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Allergenicity Assessment of Allium sativum Leaf Agglutinin, a Potential Candidate Protein for Developing Sap Sucking Insect Resistant Food Crops

BACKGROUND: Mannose-binding Allium sativum leaf agglutinin (ASAL) is highly antinutritional and toxic to various phloem-feeding hemipteran insects. ASAL has been expressed in a number of agriculturally important crops to develop resistance against those insects. Awareness of the safety aspect of ASA...

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Autores principales: Mondal, Hossain Ali, Chakraborti, Dipankar, Majumder, Pralay, Roy, Pampa, Roy, Amit, Bhattacharya, Swati Gupta, Das, Sampa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3218009/
https://www.ncbi.nlm.nih.gov/pubmed/22110739
http://dx.doi.org/10.1371/journal.pone.0027716
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author Mondal, Hossain Ali
Chakraborti, Dipankar
Majumder, Pralay
Roy, Pampa
Roy, Amit
Bhattacharya, Swati Gupta
Das, Sampa
author_facet Mondal, Hossain Ali
Chakraborti, Dipankar
Majumder, Pralay
Roy, Pampa
Roy, Amit
Bhattacharya, Swati Gupta
Das, Sampa
author_sort Mondal, Hossain Ali
collection PubMed
description BACKGROUND: Mannose-binding Allium sativum leaf agglutinin (ASAL) is highly antinutritional and toxic to various phloem-feeding hemipteran insects. ASAL has been expressed in a number of agriculturally important crops to develop resistance against those insects. Awareness of the safety aspect of ASAL is absolutely essential for developing ASAL transgenic plants. METHODOLOGY/PRINCIPAL FINDINGS: Following the guidelines framed by the Food and Agriculture Organization/World Health Organization, the source of the gene, its sequence homology with potent allergens, clinical tests on mammalian systems, and the pepsin resistance and thermostability of the protein were considered to address the issue. No significant homology to the ASAL sequence was detected when compared to known allergenic proteins. The ELISA of blood sera collected from known allergy patients also failed to show significant evidence of cross-reactivity. In vitro and in vivo assays both indicated the digestibility of ASAL in the presence of pepsin in a minimum time period. CONCLUSIONS/SIGNIFICANCE: With these experiments, we concluded that ASAL does not possess any apparent features of an allergen. This is the first report regarding the monitoring of the allergenicity of any mannose-binding monocot lectin having insecticidal efficacy against hemipteran insects.
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spelling pubmed-32180092011-11-21 Allergenicity Assessment of Allium sativum Leaf Agglutinin, a Potential Candidate Protein for Developing Sap Sucking Insect Resistant Food Crops Mondal, Hossain Ali Chakraborti, Dipankar Majumder, Pralay Roy, Pampa Roy, Amit Bhattacharya, Swati Gupta Das, Sampa PLoS One Research Article BACKGROUND: Mannose-binding Allium sativum leaf agglutinin (ASAL) is highly antinutritional and toxic to various phloem-feeding hemipteran insects. ASAL has been expressed in a number of agriculturally important crops to develop resistance against those insects. Awareness of the safety aspect of ASAL is absolutely essential for developing ASAL transgenic plants. METHODOLOGY/PRINCIPAL FINDINGS: Following the guidelines framed by the Food and Agriculture Organization/World Health Organization, the source of the gene, its sequence homology with potent allergens, clinical tests on mammalian systems, and the pepsin resistance and thermostability of the protein were considered to address the issue. No significant homology to the ASAL sequence was detected when compared to known allergenic proteins. The ELISA of blood sera collected from known allergy patients also failed to show significant evidence of cross-reactivity. In vitro and in vivo assays both indicated the digestibility of ASAL in the presence of pepsin in a minimum time period. CONCLUSIONS/SIGNIFICANCE: With these experiments, we concluded that ASAL does not possess any apparent features of an allergen. This is the first report regarding the monitoring of the allergenicity of any mannose-binding monocot lectin having insecticidal efficacy against hemipteran insects. Public Library of Science 2011-11-16 /pmc/articles/PMC3218009/ /pubmed/22110739 http://dx.doi.org/10.1371/journal.pone.0027716 Text en Mondal et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mondal, Hossain Ali
Chakraborti, Dipankar
Majumder, Pralay
Roy, Pampa
Roy, Amit
Bhattacharya, Swati Gupta
Das, Sampa
Allergenicity Assessment of Allium sativum Leaf Agglutinin, a Potential Candidate Protein for Developing Sap Sucking Insect Resistant Food Crops
title Allergenicity Assessment of Allium sativum Leaf Agglutinin, a Potential Candidate Protein for Developing Sap Sucking Insect Resistant Food Crops
title_full Allergenicity Assessment of Allium sativum Leaf Agglutinin, a Potential Candidate Protein for Developing Sap Sucking Insect Resistant Food Crops
title_fullStr Allergenicity Assessment of Allium sativum Leaf Agglutinin, a Potential Candidate Protein for Developing Sap Sucking Insect Resistant Food Crops
title_full_unstemmed Allergenicity Assessment of Allium sativum Leaf Agglutinin, a Potential Candidate Protein for Developing Sap Sucking Insect Resistant Food Crops
title_short Allergenicity Assessment of Allium sativum Leaf Agglutinin, a Potential Candidate Protein for Developing Sap Sucking Insect Resistant Food Crops
title_sort allergenicity assessment of allium sativum leaf agglutinin, a potential candidate protein for developing sap sucking insect resistant food crops
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3218009/
https://www.ncbi.nlm.nih.gov/pubmed/22110739
http://dx.doi.org/10.1371/journal.pone.0027716
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