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Differential expression of CD300a/c on human T(H)1 and T(H)17 cells
BACKGROUND: Human memory CD4(+ )T cells can be either CD300a/c(+ )or CD300a/c(- )and subsequent analyses showed that CD4(+ )effector memory T (T(EM)) cells are mostly CD300a/c(+), whereas CD4(+ )central memory T (T(CM)) cells have similar frequencies of CD300a/c(+ )and CD300a/c(- )cells. RESULTS: Ex...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3219710/ https://www.ncbi.nlm.nih.gov/pubmed/22046970 http://dx.doi.org/10.1186/1471-2172-12-62 |
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author | Simhadri, Venkateswara R Mariano, John L Zhou, Qing DeBell, Karen E Borrego, Francisco |
author_facet | Simhadri, Venkateswara R Mariano, John L Zhou, Qing DeBell, Karen E Borrego, Francisco |
author_sort | Simhadri, Venkateswara R |
collection | PubMed |
description | BACKGROUND: Human memory CD4(+ )T cells can be either CD300a/c(+ )or CD300a/c(- )and subsequent analyses showed that CD4(+ )effector memory T (T(EM)) cells are mostly CD300a/c(+), whereas CD4(+ )central memory T (T(CM)) cells have similar frequencies of CD300a/c(+ )and CD300a/c(- )cells. RESULTS: Extensive phenotypical and functional characterization showed that in both T(CM )and T(EM )cells, the CD300a/c(+ )subset contained a higher number of T(H)1 (IFN-γ producing) cells. Alternatively, T(H)17 (IL-17a producing) cells tend to be CD300a/c(-), especially in the T(EM )subset. Further characterization of the IL-17a(+ )cells showed that cells that produce only this cytokine are mostly CD300a/c(-), while cells that produce IL-17a in combination with other cytokines, especially IFN-γ, are mostly CD300a/c(+), indicating that the expression of this receptor is associated with cells that produce IFN-γ. Co-ligation of the TCR and CD300a/c in CD4(+ )T cells inhibited Ca(2+ )mobilization evoked by TCR ligation alone and modulated IFN-γ production on T(H)1 polarized cells. CONCLUSION: We conclude that the CD300a/c receptors are differentially expressed on human T(H)1 and T(H)17 cells and that their ligation is capable of modulating TCR mediated signals. |
format | Online Article Text |
id | pubmed-3219710 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-32197102011-11-18 Differential expression of CD300a/c on human T(H)1 and T(H)17 cells Simhadri, Venkateswara R Mariano, John L Zhou, Qing DeBell, Karen E Borrego, Francisco BMC Immunol Research Article BACKGROUND: Human memory CD4(+ )T cells can be either CD300a/c(+ )or CD300a/c(- )and subsequent analyses showed that CD4(+ )effector memory T (T(EM)) cells are mostly CD300a/c(+), whereas CD4(+ )central memory T (T(CM)) cells have similar frequencies of CD300a/c(+ )and CD300a/c(- )cells. RESULTS: Extensive phenotypical and functional characterization showed that in both T(CM )and T(EM )cells, the CD300a/c(+ )subset contained a higher number of T(H)1 (IFN-γ producing) cells. Alternatively, T(H)17 (IL-17a producing) cells tend to be CD300a/c(-), especially in the T(EM )subset. Further characterization of the IL-17a(+ )cells showed that cells that produce only this cytokine are mostly CD300a/c(-), while cells that produce IL-17a in combination with other cytokines, especially IFN-γ, are mostly CD300a/c(+), indicating that the expression of this receptor is associated with cells that produce IFN-γ. Co-ligation of the TCR and CD300a/c in CD4(+ )T cells inhibited Ca(2+ )mobilization evoked by TCR ligation alone and modulated IFN-γ production on T(H)1 polarized cells. CONCLUSION: We conclude that the CD300a/c receptors are differentially expressed on human T(H)1 and T(H)17 cells and that their ligation is capable of modulating TCR mediated signals. BioMed Central 2011-11-02 /pmc/articles/PMC3219710/ /pubmed/22046970 http://dx.doi.org/10.1186/1471-2172-12-62 Text en Copyright ©2011 Simhadri et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Simhadri, Venkateswara R Mariano, John L Zhou, Qing DeBell, Karen E Borrego, Francisco Differential expression of CD300a/c on human T(H)1 and T(H)17 cells |
title | Differential expression of CD300a/c on human T(H)1 and T(H)17 cells |
title_full | Differential expression of CD300a/c on human T(H)1 and T(H)17 cells |
title_fullStr | Differential expression of CD300a/c on human T(H)1 and T(H)17 cells |
title_full_unstemmed | Differential expression of CD300a/c on human T(H)1 and T(H)17 cells |
title_short | Differential expression of CD300a/c on human T(H)1 and T(H)17 cells |
title_sort | differential expression of cd300a/c on human t(h)1 and t(h)17 cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3219710/ https://www.ncbi.nlm.nih.gov/pubmed/22046970 http://dx.doi.org/10.1186/1471-2172-12-62 |
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