Cargando…

Small RNA sequencing reveals miR-642a-3p as a novel adipocyte-specific microRNA and miR-30 as a key regulator of human adipogenesis

BACKGROUND: In severe obesity, as well as in normal development, the growth of adipose tissue is the result of an increase in adipocyte size and numbers, which is underlain by the stimulation of adipogenic differentiation of precursor cells. A better knowledge of the pathways that regulate adipogene...

Descripción completa

Detalles Bibliográficos
Autores principales: Zaragosi, Laure-Emmanuelle, Wdziekonski, Brigitte, Brigand, Kevin Le, Villageois, Phi, Mari, Bernard, Waldmann, Rainer, Dani, Christian, Barbry, Pascal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3218826/
https://www.ncbi.nlm.nih.gov/pubmed/21767385
http://dx.doi.org/10.1186/gb-2011-12-7-r64
_version_ 1782216736475447296
author Zaragosi, Laure-Emmanuelle
Wdziekonski, Brigitte
Brigand, Kevin Le
Villageois, Phi
Mari, Bernard
Waldmann, Rainer
Dani, Christian
Barbry, Pascal
author_facet Zaragosi, Laure-Emmanuelle
Wdziekonski, Brigitte
Brigand, Kevin Le
Villageois, Phi
Mari, Bernard
Waldmann, Rainer
Dani, Christian
Barbry, Pascal
author_sort Zaragosi, Laure-Emmanuelle
collection PubMed
description BACKGROUND: In severe obesity, as well as in normal development, the growth of adipose tissue is the result of an increase in adipocyte size and numbers, which is underlain by the stimulation of adipogenic differentiation of precursor cells. A better knowledge of the pathways that regulate adipogenesis is therefore essential for an improved understanding of adipose tissue expansion. As microRNAs (miRNAs) have a critical role in many differentiation processes, our study aimed to identify the role of miRNA-mediated gene silencing in the regulation of adipogenic differentiation. RESULTS: We used deep sequencing to identify small RNAs that are differentially expressed during adipogenesis of adipose tissue-derived stem cells. This approach revealed the un-annotated miR-642a-3p as a highly adipocyte-specific miRNA. We then focused our study on the miR-30 family, which was also up-regulated during adipogenic differentiation and for which the role in adipogenesis had not yet been elucidated. Inhibition of the miR-30 family blocked adipogenesis, whilst over-expression of miR-30a and miR-30d stimulated this process. We additionally showed that both miR-30a and miR-30d target the transcription factor RUNX2, and stimulate adipogenesis via the modulation of this major regulator of osteogenesis. CONCLUSIONS: Overall, our data suggest that the miR-30 family plays a central role in adipocyte development. Moreover, as adipose tissue-derived stem cells can differentiate into either adipocytes or osteoblasts, the down-regulation of the osteogenesis regulator RUNX2 represents a plausible mechanism by which miR-30 miRNAs may contribute to adipogenic differentiation of adipose tissue-derived stem cells.
format Online
Article
Text
id pubmed-3218826
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-32188262011-11-18 Small RNA sequencing reveals miR-642a-3p as a novel adipocyte-specific microRNA and miR-30 as a key regulator of human adipogenesis Zaragosi, Laure-Emmanuelle Wdziekonski, Brigitte Brigand, Kevin Le Villageois, Phi Mari, Bernard Waldmann, Rainer Dani, Christian Barbry, Pascal Genome Biol Research BACKGROUND: In severe obesity, as well as in normal development, the growth of adipose tissue is the result of an increase in adipocyte size and numbers, which is underlain by the stimulation of adipogenic differentiation of precursor cells. A better knowledge of the pathways that regulate adipogenesis is therefore essential for an improved understanding of adipose tissue expansion. As microRNAs (miRNAs) have a critical role in many differentiation processes, our study aimed to identify the role of miRNA-mediated gene silencing in the regulation of adipogenic differentiation. RESULTS: We used deep sequencing to identify small RNAs that are differentially expressed during adipogenesis of adipose tissue-derived stem cells. This approach revealed the un-annotated miR-642a-3p as a highly adipocyte-specific miRNA. We then focused our study on the miR-30 family, which was also up-regulated during adipogenic differentiation and for which the role in adipogenesis had not yet been elucidated. Inhibition of the miR-30 family blocked adipogenesis, whilst over-expression of miR-30a and miR-30d stimulated this process. We additionally showed that both miR-30a and miR-30d target the transcription factor RUNX2, and stimulate adipogenesis via the modulation of this major regulator of osteogenesis. CONCLUSIONS: Overall, our data suggest that the miR-30 family plays a central role in adipocyte development. Moreover, as adipose tissue-derived stem cells can differentiate into either adipocytes or osteoblasts, the down-regulation of the osteogenesis regulator RUNX2 represents a plausible mechanism by which miR-30 miRNAs may contribute to adipogenic differentiation of adipose tissue-derived stem cells. BioMed Central 2011 2011-07-18 /pmc/articles/PMC3218826/ /pubmed/21767385 http://dx.doi.org/10.1186/gb-2011-12-7-r64 Text en Copyright ©2011 Zaragosi et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Zaragosi, Laure-Emmanuelle
Wdziekonski, Brigitte
Brigand, Kevin Le
Villageois, Phi
Mari, Bernard
Waldmann, Rainer
Dani, Christian
Barbry, Pascal
Small RNA sequencing reveals miR-642a-3p as a novel adipocyte-specific microRNA and miR-30 as a key regulator of human adipogenesis
title Small RNA sequencing reveals miR-642a-3p as a novel adipocyte-specific microRNA and miR-30 as a key regulator of human adipogenesis
title_full Small RNA sequencing reveals miR-642a-3p as a novel adipocyte-specific microRNA and miR-30 as a key regulator of human adipogenesis
title_fullStr Small RNA sequencing reveals miR-642a-3p as a novel adipocyte-specific microRNA and miR-30 as a key regulator of human adipogenesis
title_full_unstemmed Small RNA sequencing reveals miR-642a-3p as a novel adipocyte-specific microRNA and miR-30 as a key regulator of human adipogenesis
title_short Small RNA sequencing reveals miR-642a-3p as a novel adipocyte-specific microRNA and miR-30 as a key regulator of human adipogenesis
title_sort small rna sequencing reveals mir-642a-3p as a novel adipocyte-specific microrna and mir-30 as a key regulator of human adipogenesis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3218826/
https://www.ncbi.nlm.nih.gov/pubmed/21767385
http://dx.doi.org/10.1186/gb-2011-12-7-r64
work_keys_str_mv AT zaragosilaureemmanuelle smallrnasequencingrevealsmir642a3pasanoveladipocytespecificmicrornaandmir30asakeyregulatorofhumanadipogenesis
AT wdziekonskibrigitte smallrnasequencingrevealsmir642a3pasanoveladipocytespecificmicrornaandmir30asakeyregulatorofhumanadipogenesis
AT brigandkevinle smallrnasequencingrevealsmir642a3pasanoveladipocytespecificmicrornaandmir30asakeyregulatorofhumanadipogenesis
AT villageoisphi smallrnasequencingrevealsmir642a3pasanoveladipocytespecificmicrornaandmir30asakeyregulatorofhumanadipogenesis
AT maribernard smallrnasequencingrevealsmir642a3pasanoveladipocytespecificmicrornaandmir30asakeyregulatorofhumanadipogenesis
AT waldmannrainer smallrnasequencingrevealsmir642a3pasanoveladipocytespecificmicrornaandmir30asakeyregulatorofhumanadipogenesis
AT danichristian smallrnasequencingrevealsmir642a3pasanoveladipocytespecificmicrornaandmir30asakeyregulatorofhumanadipogenesis
AT barbrypascal smallrnasequencingrevealsmir642a3pasanoveladipocytespecificmicrornaandmir30asakeyregulatorofhumanadipogenesis