Cargando…

High prevalence of HPV in non-cervical sites of women with abnormal cervical cytology

BACKGROUND: Human papillomaviruses (HPV) are causally associated with ano-genital and a subset of head and neck cancers. Rising incidence of HPV+ anal cancers and head and neck cancers have now been demonstrated in the developed world over the last decade. The majority of published data on HPV preva...

Descripción completa

Detalles Bibliográficos
Autores principales: Crawford, Robin, Grignon, Anne-Laure, Kitson, Sarah, Winder, David M, Ball, Siolian LR, Vaughan, Katie, Stanley, Margaret A, Sterling, Jane C, Goon, Peter KC
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3218103/
https://www.ncbi.nlm.nih.gov/pubmed/22047498
http://dx.doi.org/10.1186/1471-2407-11-473
_version_ 1782216669630824448
author Crawford, Robin
Grignon, Anne-Laure
Kitson, Sarah
Winder, David M
Ball, Siolian LR
Vaughan, Katie
Stanley, Margaret A
Sterling, Jane C
Goon, Peter KC
author_facet Crawford, Robin
Grignon, Anne-Laure
Kitson, Sarah
Winder, David M
Ball, Siolian LR
Vaughan, Katie
Stanley, Margaret A
Sterling, Jane C
Goon, Peter KC
author_sort Crawford, Robin
collection PubMed
description BACKGROUND: Human papillomaviruses (HPV) are causally associated with ano-genital and a subset of head and neck cancers. Rising incidence of HPV+ anal cancers and head and neck cancers have now been demonstrated in the developed world over the last decade. The majority of published data on HPV prevalence at the anal and oro-pharyngeal sites are from studies of higher-risk populations. There is a paucity of data on the prevalence of HPV at non-cervical sites in lower risk, non-HIV+ women and this study was designed to provide initial pilot data on a population of women recalled for colposcopy as part of the UK cervical screening programme. METHODS: 100 non-HIV+ women with abnormal cervical cytology, attending clinic for colposcopic examination were recruited. Swabs from the oro-pharyngeal, anal and cervical sites were taken and DNA extracted. HPV detection and genotyping were performed using a standardised, commercially available PCR-line blot assay, which is used to genotype 37 HPV subtypes known to infect the ano-genital and oro-pharyngeal areas. Strict sampling and laboratory precautions were taken to prevent cross-contamination. RESULTS: There was a very high prevalence of HPV infection at all three sites: 96.0%, 91.4% and 92.4% at the cervix, anus and oro-pharynx, respectively. Multiple HPV subtype infections were dominant at all 3 mucosal sites. At least one or more HR genotype was present at both the cervix/anus in 39/52 (75.0%) patients; both the cervix/oro-pharynx in 48/56 (85.7%) patients; and both the anus/oro-pharynx in 39/52 (75.0%) patients. HPV 16 infection was highly dominant across all mucosal sites, with over a 2-fold increase over the next most prevalent subtype (HPV 31). CONCLUSIONS: Women with abnormal smears have widespread infection with high-risk HPV at the cervical, anal and oro-pharyngeal mucosal sites and may represent a higher risk population for HPV disease in the future.
format Online
Article
Text
id pubmed-3218103
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-32181032011-11-17 High prevalence of HPV in non-cervical sites of women with abnormal cervical cytology Crawford, Robin Grignon, Anne-Laure Kitson, Sarah Winder, David M Ball, Siolian LR Vaughan, Katie Stanley, Margaret A Sterling, Jane C Goon, Peter KC BMC Cancer Research Article BACKGROUND: Human papillomaviruses (HPV) are causally associated with ano-genital and a subset of head and neck cancers. Rising incidence of HPV+ anal cancers and head and neck cancers have now been demonstrated in the developed world over the last decade. The majority of published data on HPV prevalence at the anal and oro-pharyngeal sites are from studies of higher-risk populations. There is a paucity of data on the prevalence of HPV at non-cervical sites in lower risk, non-HIV+ women and this study was designed to provide initial pilot data on a population of women recalled for colposcopy as part of the UK cervical screening programme. METHODS: 100 non-HIV+ women with abnormal cervical cytology, attending clinic for colposcopic examination were recruited. Swabs from the oro-pharyngeal, anal and cervical sites were taken and DNA extracted. HPV detection and genotyping were performed using a standardised, commercially available PCR-line blot assay, which is used to genotype 37 HPV subtypes known to infect the ano-genital and oro-pharyngeal areas. Strict sampling and laboratory precautions were taken to prevent cross-contamination. RESULTS: There was a very high prevalence of HPV infection at all three sites: 96.0%, 91.4% and 92.4% at the cervix, anus and oro-pharynx, respectively. Multiple HPV subtype infections were dominant at all 3 mucosal sites. At least one or more HR genotype was present at both the cervix/anus in 39/52 (75.0%) patients; both the cervix/oro-pharynx in 48/56 (85.7%) patients; and both the anus/oro-pharynx in 39/52 (75.0%) patients. HPV 16 infection was highly dominant across all mucosal sites, with over a 2-fold increase over the next most prevalent subtype (HPV 31). CONCLUSIONS: Women with abnormal smears have widespread infection with high-risk HPV at the cervical, anal and oro-pharyngeal mucosal sites and may represent a higher risk population for HPV disease in the future. BioMed Central 2011-11-02 /pmc/articles/PMC3218103/ /pubmed/22047498 http://dx.doi.org/10.1186/1471-2407-11-473 Text en Copyright ©2011 Crawford et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Crawford, Robin
Grignon, Anne-Laure
Kitson, Sarah
Winder, David M
Ball, Siolian LR
Vaughan, Katie
Stanley, Margaret A
Sterling, Jane C
Goon, Peter KC
High prevalence of HPV in non-cervical sites of women with abnormal cervical cytology
title High prevalence of HPV in non-cervical sites of women with abnormal cervical cytology
title_full High prevalence of HPV in non-cervical sites of women with abnormal cervical cytology
title_fullStr High prevalence of HPV in non-cervical sites of women with abnormal cervical cytology
title_full_unstemmed High prevalence of HPV in non-cervical sites of women with abnormal cervical cytology
title_short High prevalence of HPV in non-cervical sites of women with abnormal cervical cytology
title_sort high prevalence of hpv in non-cervical sites of women with abnormal cervical cytology
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3218103/
https://www.ncbi.nlm.nih.gov/pubmed/22047498
http://dx.doi.org/10.1186/1471-2407-11-473
work_keys_str_mv AT crawfordrobin highprevalenceofhpvinnoncervicalsitesofwomenwithabnormalcervicalcytology
AT grignonannelaure highprevalenceofhpvinnoncervicalsitesofwomenwithabnormalcervicalcytology
AT kitsonsarah highprevalenceofhpvinnoncervicalsitesofwomenwithabnormalcervicalcytology
AT winderdavidm highprevalenceofhpvinnoncervicalsitesofwomenwithabnormalcervicalcytology
AT ballsiolianlr highprevalenceofhpvinnoncervicalsitesofwomenwithabnormalcervicalcytology
AT vaughankatie highprevalenceofhpvinnoncervicalsitesofwomenwithabnormalcervicalcytology
AT stanleymargareta highprevalenceofhpvinnoncervicalsitesofwomenwithabnormalcervicalcytology
AT sterlingjanec highprevalenceofhpvinnoncervicalsitesofwomenwithabnormalcervicalcytology
AT goonpeterkc highprevalenceofhpvinnoncervicalsitesofwomenwithabnormalcervicalcytology