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Soluble expression, purification and characterization of the full length IS2 Transposase

BACKGROUND: The two-step transposition pathway of insertion sequences of the IS3 family, and several other families, involves first the formation of a branched figure-of-eight (F-8) structure by an asymmetric single strand cleavage at one optional donor end and joining to the flanking host DNA near...

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Detalles Bibliográficos
Autores principales:	Lewis, Leslie A, Astatke, Mekbib, Umekubo, Peter T, Alvi, Shaheen, Saby, Robert, Afrose, Jehan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2011
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3219604/ https://www.ncbi.nlm.nih.gov/pubmed/22032517 http://dx.doi.org/10.1186/1759-8753-2-14

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