A mass spectrometric strategy for absolute quantification of Plasmodium falciparum proteins of low abundance

Selected reaction monitoring mass spectrometry has been combined with the use of an isotopically labelled synthetic protein, made up of proteotypic tryptic peptides selected from parasite proteins of interest. This allows, for the first time, absolute quantification of proteins from Plasmodium falci...

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Detalles Bibliográficos
Autores principales: Southworth, Paul M, Hyde, John E, Sims, Paul FG
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3219587/
https://www.ncbi.nlm.nih.gov/pubmed/22027174
http://dx.doi.org/10.1186/1475-2875-10-315
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author Southworth, Paul M
Hyde, John E
Sims, Paul FG
author_facet Southworth, Paul M
Hyde, John E
Sims, Paul FG
author_sort Southworth, Paul M
collection PubMed
description Selected reaction monitoring mass spectrometry has been combined with the use of an isotopically labelled synthetic protein, made up of proteotypic tryptic peptides selected from parasite proteins of interest. This allows, for the first time, absolute quantification of proteins from Plasmodium falciparum. This methodology is demonstrated to be of sufficient sensitivity to quantify, even within whole cell extracts, proteins of low abundance from the folate pathway as well as more abundant "housekeeping" proteins.
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spelling pubmed-32195872011-11-18 A mass spectrometric strategy for absolute quantification of Plasmodium falciparum proteins of low abundance Southworth, Paul M Hyde, John E Sims, Paul FG Malar J Methodology Selected reaction monitoring mass spectrometry has been combined with the use of an isotopically labelled synthetic protein, made up of proteotypic tryptic peptides selected from parasite proteins of interest. This allows, for the first time, absolute quantification of proteins from Plasmodium falciparum. This methodology is demonstrated to be of sufficient sensitivity to quantify, even within whole cell extracts, proteins of low abundance from the folate pathway as well as more abundant "housekeeping" proteins. BioMed Central 2011-10-25 /pmc/articles/PMC3219587/ /pubmed/22027174 http://dx.doi.org/10.1186/1475-2875-10-315 Text en Copyright ©2011 Southworth et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Southworth, Paul M
Hyde, John E
Sims, Paul FG
A mass spectrometric strategy for absolute quantification of Plasmodium falciparum proteins of low abundance
title A mass spectrometric strategy for absolute quantification of Plasmodium falciparum proteins of low abundance
title_full A mass spectrometric strategy for absolute quantification of Plasmodium falciparum proteins of low abundance
title_fullStr A mass spectrometric strategy for absolute quantification of Plasmodium falciparum proteins of low abundance
title_full_unstemmed A mass spectrometric strategy for absolute quantification of Plasmodium falciparum proteins of low abundance
title_short A mass spectrometric strategy for absolute quantification of Plasmodium falciparum proteins of low abundance
title_sort mass spectrometric strategy for absolute quantification of plasmodium falciparum proteins of low abundance
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3219587/
https://www.ncbi.nlm.nih.gov/pubmed/22027174
http://dx.doi.org/10.1186/1475-2875-10-315
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