A Retinoic Acid Responsive Hoxa3 Transgene Expressed in Embryonic Pharyngeal Endoderm, Cardiac Neural Crest and a Subdomain of the Second Heart Field

A transgenic mouse line harbouring a β-galacdosidase reporter gene controlled by the proximal 2 kb promoter of Hoxa3 was previously generated to investigate the regulatory cues governing Hoxa3 expression in the mouse. Examination of transgenic embryos from embryonic day (E) 8.0 to E15.5 revealed reg...

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Autores principales: Diman, Nata Y. S.-G., Remacle, Sophie, Bertrand, Nicolas, Picard, Jacques J., Zaffran, Stéphane, Rezsohazy, René
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3217993/
https://www.ncbi.nlm.nih.gov/pubmed/22110697
http://dx.doi.org/10.1371/journal.pone.0027624
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author Diman, Nata Y. S.-G.
Remacle, Sophie
Bertrand, Nicolas
Picard, Jacques J.
Zaffran, Stéphane
Rezsohazy, René
author_facet Diman, Nata Y. S.-G.
Remacle, Sophie
Bertrand, Nicolas
Picard, Jacques J.
Zaffran, Stéphane
Rezsohazy, René
author_sort Diman, Nata Y. S.-G.
collection PubMed
description A transgenic mouse line harbouring a β-galacdosidase reporter gene controlled by the proximal 2 kb promoter of Hoxa3 was previously generated to investigate the regulatory cues governing Hoxa3 expression in the mouse. Examination of transgenic embryos from embryonic day (E) 8.0 to E15.5 revealed regionally restricted reporter activity in the developing heart. Indeed, transgene expression specifically delineated cells from three distinct lineages: a subpopulation of the second heart field contributing to outflow tract myocardium, the cardiac neural crest cells and the pharyngeal endoderm. Manipulation of the Retinoic Acid (RA) signaling pathway showed that RA is required for correct expression of the transgene. Therefore, this transgenic line may serve as a cardiosensor line of particular interest for further analysis of outflow tract development.
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spelling pubmed-32179932011-11-21 A Retinoic Acid Responsive Hoxa3 Transgene Expressed in Embryonic Pharyngeal Endoderm, Cardiac Neural Crest and a Subdomain of the Second Heart Field Diman, Nata Y. S.-G. Remacle, Sophie Bertrand, Nicolas Picard, Jacques J. Zaffran, Stéphane Rezsohazy, René PLoS One Research Article A transgenic mouse line harbouring a β-galacdosidase reporter gene controlled by the proximal 2 kb promoter of Hoxa3 was previously generated to investigate the regulatory cues governing Hoxa3 expression in the mouse. Examination of transgenic embryos from embryonic day (E) 8.0 to E15.5 revealed regionally restricted reporter activity in the developing heart. Indeed, transgene expression specifically delineated cells from three distinct lineages: a subpopulation of the second heart field contributing to outflow tract myocardium, the cardiac neural crest cells and the pharyngeal endoderm. Manipulation of the Retinoic Acid (RA) signaling pathway showed that RA is required for correct expression of the transgene. Therefore, this transgenic line may serve as a cardiosensor line of particular interest for further analysis of outflow tract development. Public Library of Science 2011-11-16 /pmc/articles/PMC3217993/ /pubmed/22110697 http://dx.doi.org/10.1371/journal.pone.0027624 Text en Diman et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Diman, Nata Y. S.-G.
Remacle, Sophie
Bertrand, Nicolas
Picard, Jacques J.
Zaffran, Stéphane
Rezsohazy, René
A Retinoic Acid Responsive Hoxa3 Transgene Expressed in Embryonic Pharyngeal Endoderm, Cardiac Neural Crest and a Subdomain of the Second Heart Field
title A Retinoic Acid Responsive Hoxa3 Transgene Expressed in Embryonic Pharyngeal Endoderm, Cardiac Neural Crest and a Subdomain of the Second Heart Field
title_full A Retinoic Acid Responsive Hoxa3 Transgene Expressed in Embryonic Pharyngeal Endoderm, Cardiac Neural Crest and a Subdomain of the Second Heart Field
title_fullStr A Retinoic Acid Responsive Hoxa3 Transgene Expressed in Embryonic Pharyngeal Endoderm, Cardiac Neural Crest and a Subdomain of the Second Heart Field
title_full_unstemmed A Retinoic Acid Responsive Hoxa3 Transgene Expressed in Embryonic Pharyngeal Endoderm, Cardiac Neural Crest and a Subdomain of the Second Heart Field
title_short A Retinoic Acid Responsive Hoxa3 Transgene Expressed in Embryonic Pharyngeal Endoderm, Cardiac Neural Crest and a Subdomain of the Second Heart Field
title_sort retinoic acid responsive hoxa3 transgene expressed in embryonic pharyngeal endoderm, cardiac neural crest and a subdomain of the second heart field
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3217993/
https://www.ncbi.nlm.nih.gov/pubmed/22110697
http://dx.doi.org/10.1371/journal.pone.0027624
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