FITC and Ru(phen)(3)(2+ )co-doped silica particles as visualized ratiometric pH indicator

The performance of fluorescein isothiocyanate (FITC) and tris(1, 10-phenanathroline) ruthenium ion (Ru(phen)(3)(2+)) co-doped silica particles as pH indicator was evaluated. The emission intensity ratios of the pH sensitive dye (FITC) and the reference dye (Ru(phen)(3)(2+)) in the particles were dep...

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Detalles Bibliográficos
Autores principales: Xu, Jianquan, Sun, Lei, Li, Jun, Liang, Jinglun, Zhang, Huimao, Yang, Wensheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2011
Materias:
Nano Express
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3220666/
https://www.ncbi.nlm.nih.gov/pubmed/22027093
http://dx.doi.org/10.1186/1556-276X-6-561
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author Xu, Jianquan
Sun, Lei
Li, Jun
Liang, Jinglun
Zhang, Huimao
Yang, Wensheng
author_facet Xu, Jianquan
Sun, Lei
Li, Jun
Liang, Jinglun
Zhang, Huimao
Yang, Wensheng
author_sort Xu, Jianquan
collection PubMed
description The performance of fluorescein isothiocyanate (FITC) and tris(1, 10-phenanathroline) ruthenium ion (Ru(phen)(3)(2+)) co-doped silica particles as pH indicator was evaluated. The emission intensity ratios of the pH sensitive dye (FITC) and the reference dye (Ru(phen)(3)(2+)) in the particles were dependent on pH of the environment. The changes in emission intensity ratios of the two dyes under different pH could be measured under single excitation wavelength and readily visualized by naked eye under a 365-nm UV lamp. In particular, such FITC and Ru(phen)(3)(2+ )co-doped silica particles were identified to show high sensitivity to pH around the pKa of FITC (6.4), making them be potential useful as visualized pH indicator for detection of intracellular pH micro-circumstance.
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spelling pubmed-32206662011-11-21 FITC and Ru(phen)(3)(2+ )co-doped silica particles as visualized ratiometric pH indicator Xu, Jianquan Sun, Lei Li, Jun Liang, Jinglun Zhang, Huimao Yang, Wensheng Nanoscale Res Lett Nano Express The performance of fluorescein isothiocyanate (FITC) and tris(1, 10-phenanathroline) ruthenium ion (Ru(phen)(3)(2+)) co-doped silica particles as pH indicator was evaluated. The emission intensity ratios of the pH sensitive dye (FITC) and the reference dye (Ru(phen)(3)(2+)) in the particles were dependent on pH of the environment. The changes in emission intensity ratios of the two dyes under different pH could be measured under single excitation wavelength and readily visualized by naked eye under a 365-nm UV lamp. In particular, such FITC and Ru(phen)(3)(2+ )co-doped silica particles were identified to show high sensitivity to pH around the pKa of FITC (6.4), making them be potential useful as visualized pH indicator for detection of intracellular pH micro-circumstance. Springer 2011-10-25 /pmc/articles/PMC3220666/ /pubmed/22027093 http://dx.doi.org/10.1186/1556-276X-6-561 Text en Copyright ©2011 Xu et al; licensee Springer. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Nano Express
Xu, Jianquan
Sun, Lei
Li, Jun
Liang, Jinglun
Zhang, Huimao
Yang, Wensheng
FITC and Ru(phen)(3)(2+ )co-doped silica particles as visualized ratiometric pH indicator
title FITC and Ru(phen)(3)(2+ )co-doped silica particles as visualized ratiometric pH indicator
title_full FITC and Ru(phen)(3)(2+ )co-doped silica particles as visualized ratiometric pH indicator
title_fullStr FITC and Ru(phen)(3)(2+ )co-doped silica particles as visualized ratiometric pH indicator
title_full_unstemmed FITC and Ru(phen)(3)(2+ )co-doped silica particles as visualized ratiometric pH indicator
title_short FITC and Ru(phen)(3)(2+ )co-doped silica particles as visualized ratiometric pH indicator
title_sort fitc and ru(phen)(3)(2+ )co-doped silica particles as visualized ratiometric ph indicator
topic Nano Express
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3220666/
https://www.ncbi.nlm.nih.gov/pubmed/22027093
http://dx.doi.org/10.1186/1556-276X-6-561
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