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Analysis of IL-17(+ )cells in facet joints of patients with spondyloarthritis suggests that the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response
INTRODUCTION: In this study, we analysed the number of IL-17(+ )cells in facet joints, in the peripheral blood (PB) and synovial fluid (SF) of spondyloarthritis (SpA) patients and compared these results with those of patients with other rheumatic diseases and controls. METHODS: Immunohistochemical a...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3218910/ https://www.ncbi.nlm.nih.gov/pubmed/21689402 http://dx.doi.org/10.1186/ar3370 |
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author | Appel, Heiner Maier, René Wu, Peihua Scheer, Rebecca Hempfing, Axel Kayser, Ralph Thiel, Andreas Radbruch, Andreas Loddenkemper, Christoph Sieper, Joachim |
author_facet | Appel, Heiner Maier, René Wu, Peihua Scheer, Rebecca Hempfing, Axel Kayser, Ralph Thiel, Andreas Radbruch, Andreas Loddenkemper, Christoph Sieper, Joachim |
author_sort | Appel, Heiner |
collection | PubMed |
description | INTRODUCTION: In this study, we analysed the number of IL-17(+ )cells in facet joints, in the peripheral blood (PB) and synovial fluid (SF) of spondyloarthritis (SpA) patients and compared these results with those of patients with other rheumatic diseases and controls. METHODS: Immunohistochemical analysis of IL-17(+ )cells was performed in facet joints of 33 ankylosing spondylitis (AS) patients and compared with data from 20 osteoarthritis (OA) patients. The frequency of IL-17(+)CD4(+ )T cells in PB and SF of SpA patients (PB n = 30, SF n = 11), rheumatoid arthritis (RA) patients (PB n = 14, SF n = 7), OA patients (PB n = 10) and healthy controls (PB n = 12) was analysed after stimulation with Staphylococcus aureus Enterotoxin B and phorbol 12-myristate 13-acetate/ionomycin and quantified by flow cytometry. RESULTS: In AS facet joints, the frequency of IL-17-secreting cells was significantly higher than in samples obtained from OA patients (P < 0.001), with a slight predominance of IL-17(+ )cells among the mononuclear cells (61.5% ± 14.9%) compared to cells with polysegmental nuclei. Immunofluorescence microscopy revealed that the majority of IL-17(+ )cells were myeloperoxidase-positive (35.84 ± 13.06/high-power field (HPF) and CD15(+ )neutrophils (24.25 ± 10.36/HPF), while CD3(+ )T cells (0.51 ± 0.49/HPF) and AA-1(+ )mast cells (2.28 ± 1.96/HPF) were less often IL-17-positive. The frequency of IL-17(+)CD4(+ )T cells in the PB and SF of SpA patients did not differ significantly compared to RA patients, OA patients or healthy controls. CONCLUSIONS: Our data suggest an important role for IL-17 in the inflammatory processes in AS. However, the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response. |
format | Online Article Text |
id | pubmed-3218910 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-32189102011-11-18 Analysis of IL-17(+ )cells in facet joints of patients with spondyloarthritis suggests that the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response Appel, Heiner Maier, René Wu, Peihua Scheer, Rebecca Hempfing, Axel Kayser, Ralph Thiel, Andreas Radbruch, Andreas Loddenkemper, Christoph Sieper, Joachim Arthritis Res Ther Research Article INTRODUCTION: In this study, we analysed the number of IL-17(+ )cells in facet joints, in the peripheral blood (PB) and synovial fluid (SF) of spondyloarthritis (SpA) patients and compared these results with those of patients with other rheumatic diseases and controls. METHODS: Immunohistochemical analysis of IL-17(+ )cells was performed in facet joints of 33 ankylosing spondylitis (AS) patients and compared with data from 20 osteoarthritis (OA) patients. The frequency of IL-17(+)CD4(+ )T cells in PB and SF of SpA patients (PB n = 30, SF n = 11), rheumatoid arthritis (RA) patients (PB n = 14, SF n = 7), OA patients (PB n = 10) and healthy controls (PB n = 12) was analysed after stimulation with Staphylococcus aureus Enterotoxin B and phorbol 12-myristate 13-acetate/ionomycin and quantified by flow cytometry. RESULTS: In AS facet joints, the frequency of IL-17-secreting cells was significantly higher than in samples obtained from OA patients (P < 0.001), with a slight predominance of IL-17(+ )cells among the mononuclear cells (61.5% ± 14.9%) compared to cells with polysegmental nuclei. Immunofluorescence microscopy revealed that the majority of IL-17(+ )cells were myeloperoxidase-positive (35.84 ± 13.06/high-power field (HPF) and CD15(+ )neutrophils (24.25 ± 10.36/HPF), while CD3(+ )T cells (0.51 ± 0.49/HPF) and AA-1(+ )mast cells (2.28 ± 1.96/HPF) were less often IL-17-positive. The frequency of IL-17(+)CD4(+ )T cells in the PB and SF of SpA patients did not differ significantly compared to RA patients, OA patients or healthy controls. CONCLUSIONS: Our data suggest an important role for IL-17 in the inflammatory processes in AS. However, the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response. BioMed Central 2011 2011-06-20 /pmc/articles/PMC3218910/ /pubmed/21689402 http://dx.doi.org/10.1186/ar3370 Text en Copyright ©2011 Appel et al.; licensee BioMed Central Ltd http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction. |
spellingShingle | Research Article Appel, Heiner Maier, René Wu, Peihua Scheer, Rebecca Hempfing, Axel Kayser, Ralph Thiel, Andreas Radbruch, Andreas Loddenkemper, Christoph Sieper, Joachim Analysis of IL-17(+ )cells in facet joints of patients with spondyloarthritis suggests that the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response |
title | Analysis of IL-17(+ )cells in facet joints of patients with spondyloarthritis suggests that the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response |
title_full | Analysis of IL-17(+ )cells in facet joints of patients with spondyloarthritis suggests that the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response |
title_fullStr | Analysis of IL-17(+ )cells in facet joints of patients with spondyloarthritis suggests that the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response |
title_full_unstemmed | Analysis of IL-17(+ )cells in facet joints of patients with spondyloarthritis suggests that the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response |
title_short | Analysis of IL-17(+ )cells in facet joints of patients with spondyloarthritis suggests that the innate immune pathway might be of greater relevance than the Th17-mediated adaptive immune response |
title_sort | analysis of il-17(+ )cells in facet joints of patients with spondyloarthritis suggests that the innate immune pathway might be of greater relevance than the th17-mediated adaptive immune response |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3218910/ https://www.ncbi.nlm.nih.gov/pubmed/21689402 http://dx.doi.org/10.1186/ar3370 |
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