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DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes
BACKGROUND: In the present study, we investigated whether DJ-1 could serve as a biomarker for assessing the biocompatibility of multiwalled carbon nanotubes (MWCNTs), using the highly purified carbon nanotube, HTT2800. METHODS: Using Western blot analysis, we determined DJ-1 protein levels in two di...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3218582/ https://www.ncbi.nlm.nih.gov/pubmed/22114499 http://dx.doi.org/10.2147/IJN.S25471 |
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author | Haniu, Hisao Tsukahara, Tamotsu Matsuda, Yoshikazu Usui, Yuki Aoki, Kaoru Shimizu, Masayuki Ogihara, Nobuhide Hara, Kazuo Takanashi, Seiji Okamoto, Masanori Ishigaki, Norio Nakamura, Koichi Kato, Hiroyuki Saito, Naoto |
author_facet | Haniu, Hisao Tsukahara, Tamotsu Matsuda, Yoshikazu Usui, Yuki Aoki, Kaoru Shimizu, Masayuki Ogihara, Nobuhide Hara, Kazuo Takanashi, Seiji Okamoto, Masanori Ishigaki, Norio Nakamura, Koichi Kato, Hiroyuki Saito, Naoto |
author_sort | Haniu, Hisao |
collection | PubMed |
description | BACKGROUND: In the present study, we investigated whether DJ-1 could serve as a biomarker for assessing the biocompatibility of multiwalled carbon nanotubes (MWCNTs), using the highly purified carbon nanotube, HTT2800. METHODS: Using Western blot analysis, we determined DJ-1 protein levels in two different types of cells (one capable and the other incapable of HTT2800 endocytosis). Using quantitative real-time polymerase chain reaction, we also investigated the ability of purified nanotubes to alter DJ-1 mRNA levels. RESULTS: We demonstrated that the DJ-1 protein concentration was reduced, regardless of the cytotoxic activity of intracellular HTT2800. Furthermore, HTT2800 decreased the DJ-1 mRNA levels in a dose-dependent manner. This decrease in DJ-1 mRNA levels was not observed in the case of Sumi black or cup-stacked carbon nanotubes. CONCLUSION: These data indicate that modification of DJ-1 expression is caused by the cell response to MWCNTs. We conclude that DJ-1 is a promising candidate biomarker for the development of biocompatible MWCNTs. |
format | Online Article Text |
id | pubmed-3218582 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-32185822011-11-23 DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes Haniu, Hisao Tsukahara, Tamotsu Matsuda, Yoshikazu Usui, Yuki Aoki, Kaoru Shimizu, Masayuki Ogihara, Nobuhide Hara, Kazuo Takanashi, Seiji Okamoto, Masanori Ishigaki, Norio Nakamura, Koichi Kato, Hiroyuki Saito, Naoto Int J Nanomedicine Original Research BACKGROUND: In the present study, we investigated whether DJ-1 could serve as a biomarker for assessing the biocompatibility of multiwalled carbon nanotubes (MWCNTs), using the highly purified carbon nanotube, HTT2800. METHODS: Using Western blot analysis, we determined DJ-1 protein levels in two different types of cells (one capable and the other incapable of HTT2800 endocytosis). Using quantitative real-time polymerase chain reaction, we also investigated the ability of purified nanotubes to alter DJ-1 mRNA levels. RESULTS: We demonstrated that the DJ-1 protein concentration was reduced, regardless of the cytotoxic activity of intracellular HTT2800. Furthermore, HTT2800 decreased the DJ-1 mRNA levels in a dose-dependent manner. This decrease in DJ-1 mRNA levels was not observed in the case of Sumi black or cup-stacked carbon nanotubes. CONCLUSION: These data indicate that modification of DJ-1 expression is caused by the cell response to MWCNTs. We conclude that DJ-1 is a promising candidate biomarker for the development of biocompatible MWCNTs. Dove Medical Press 2011 2011-11-04 /pmc/articles/PMC3218582/ /pubmed/22114499 http://dx.doi.org/10.2147/IJN.S25471 Text en © 2011 Haniu et al, publisher and licensee Dove Medical Press Ltd This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Haniu, Hisao Tsukahara, Tamotsu Matsuda, Yoshikazu Usui, Yuki Aoki, Kaoru Shimizu, Masayuki Ogihara, Nobuhide Hara, Kazuo Takanashi, Seiji Okamoto, Masanori Ishigaki, Norio Nakamura, Koichi Kato, Hiroyuki Saito, Naoto DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes |
title | DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes |
title_full | DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes |
title_fullStr | DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes |
title_full_unstemmed | DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes |
title_short | DJ-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes |
title_sort | dj-1 as a potential biomarker for the development of biocompatible multiwalled carbon nanotubes |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3218582/ https://www.ncbi.nlm.nih.gov/pubmed/22114499 http://dx.doi.org/10.2147/IJN.S25471 |
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