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Strategies to facilitate the development of uncloned or cloned infectious full-length viral cDNAs: Apple chlorotic leaf spot virus as a case study

BACKGROUND: Approaches to simplify and streamline the construction of full-length infectious cDNA clones (FL-cDNAs) are needed. Among desirable improvements are the ability to use total nucleic acids (TNA) extracts from infected hosts (to bypass viral purification limitations) for the direct one-ste...

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Detalles Bibliográficos
Autores principales:	Youssef, Fater, Marais, Armelle, Faure, Chantal, Gentit, Pascal, Candresse, Thierry
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2011
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3220667/ https://www.ncbi.nlm.nih.gov/pubmed/22040379 http://dx.doi.org/10.1186/1743-422X-8-488

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