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Intestinal Subepithelial Myofibroblasts Support in vitro and in vivo Growth of Human Small Intestinal Epithelium

The intestinal crypt-niche interaction is thought to be essential to the function, maintenance, and proliferation of progenitor stem cells found at the bases of intestinal crypts. These stem cells are constantly renewing the intestinal epithelium by sending differentiated cells from the base of the...

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Autores principales: Lahar, Nicholas, Lei, Nan Ye, Wang, Jiafang, Jabaji, Ziyad, Tung, Stephaine C., Joshi, Vaidehi, Lewis, Michael, Stelzner, Matthias, Martín, Martín G., Dunn, James C. Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3219641/
https://www.ncbi.nlm.nih.gov/pubmed/22125602
http://dx.doi.org/10.1371/journal.pone.0026898
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author Lahar, Nicholas
Lei, Nan Ye
Wang, Jiafang
Jabaji, Ziyad
Tung, Stephaine C.
Joshi, Vaidehi
Lewis, Michael
Stelzner, Matthias
Martín, Martín G.
Dunn, James C. Y.
author_facet Lahar, Nicholas
Lei, Nan Ye
Wang, Jiafang
Jabaji, Ziyad
Tung, Stephaine C.
Joshi, Vaidehi
Lewis, Michael
Stelzner, Matthias
Martín, Martín G.
Dunn, James C. Y.
author_sort Lahar, Nicholas
collection PubMed
description The intestinal crypt-niche interaction is thought to be essential to the function, maintenance, and proliferation of progenitor stem cells found at the bases of intestinal crypts. These stem cells are constantly renewing the intestinal epithelium by sending differentiated cells from the base of the crypts of Lieberkühn to the villus tips where they slough off into the intestinal lumen. The intestinal niche consists of various cell types, extracellular matrix, and growth factors and surrounds the intestinal progenitor cells. There have recently been advances in the understanding of the interactions that regulate the behavior of the intestinal epithelium and there is great interest in methods for isolating and expanding viable intestinal epithelium. However, there is no method to maintain primary human small intestinal epithelium in culture over a prolonged period of time. Similarly no method has been published that describes isolation and support of human intestinal epithelium in an in vivo model. We describe a technique to isolate and maintain human small intestinal epithelium in vitro from surgical specimens. We also describe a novel method to maintain human intestinal epithelium subcutaneously in a mouse model for a prolonged period of time. Our methods require various growth factors and the intimate interaction between intestinal sub-epithelial myofibroblasts (ISEMFs) and the intestinal epithelial cells to support the epithelial in vitro and in vivo growth. Absence of these myofibroblasts precluded successful maintenance of epithelial cell formation and proliferation beyond just a few days, even in the presence of supportive growth factors. We believe that the methods described here can be used to explore the molecular basis of human intestinal stem cell support, maintenance, and growth.
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spelling pubmed-32196412011-11-28 Intestinal Subepithelial Myofibroblasts Support in vitro and in vivo Growth of Human Small Intestinal Epithelium Lahar, Nicholas Lei, Nan Ye Wang, Jiafang Jabaji, Ziyad Tung, Stephaine C. Joshi, Vaidehi Lewis, Michael Stelzner, Matthias Martín, Martín G. Dunn, James C. Y. PLoS One Research Article The intestinal crypt-niche interaction is thought to be essential to the function, maintenance, and proliferation of progenitor stem cells found at the bases of intestinal crypts. These stem cells are constantly renewing the intestinal epithelium by sending differentiated cells from the base of the crypts of Lieberkühn to the villus tips where they slough off into the intestinal lumen. The intestinal niche consists of various cell types, extracellular matrix, and growth factors and surrounds the intestinal progenitor cells. There have recently been advances in the understanding of the interactions that regulate the behavior of the intestinal epithelium and there is great interest in methods for isolating and expanding viable intestinal epithelium. However, there is no method to maintain primary human small intestinal epithelium in culture over a prolonged period of time. Similarly no method has been published that describes isolation and support of human intestinal epithelium in an in vivo model. We describe a technique to isolate and maintain human small intestinal epithelium in vitro from surgical specimens. We also describe a novel method to maintain human intestinal epithelium subcutaneously in a mouse model for a prolonged period of time. Our methods require various growth factors and the intimate interaction between intestinal sub-epithelial myofibroblasts (ISEMFs) and the intestinal epithelial cells to support the epithelial in vitro and in vivo growth. Absence of these myofibroblasts precluded successful maintenance of epithelial cell formation and proliferation beyond just a few days, even in the presence of supportive growth factors. We believe that the methods described here can be used to explore the molecular basis of human intestinal stem cell support, maintenance, and growth. Public Library of Science 2011-11-17 /pmc/articles/PMC3219641/ /pubmed/22125602 http://dx.doi.org/10.1371/journal.pone.0026898 Text en This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Lahar, Nicholas
Lei, Nan Ye
Wang, Jiafang
Jabaji, Ziyad
Tung, Stephaine C.
Joshi, Vaidehi
Lewis, Michael
Stelzner, Matthias
Martín, Martín G.
Dunn, James C. Y.
Intestinal Subepithelial Myofibroblasts Support in vitro and in vivo Growth of Human Small Intestinal Epithelium
title Intestinal Subepithelial Myofibroblasts Support in vitro and in vivo Growth of Human Small Intestinal Epithelium
title_full Intestinal Subepithelial Myofibroblasts Support in vitro and in vivo Growth of Human Small Intestinal Epithelium
title_fullStr Intestinal Subepithelial Myofibroblasts Support in vitro and in vivo Growth of Human Small Intestinal Epithelium
title_full_unstemmed Intestinal Subepithelial Myofibroblasts Support in vitro and in vivo Growth of Human Small Intestinal Epithelium
title_short Intestinal Subepithelial Myofibroblasts Support in vitro and in vivo Growth of Human Small Intestinal Epithelium
title_sort intestinal subepithelial myofibroblasts support in vitro and in vivo growth of human small intestinal epithelium
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3219641/
https://www.ncbi.nlm.nih.gov/pubmed/22125602
http://dx.doi.org/10.1371/journal.pone.0026898
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