Invasion of Ureaplasma diversum in bovine spermatozoids

BACKGROUND: Ureaplasma diversum has been associated with infertility in cows. In bulls, this mollicute colonizes the prepuce and distal portion of the urethra and may infect sperm cells. The aim of this study is to analyze in vitro interaction of U. diversum isolates and ATCC strains with bovine spe...

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Autores principales: Buzinhani, Melissa, Yamaguti, Maurício, Oliveira, Rosângela C, Cortez, Beatriz A, Marques, Lucas Miranda, Machado-Santelli, Gláucia M, Assumpção, Mayra EO, Timenetsky, Jorge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3219583/
https://www.ncbi.nlm.nih.gov/pubmed/22032232
http://dx.doi.org/10.1186/1756-0500-4-455
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author Buzinhani, Melissa
Yamaguti, Maurício
Oliveira, Rosângela C
Cortez, Beatriz A
Marques, Lucas Miranda
Machado-Santelli, Gláucia M
Assumpção, Mayra EO
Timenetsky, Jorge
author_facet Buzinhani, Melissa
Yamaguti, Maurício
Oliveira, Rosângela C
Cortez, Beatriz A
Marques, Lucas Miranda
Machado-Santelli, Gláucia M
Assumpção, Mayra EO
Timenetsky, Jorge
author_sort Buzinhani, Melissa
collection PubMed
description BACKGROUND: Ureaplasma diversum has been associated with infertility in cows. In bulls, this mollicute colonizes the prepuce and distal portion of the urethra and may infect sperm cells. The aim of this study is to analyze in vitro interaction of U. diversum isolates and ATCC strains with bovine spermatozoids. The interactions were observed by confocal microscopy and the gentamycin internalization assay. FINDINGS: U. diversum were able to adhere to and invade spermatozoids after 30 min of infection. The gentamicin resistance assay confirmed the intracellularity and survival of U. diversum in bovine spermatozoids. CONCLUSIONS: The intracellular nature of bovine ureaplasma identifies a new difficulty to control the reproductive of these animals.
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spelling pubmed-32195832011-11-18 Invasion of Ureaplasma diversum in bovine spermatozoids Buzinhani, Melissa Yamaguti, Maurício Oliveira, Rosângela C Cortez, Beatriz A Marques, Lucas Miranda Machado-Santelli, Gláucia M Assumpção, Mayra EO Timenetsky, Jorge BMC Res Notes Short Report BACKGROUND: Ureaplasma diversum has been associated with infertility in cows. In bulls, this mollicute colonizes the prepuce and distal portion of the urethra and may infect sperm cells. The aim of this study is to analyze in vitro interaction of U. diversum isolates and ATCC strains with bovine spermatozoids. The interactions were observed by confocal microscopy and the gentamycin internalization assay. FINDINGS: U. diversum were able to adhere to and invade spermatozoids after 30 min of infection. The gentamicin resistance assay confirmed the intracellularity and survival of U. diversum in bovine spermatozoids. CONCLUSIONS: The intracellular nature of bovine ureaplasma identifies a new difficulty to control the reproductive of these animals. BioMed Central 2011-10-27 /pmc/articles/PMC3219583/ /pubmed/22032232 http://dx.doi.org/10.1186/1756-0500-4-455 Text en Copyright ©2010 Timenetsky et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Buzinhani, Melissa
Yamaguti, Maurício
Oliveira, Rosângela C
Cortez, Beatriz A
Marques, Lucas Miranda
Machado-Santelli, Gláucia M
Assumpção, Mayra EO
Timenetsky, Jorge
Invasion of Ureaplasma diversum in bovine spermatozoids
title Invasion of Ureaplasma diversum in bovine spermatozoids
title_full Invasion of Ureaplasma diversum in bovine spermatozoids
title_fullStr Invasion of Ureaplasma diversum in bovine spermatozoids
title_full_unstemmed Invasion of Ureaplasma diversum in bovine spermatozoids
title_short Invasion of Ureaplasma diversum in bovine spermatozoids
title_sort invasion of ureaplasma diversum in bovine spermatozoids
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3219583/
https://www.ncbi.nlm.nih.gov/pubmed/22032232
http://dx.doi.org/10.1186/1756-0500-4-455
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