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Differential expression of CD300a/c on human T(H)1 and T(H)17 cells

BACKGROUND: Human memory CD4(+ )T cells can be either CD300a/c(+ )or CD300a/c(- )and subsequent analyses showed that CD4(+ )effector memory T (T(EM)) cells are mostly CD300a/c(+), whereas CD4(+ )central memory T (T(CM)) cells have similar frequencies of CD300a/c(+ )and CD300a/c(- )cells. RESULTS: Ex...

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Detalles Bibliográficos
Autores principales: Simhadri, Venkateswara R, Mariano, John L, Zhou, Qing, DeBell, Karen E, Borrego, Francisco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3219710/
https://www.ncbi.nlm.nih.gov/pubmed/22046970
http://dx.doi.org/10.1186/1471-2172-12-62
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author Simhadri, Venkateswara R
Mariano, John L
Zhou, Qing
DeBell, Karen E
Borrego, Francisco
author_facet Simhadri, Venkateswara R
Mariano, John L
Zhou, Qing
DeBell, Karen E
Borrego, Francisco
author_sort Simhadri, Venkateswara R
collection PubMed
description BACKGROUND: Human memory CD4(+ )T cells can be either CD300a/c(+ )or CD300a/c(- )and subsequent analyses showed that CD4(+ )effector memory T (T(EM)) cells are mostly CD300a/c(+), whereas CD4(+ )central memory T (T(CM)) cells have similar frequencies of CD300a/c(+ )and CD300a/c(- )cells. RESULTS: Extensive phenotypical and functional characterization showed that in both T(CM )and T(EM )cells, the CD300a/c(+ )subset contained a higher number of T(H)1 (IFN-γ producing) cells. Alternatively, T(H)17 (IL-17a producing) cells tend to be CD300a/c(-), especially in the T(EM )subset. Further characterization of the IL-17a(+ )cells showed that cells that produce only this cytokine are mostly CD300a/c(-), while cells that produce IL-17a in combination with other cytokines, especially IFN-γ, are mostly CD300a/c(+), indicating that the expression of this receptor is associated with cells that produce IFN-γ. Co-ligation of the TCR and CD300a/c in CD4(+ )T cells inhibited Ca(2+ )mobilization evoked by TCR ligation alone and modulated IFN-γ production on T(H)1 polarized cells. CONCLUSION: We conclude that the CD300a/c receptors are differentially expressed on human T(H)1 and T(H)17 cells and that their ligation is capable of modulating TCR mediated signals.
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spelling pubmed-32197102011-11-18 Differential expression of CD300a/c on human T(H)1 and T(H)17 cells Simhadri, Venkateswara R Mariano, John L Zhou, Qing DeBell, Karen E Borrego, Francisco BMC Immunol Research Article BACKGROUND: Human memory CD4(+ )T cells can be either CD300a/c(+ )or CD300a/c(- )and subsequent analyses showed that CD4(+ )effector memory T (T(EM)) cells are mostly CD300a/c(+), whereas CD4(+ )central memory T (T(CM)) cells have similar frequencies of CD300a/c(+ )and CD300a/c(- )cells. RESULTS: Extensive phenotypical and functional characterization showed that in both T(CM )and T(EM )cells, the CD300a/c(+ )subset contained a higher number of T(H)1 (IFN-γ producing) cells. Alternatively, T(H)17 (IL-17a producing) cells tend to be CD300a/c(-), especially in the T(EM )subset. Further characterization of the IL-17a(+ )cells showed that cells that produce only this cytokine are mostly CD300a/c(-), while cells that produce IL-17a in combination with other cytokines, especially IFN-γ, are mostly CD300a/c(+), indicating that the expression of this receptor is associated with cells that produce IFN-γ. Co-ligation of the TCR and CD300a/c in CD4(+ )T cells inhibited Ca(2+ )mobilization evoked by TCR ligation alone and modulated IFN-γ production on T(H)1 polarized cells. CONCLUSION: We conclude that the CD300a/c receptors are differentially expressed on human T(H)1 and T(H)17 cells and that their ligation is capable of modulating TCR mediated signals. BioMed Central 2011-11-02 /pmc/articles/PMC3219710/ /pubmed/22046970 http://dx.doi.org/10.1186/1471-2172-12-62 Text en Copyright ©2011 Simhadri et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Simhadri, Venkateswara R
Mariano, John L
Zhou, Qing
DeBell, Karen E
Borrego, Francisco
Differential expression of CD300a/c on human T(H)1 and T(H)17 cells
title Differential expression of CD300a/c on human T(H)1 and T(H)17 cells
title_full Differential expression of CD300a/c on human T(H)1 and T(H)17 cells
title_fullStr Differential expression of CD300a/c on human T(H)1 and T(H)17 cells
title_full_unstemmed Differential expression of CD300a/c on human T(H)1 and T(H)17 cells
title_short Differential expression of CD300a/c on human T(H)1 and T(H)17 cells
title_sort differential expression of cd300a/c on human t(h)1 and t(h)17 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3219710/
https://www.ncbi.nlm.nih.gov/pubmed/22046970
http://dx.doi.org/10.1186/1471-2172-12-62
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