N(6)-Methyladenosine in Nuclear RNA is a Major Substrate of the Obesity-Associated FTO

We report here that FTO (fat mass and obesity-associated protein) exhibits efficient oxidative demethylation activity of abundant N(6)-methyladenosine (m(6)A) residues in RNA in vitro. FTO knockdown with siRNA led to an increased level of m(6)A in mRNA, whereas overexpression of FTO resulted in a de...

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Autores principales: Jia, Guifang, Fu, Ye, Zhao, Xu, Dai, Qing, Zheng, Guanqun, Yang, Ying, Yi, Chengqi, Lindahl, Tomas, Pan, Tao, Yang, Yun-Gui, He, Chuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3218240/
https://www.ncbi.nlm.nih.gov/pubmed/22002720
http://dx.doi.org/10.1038/nchembio.687
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author Jia, Guifang
Fu, Ye
Zhao, Xu
Dai, Qing
Zheng, Guanqun
Yang, Ying
Yi, Chengqi
Lindahl, Tomas
Pan, Tao
Yang, Yun-Gui
He, Chuan
author_facet Jia, Guifang
Fu, Ye
Zhao, Xu
Dai, Qing
Zheng, Guanqun
Yang, Ying
Yi, Chengqi
Lindahl, Tomas
Pan, Tao
Yang, Yun-Gui
He, Chuan
author_sort Jia, Guifang
collection PubMed
description We report here that FTO (fat mass and obesity-associated protein) exhibits efficient oxidative demethylation activity of abundant N(6)-methyladenosine (m(6)A) residues in RNA in vitro. FTO knockdown with siRNA led to an increased level of m(6)A in mRNA, whereas overexpression of FTO resulted in a decreased level of m(6)A in human cells. We further show that FTO partially colocalizes with nuclear speckles, supporting m(6)A in nuclear RNA as a physiological substrate of FTO.
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spelling pubmed-32182402012-06-01 N(6)-Methyladenosine in Nuclear RNA is a Major Substrate of the Obesity-Associated FTO Jia, Guifang Fu, Ye Zhao, Xu Dai, Qing Zheng, Guanqun Yang, Ying Yi, Chengqi Lindahl, Tomas Pan, Tao Yang, Yun-Gui He, Chuan Nat Chem Biol Article We report here that FTO (fat mass and obesity-associated protein) exhibits efficient oxidative demethylation activity of abundant N(6)-methyladenosine (m(6)A) residues in RNA in vitro. FTO knockdown with siRNA led to an increased level of m(6)A in mRNA, whereas overexpression of FTO resulted in a decreased level of m(6)A in human cells. We further show that FTO partially colocalizes with nuclear speckles, supporting m(6)A in nuclear RNA as a physiological substrate of FTO. 2011-10-16 /pmc/articles/PMC3218240/ /pubmed/22002720 http://dx.doi.org/10.1038/nchembio.687 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Jia, Guifang
Fu, Ye
Zhao, Xu
Dai, Qing
Zheng, Guanqun
Yang, Ying
Yi, Chengqi
Lindahl, Tomas
Pan, Tao
Yang, Yun-Gui
He, Chuan
N(6)-Methyladenosine in Nuclear RNA is a Major Substrate of the Obesity-Associated FTO
title N(6)-Methyladenosine in Nuclear RNA is a Major Substrate of the Obesity-Associated FTO
title_full N(6)-Methyladenosine in Nuclear RNA is a Major Substrate of the Obesity-Associated FTO
title_fullStr N(6)-Methyladenosine in Nuclear RNA is a Major Substrate of the Obesity-Associated FTO
title_full_unstemmed N(6)-Methyladenosine in Nuclear RNA is a Major Substrate of the Obesity-Associated FTO
title_short N(6)-Methyladenosine in Nuclear RNA is a Major Substrate of the Obesity-Associated FTO
title_sort n(6)-methyladenosine in nuclear rna is a major substrate of the obesity-associated fto
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3218240/
https://www.ncbi.nlm.nih.gov/pubmed/22002720
http://dx.doi.org/10.1038/nchembio.687
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