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A Conserved GA Element in TATA-Less RNA Polymerase II Promoters

Initiation of RNA polymerase (Pol) II transcription requires assembly of the pre-initiation complex (PIC) at the promoter. In the classical view, PIC assembly starts with binding of the TATA box-binding protein (TBP) to the TATA box. However, a TATA box occurs in only 15% of promoters in the yeast S...

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Autores principales: Seizl, Martin, Hartmann, Holger, Hoeg, Friederike, Kurth, Fabian, Martin, Dietmar E., Söding, Johannes, Cramer, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3217976/
https://www.ncbi.nlm.nih.gov/pubmed/22110682
http://dx.doi.org/10.1371/journal.pone.0027595
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author Seizl, Martin
Hartmann, Holger
Hoeg, Friederike
Kurth, Fabian
Martin, Dietmar E.
Söding, Johannes
Cramer, Patrick
author_facet Seizl, Martin
Hartmann, Holger
Hoeg, Friederike
Kurth, Fabian
Martin, Dietmar E.
Söding, Johannes
Cramer, Patrick
author_sort Seizl, Martin
collection PubMed
description Initiation of RNA polymerase (Pol) II transcription requires assembly of the pre-initiation complex (PIC) at the promoter. In the classical view, PIC assembly starts with binding of the TATA box-binding protein (TBP) to the TATA box. However, a TATA box occurs in only 15% of promoters in the yeast Saccharomyces cerevisiae, posing the question how most yeast promoters nucleate PIC assembly. Here we show that one third of all yeast promoters contain a novel conserved DNA element, the GA element (GAE), that generally does not co-occur with the TATA box. The distance of the GAE to the transcription start site (TSS) resembles the distance of the TATA box to the TSS. The TATA-less TMT1 core promoter contains a GAE, recruits TBP, and supports formation of a TBP-TFIIB-DNA-complex. Mutation of the promoter region surrounding the GAE abolishes transcription in vivo and in vitro. A 32-nucleotide promoter region containing the GAE can functionally substitute for the TATA box in a TATA-containing promoter. This identifies the GAE as a conserved promoter element in TATA-less promoters.
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spelling pubmed-32179762011-11-21 A Conserved GA Element in TATA-Less RNA Polymerase II Promoters Seizl, Martin Hartmann, Holger Hoeg, Friederike Kurth, Fabian Martin, Dietmar E. Söding, Johannes Cramer, Patrick PLoS One Research Article Initiation of RNA polymerase (Pol) II transcription requires assembly of the pre-initiation complex (PIC) at the promoter. In the classical view, PIC assembly starts with binding of the TATA box-binding protein (TBP) to the TATA box. However, a TATA box occurs in only 15% of promoters in the yeast Saccharomyces cerevisiae, posing the question how most yeast promoters nucleate PIC assembly. Here we show that one third of all yeast promoters contain a novel conserved DNA element, the GA element (GAE), that generally does not co-occur with the TATA box. The distance of the GAE to the transcription start site (TSS) resembles the distance of the TATA box to the TSS. The TATA-less TMT1 core promoter contains a GAE, recruits TBP, and supports formation of a TBP-TFIIB-DNA-complex. Mutation of the promoter region surrounding the GAE abolishes transcription in vivo and in vitro. A 32-nucleotide promoter region containing the GAE can functionally substitute for the TATA box in a TATA-containing promoter. This identifies the GAE as a conserved promoter element in TATA-less promoters. Public Library of Science 2011-11-16 /pmc/articles/PMC3217976/ /pubmed/22110682 http://dx.doi.org/10.1371/journal.pone.0027595 Text en Seizl et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Seizl, Martin
Hartmann, Holger
Hoeg, Friederike
Kurth, Fabian
Martin, Dietmar E.
Söding, Johannes
Cramer, Patrick
A Conserved GA Element in TATA-Less RNA Polymerase II Promoters
title A Conserved GA Element in TATA-Less RNA Polymerase II Promoters
title_full A Conserved GA Element in TATA-Less RNA Polymerase II Promoters
title_fullStr A Conserved GA Element in TATA-Less RNA Polymerase II Promoters
title_full_unstemmed A Conserved GA Element in TATA-Less RNA Polymerase II Promoters
title_short A Conserved GA Element in TATA-Less RNA Polymerase II Promoters
title_sort conserved ga element in tata-less rna polymerase ii promoters
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3217976/
https://www.ncbi.nlm.nih.gov/pubmed/22110682
http://dx.doi.org/10.1371/journal.pone.0027595
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