No Evidence for XMRV Nucleic Acids, Infectious Virus or Anti-XMRV Antibodies in Canadian Patients with Chronic Fatigue Syndrome

The gammaretroviruses xenotropic murine leukemia virus (MLV)-related virus (XMRV) and MLV have been reported to be more prevalent in plasma and peripheral blood mononuclear cells of chronic fatigue syndrome (CFS) patients than in healthy controls. Here, we report the complex analysis of whole blood...

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Autores principales: Steffen, Imke, Tyrrell, D. Lorne, Stein, Eleanor, Montalvo, Leilani, Lee, Tzong-Hae, Zhou, Yanchen, Lu, Kai, Switzer, William M., Tang, Shaohua, Jia, Hongwei, Hockman, Darren, Santer, Deanna M., Logan, Michael, Landi, Amir, Law, John, Houghton, Michael, Simmons, Graham
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3219700/
https://www.ncbi.nlm.nih.gov/pubmed/22114717
http://dx.doi.org/10.1371/journal.pone.0027870
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author Steffen, Imke
Tyrrell, D. Lorne
Stein, Eleanor
Montalvo, Leilani
Lee, Tzong-Hae
Zhou, Yanchen
Lu, Kai
Switzer, William M.
Tang, Shaohua
Jia, Hongwei
Hockman, Darren
Santer, Deanna M.
Logan, Michael
Landi, Amir
Law, John
Houghton, Michael
Simmons, Graham
author_facet Steffen, Imke
Tyrrell, D. Lorne
Stein, Eleanor
Montalvo, Leilani
Lee, Tzong-Hae
Zhou, Yanchen
Lu, Kai
Switzer, William M.
Tang, Shaohua
Jia, Hongwei
Hockman, Darren
Santer, Deanna M.
Logan, Michael
Landi, Amir
Law, John
Houghton, Michael
Simmons, Graham
author_sort Steffen, Imke
collection PubMed
description The gammaretroviruses xenotropic murine leukemia virus (MLV)-related virus (XMRV) and MLV have been reported to be more prevalent in plasma and peripheral blood mononuclear cells of chronic fatigue syndrome (CFS) patients than in healthy controls. Here, we report the complex analysis of whole blood and plasma samples from 58 CFS patients and 57 controls from Canada for the presence of XMRV/MLV nucleic acids, infectious virus, and XMRV/MLV-specific antibodies. Multiple techniques were employed, including nested and qRT-PCR, cell culture, and immunoblotting. We found no evidence of XMRV or MLV in humans and conclude that CFS is not associated with these gammaretroviruses.
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spelling pubmed-32197002011-11-23 No Evidence for XMRV Nucleic Acids, Infectious Virus or Anti-XMRV Antibodies in Canadian Patients with Chronic Fatigue Syndrome Steffen, Imke Tyrrell, D. Lorne Stein, Eleanor Montalvo, Leilani Lee, Tzong-Hae Zhou, Yanchen Lu, Kai Switzer, William M. Tang, Shaohua Jia, Hongwei Hockman, Darren Santer, Deanna M. Logan, Michael Landi, Amir Law, John Houghton, Michael Simmons, Graham PLoS One Research Article The gammaretroviruses xenotropic murine leukemia virus (MLV)-related virus (XMRV) and MLV have been reported to be more prevalent in plasma and peripheral blood mononuclear cells of chronic fatigue syndrome (CFS) patients than in healthy controls. Here, we report the complex analysis of whole blood and plasma samples from 58 CFS patients and 57 controls from Canada for the presence of XMRV/MLV nucleic acids, infectious virus, and XMRV/MLV-specific antibodies. Multiple techniques were employed, including nested and qRT-PCR, cell culture, and immunoblotting. We found no evidence of XMRV or MLV in humans and conclude that CFS is not associated with these gammaretroviruses. Public Library of Science 2011-11-17 /pmc/articles/PMC3219700/ /pubmed/22114717 http://dx.doi.org/10.1371/journal.pone.0027870 Text en This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Steffen, Imke
Tyrrell, D. Lorne
Stein, Eleanor
Montalvo, Leilani
Lee, Tzong-Hae
Zhou, Yanchen
Lu, Kai
Switzer, William M.
Tang, Shaohua
Jia, Hongwei
Hockman, Darren
Santer, Deanna M.
Logan, Michael
Landi, Amir
Law, John
Houghton, Michael
Simmons, Graham
No Evidence for XMRV Nucleic Acids, Infectious Virus or Anti-XMRV Antibodies in Canadian Patients with Chronic Fatigue Syndrome
title No Evidence for XMRV Nucleic Acids, Infectious Virus or Anti-XMRV Antibodies in Canadian Patients with Chronic Fatigue Syndrome
title_full No Evidence for XMRV Nucleic Acids, Infectious Virus or Anti-XMRV Antibodies in Canadian Patients with Chronic Fatigue Syndrome
title_fullStr No Evidence for XMRV Nucleic Acids, Infectious Virus or Anti-XMRV Antibodies in Canadian Patients with Chronic Fatigue Syndrome
title_full_unstemmed No Evidence for XMRV Nucleic Acids, Infectious Virus or Anti-XMRV Antibodies in Canadian Patients with Chronic Fatigue Syndrome
title_short No Evidence for XMRV Nucleic Acids, Infectious Virus or Anti-XMRV Antibodies in Canadian Patients with Chronic Fatigue Syndrome
title_sort no evidence for xmrv nucleic acids, infectious virus or anti-xmrv antibodies in canadian patients with chronic fatigue syndrome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3219700/
https://www.ncbi.nlm.nih.gov/pubmed/22114717
http://dx.doi.org/10.1371/journal.pone.0027870
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