties of lutein contribute to the protection against LPS-induced uveitis, partially through the intervention of inflammation process.
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spelling pubmed-32178822011-11-17 Antioxidant properties of lutein contribute to the protection against lipopolysaccharide-induced uveitis in mice He, Rong-Rong Tsoi, Bun Lan, Fang Yao, Nan Yao, Xin-Sheng Kurihara, Hiroshi Chin Med Research BACKGROUND: Lutein is an important eye-protective nutrient. This study investigates the protective effects and mechanisms of lutein on lipopolysaccharides (LPS)-induced uveitis in mice. METHODS: Lutein, suspended in drinking water at a final concentration of 12.5 and 25 mg/mL, was administered to mice at 0.1 mL/10 g body weight for five consecutive days. Control and model group received drinking water only. Uveitis was induced by injecting LPS (100 mg per mouse) into the footpad in the model and lutein groups on day 5 after the last drug administration. Eyes of the mice were collected 24 hours after the LPS injection for the detection of indicators using commercial kits and reverse transcription-polymerase chain reaction. RESULTS: LPS-induced uveitis was confirmed by significant pathological damage and increased the nitric oxide level in eye tissue of BALB/C mice 24 hours after the footpad injection. The elevated nitric oxide level was significantly reduced by oral administration of lutein (125 and 500 mg/kg/d for five days) before LPS injection. Moreover, lutein decreased the malondialdehyde content, increased the oxygen radical absorbance capacity level, glutathione, the vitamin C contents and total superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities. Lutein further increased expressions of copper-zinc SOD, manganese SOD and GPx mRNA. Conclusion The antioxidant properties of lutein contribute to the protection against LPS-induced uveitis, partially through the intervention of inflammation process. BioMed Central 2011-10-31 /pmc/articles/PMC3217882/ /pubmed/22040935 http://dx.doi.org/10.1186/1749-8546-6-38 Text en Copyright ©2011 He et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
He, Rong-Rong
Tsoi, Bun
Lan, Fang
Yao, Nan
Yao, Xin-Sheng
Kurihara, Hiroshi
Antioxidant properties of lutein contribute to the protection against lipopolysaccharide-induced uveitis in mice
title Antioxidant properties of lutein contribute to the protection against lipopolysaccharide-induced uveitis in mice
title_full Antioxidant properties of lutein contribute to the protection against lipopolysaccharide-induced uveitis in mice
title_fullStr Antioxidant properties of lutein contribute to the protection against lipopolysaccharide-induced uveitis in mice
title_full_unstemmed Antioxidant properties of lutein contribute to the protection against lipopolysaccharide-induced uveitis in mice
title_short Antioxidant properties of lutein contribute to the protection against lipopolysaccharide-induced uveitis in mice
title_sort antioxidant properties of lutein contribute to the protection against lipopolysaccharide-induced uveitis in mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3217882/
https://www.ncbi.nlm.nih.gov/pubmed/22040935
http://dx.doi.org/10.1186/1749-8546-6-38
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