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TO901317 regulating apolipoprotein M expression mediates via the farnesoid X receptor pathway in Caco-2 cells

BACKGROUND: Apolipoprotein M (apoM) may have potential antiatherosclerotic properties. It has been reported that apoM expression could be regulated by many intracellar and extracellar factors. In the present study we further investigated regulation of apoM expression in Caco-2 cells stimulated by a...

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Autores principales: Zhu, Chunhua, Di, Dongmei, Zhang, Xiaoying, Luo, Guanghua, Wang, Zongchun, Wei, Jiang, Shi, Yuanping, Berggren-Söderlund, Maria, Nilsson-Ehle, Peter, Xu, Ning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3219732/
https://www.ncbi.nlm.nih.gov/pubmed/22054074
http://dx.doi.org/10.1186/1476-511X-10-199
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author Zhu, Chunhua
Di, Dongmei
Zhang, Xiaoying
Luo, Guanghua
Wang, Zongchun
Wei, Jiang
Shi, Yuanping
Berggren-Söderlund, Maria
Nilsson-Ehle, Peter
Xu, Ning
author_facet Zhu, Chunhua
Di, Dongmei
Zhang, Xiaoying
Luo, Guanghua
Wang, Zongchun
Wei, Jiang
Shi, Yuanping
Berggren-Söderlund, Maria
Nilsson-Ehle, Peter
Xu, Ning
author_sort Zhu, Chunhua
collection PubMed
description BACKGROUND: Apolipoprotein M (apoM) may have potential antiatherosclerotic properties. It has been reported that apoM expression could be regulated by many intracellar and extracellar factors. In the present study we further investigated regulation of apoM expression in Caco-2 cells stimulated by a liver X receptor (LXR) agonist, TO901317. MATERIALS AND METHODS: Caco-2 cells were cultured in the presence of either TO901317, farnesoid X receptor (FXR) antagonist guggulsterone or TO901317 together with guggulsterone at different concentrations for 24 hrs. The mRNA levels of ATP-binding cassette transporter A1 (ABCA1), apoA1, apoM, liver receptor homologue-1 (LRH-1) and short heterodimer partner 1 (SHP1) were determined by real-time RT-PCR. RESULTS: When Caco-2 cell cultured with TO901317 alone, the mRNA levels of ABCA1, apoA1, apoM, LRH-1 and SHP1 were significantly increased with dose-dependent manners (p < 0.05), whereas when the cells cultured with guggulsterone alone, the mRNA levels of apoM, SHP1 and LRH-1 (p < 0.05) were strongly inhibited. Moreover, guggulsterone could abolish the TO901317 enhanced mRNA levels of apoA1 apoM, SHP1 and LRH-1. CONCLUSION: The present study demonstrated that LXR agonist TO901317 induced apoM expression in Caco-2 cells might be mediated via the LXR/FXR pathway.
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spelling pubmed-32197322011-11-18 TO901317 regulating apolipoprotein M expression mediates via the farnesoid X receptor pathway in Caco-2 cells Zhu, Chunhua Di, Dongmei Zhang, Xiaoying Luo, Guanghua Wang, Zongchun Wei, Jiang Shi, Yuanping Berggren-Söderlund, Maria Nilsson-Ehle, Peter Xu, Ning Lipids Health Dis Research BACKGROUND: Apolipoprotein M (apoM) may have potential antiatherosclerotic properties. It has been reported that apoM expression could be regulated by many intracellar and extracellar factors. In the present study we further investigated regulation of apoM expression in Caco-2 cells stimulated by a liver X receptor (LXR) agonist, TO901317. MATERIALS AND METHODS: Caco-2 cells were cultured in the presence of either TO901317, farnesoid X receptor (FXR) antagonist guggulsterone or TO901317 together with guggulsterone at different concentrations for 24 hrs. The mRNA levels of ATP-binding cassette transporter A1 (ABCA1), apoA1, apoM, liver receptor homologue-1 (LRH-1) and short heterodimer partner 1 (SHP1) were determined by real-time RT-PCR. RESULTS: When Caco-2 cell cultured with TO901317 alone, the mRNA levels of ABCA1, apoA1, apoM, LRH-1 and SHP1 were significantly increased with dose-dependent manners (p < 0.05), whereas when the cells cultured with guggulsterone alone, the mRNA levels of apoM, SHP1 and LRH-1 (p < 0.05) were strongly inhibited. Moreover, guggulsterone could abolish the TO901317 enhanced mRNA levels of apoA1 apoM, SHP1 and LRH-1. CONCLUSION: The present study demonstrated that LXR agonist TO901317 induced apoM expression in Caco-2 cells might be mediated via the LXR/FXR pathway. BioMed Central 2011-11-04 /pmc/articles/PMC3219732/ /pubmed/22054074 http://dx.doi.org/10.1186/1476-511X-10-199 Text en Copyright ©2011 Zhu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Zhu, Chunhua
Di, Dongmei
Zhang, Xiaoying
Luo, Guanghua
Wang, Zongchun
Wei, Jiang
Shi, Yuanping
Berggren-Söderlund, Maria
Nilsson-Ehle, Peter
Xu, Ning
TO901317 regulating apolipoprotein M expression mediates via the farnesoid X receptor pathway in Caco-2 cells
title TO901317 regulating apolipoprotein M expression mediates via the farnesoid X receptor pathway in Caco-2 cells
title_full TO901317 regulating apolipoprotein M expression mediates via the farnesoid X receptor pathway in Caco-2 cells
title_fullStr TO901317 regulating apolipoprotein M expression mediates via the farnesoid X receptor pathway in Caco-2 cells
title_full_unstemmed TO901317 regulating apolipoprotein M expression mediates via the farnesoid X receptor pathway in Caco-2 cells
title_short TO901317 regulating apolipoprotein M expression mediates via the farnesoid X receptor pathway in Caco-2 cells
title_sort to901317 regulating apolipoprotein m expression mediates via the farnesoid x receptor pathway in caco-2 cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3219732/
https://www.ncbi.nlm.nih.gov/pubmed/22054074
http://dx.doi.org/10.1186/1476-511X-10-199
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