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DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes

BACKGROUND: In the present study, we investigated whether DJ-1 could serve as a biomarker for assessing the biocompatibility of multiwalled carbon nanotubes (MWCNTs), using the highly purified carbon nanotube, HTT2800. METHODS: Using Western blot analysis, we determined DJ-1 protein levels in two di...

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Autores principales: Haniu, Hisao, Tsukahara, Tamotsu, Matsuda, Yoshikazu, Usui, Yuki, Aoki, Kaoru, Shimizu, Masayuki, Ogihara, Nobuhide, Hara, Kazuo, Takanashi, Seiji, Okamoto, Masanori, Ishigaki, Norio, Nakamura, Koichi, Kato, Hiroyuki, Saito, Naoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3218582/
https://www.ncbi.nlm.nih.gov/pubmed/22114499
http://dx.doi.org/10.2147/IJN.S25471
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author Haniu, Hisao
Tsukahara, Tamotsu
Matsuda, Yoshikazu
Usui, Yuki
Aoki, Kaoru
Shimizu, Masayuki
Ogihara, Nobuhide
Hara, Kazuo
Takanashi, Seiji
Okamoto, Masanori
Ishigaki, Norio
Nakamura, Koichi
Kato, Hiroyuki
Saito, Naoto
author_facet Haniu, Hisao
Tsukahara, Tamotsu
Matsuda, Yoshikazu
Usui, Yuki
Aoki, Kaoru
Shimizu, Masayuki
Ogihara, Nobuhide
Hara, Kazuo
Takanashi, Seiji
Okamoto, Masanori
Ishigaki, Norio
Nakamura, Koichi
Kato, Hiroyuki
Saito, Naoto
author_sort Haniu, Hisao
collection PubMed
description BACKGROUND: In the present study, we investigated whether DJ-1 could serve as a biomarker for assessing the biocompatibility of multiwalled carbon nanotubes (MWCNTs), using the highly purified carbon nanotube, HTT2800. METHODS: Using Western blot analysis, we determined DJ-1 protein levels in two different types of cells (one capable and the other incapable of HTT2800 endocytosis). Using quantitative real-time polymerase chain reaction, we also investigated the ability of purified nanotubes to alter DJ-1 mRNA levels. RESULTS: We demonstrated that the DJ-1 protein concentration was reduced, regardless of the cytotoxic activity of intracellular HTT2800. Furthermore, HTT2800 decreased the DJ-1 mRNA levels in a dose-dependent manner. This decrease in DJ-1 mRNA levels was not observed in the case of Sumi black or cup-stacked carbon nanotubes. CONCLUSION: These data indicate that modification of DJ-1 expression is caused by the cell response to MWCNTs. We conclude that DJ-1 is a promising candidate biomarker for the development of biocompatible MWCNTs.
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spelling pubmed-32185822011-11-23 DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes Haniu, Hisao Tsukahara, Tamotsu Matsuda, Yoshikazu Usui, Yuki Aoki, Kaoru Shimizu, Masayuki Ogihara, Nobuhide Hara, Kazuo Takanashi, Seiji Okamoto, Masanori Ishigaki, Norio Nakamura, Koichi Kato, Hiroyuki Saito, Naoto Int J Nanomedicine Original Research BACKGROUND: In the present study, we investigated whether DJ-1 could serve as a biomarker for assessing the biocompatibility of multiwalled carbon nanotubes (MWCNTs), using the highly purified carbon nanotube, HTT2800. METHODS: Using Western blot analysis, we determined DJ-1 protein levels in two different types of cells (one capable and the other incapable of HTT2800 endocytosis). Using quantitative real-time polymerase chain reaction, we also investigated the ability of purified nanotubes to alter DJ-1 mRNA levels. RESULTS: We demonstrated that the DJ-1 protein concentration was reduced, regardless of the cytotoxic activity of intracellular HTT2800. Furthermore, HTT2800 decreased the DJ-1 mRNA levels in a dose-dependent manner. This decrease in DJ-1 mRNA levels was not observed in the case of Sumi black or cup-stacked carbon nanotubes. CONCLUSION: These data indicate that modification of DJ-1 expression is caused by the cell response to MWCNTs. We conclude that DJ-1 is a promising candidate biomarker for the development of biocompatible MWCNTs. Dove Medical Press 2011 2011-11-04 /pmc/articles/PMC3218582/ /pubmed/22114499 http://dx.doi.org/10.2147/IJN.S25471 Text en © 2011 Haniu et al, publisher and licensee Dove Medical Press Ltd This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Haniu, Hisao
Tsukahara, Tamotsu
Matsuda, Yoshikazu
Usui, Yuki
Aoki, Kaoru
Shimizu, Masayuki
Ogihara, Nobuhide
Hara, Kazuo
Takanashi, Seiji
Okamoto, Masanori
Ishigaki, Norio
Nakamura, Koichi
Kato, Hiroyuki
Saito, Naoto
DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes
title DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes
title_full DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes
title_fullStr DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes
title_full_unstemmed DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes
title_short DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes
title_sort dj-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3218582/
https://www.ncbi.nlm.nih.gov/pubmed/22114499
http://dx.doi.org/10.2147/IJN.S25471
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