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Gut Microbiota of Healthy and Malnourished Children in Bangladesh
Poor health and malnutrition in preschool children are longstanding problems in Bangladesh. Gut microbiota plays a tremendous role in nutrient absorption and determining the state of health. In this study, metagenomic tool was employed to assess the gut microbiota composition of healthy and malnouri...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Research Foundation
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3221396/ https://www.ncbi.nlm.nih.gov/pubmed/22125551 http://dx.doi.org/10.3389/fmicb.2011.00228 |
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author | Monira, Shirajum Nakamura, Shota Gotoh, Kazuyoshi Izutsu, Kaori Watanabe, Haruo Alam, Nur Haque Endtz, Hubert Ph. Cravioto, Alejandro Ali, Sk. Imran Nakaya, Takaaki Horii, Toshihiro Iida, Tetsuya Alam, Munirul |
author_facet | Monira, Shirajum Nakamura, Shota Gotoh, Kazuyoshi Izutsu, Kaori Watanabe, Haruo Alam, Nur Haque Endtz, Hubert Ph. Cravioto, Alejandro Ali, Sk. Imran Nakaya, Takaaki Horii, Toshihiro Iida, Tetsuya Alam, Munirul |
author_sort | Monira, Shirajum |
collection | PubMed |
description | Poor health and malnutrition in preschool children are longstanding problems in Bangladesh. Gut microbiota plays a tremendous role in nutrient absorption and determining the state of health. In this study, metagenomic tool was employed to assess the gut microbiota composition of healthy and malnourished children. DNA was extracted from fecal samples of seven healthy and seven malnourished children (n = 14; age 2–3 years) were analyzed for the variable region of 16S rRNA genes by universal primer PCR followed by high-throughput 454 parallel sequencing to identify the bacterial phyla and genera. Our results reveal that the healthy children had a significantly higher number of operational taxonomic unit in their gut than that of the malnourished children (healthy vs. malnourished: 546 vs. 310). In malnourished children, bacterial population of the phyla Proteobacteria and Bacteroidetes accounted for 46 and 18%, respectively. Conversely, in healthy children, Proteobacteria and Bacteroidetes accounted for 5% and 44, respectively (p < 0.001). In malnourished children, the phylum Proteobacteria included pathogenic genera, namely Klebsiella and Escherichia, which were 174-fold and 9-fold higher, respectively, than their healthy counterpart. The predominance of potentially pathogenic Proteobacteria and minimal level of Bacteroidetes as commensal microbiota might be associated to the ill health of malnourished children in Bangladesh. |
format | Online Article Text |
id | pubmed-3221396 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Frontiers Research Foundation |
record_format | MEDLINE/PubMed |
spelling | pubmed-32213962011-11-28 Gut Microbiota of Healthy and Malnourished Children in Bangladesh Monira, Shirajum Nakamura, Shota Gotoh, Kazuyoshi Izutsu, Kaori Watanabe, Haruo Alam, Nur Haque Endtz, Hubert Ph. Cravioto, Alejandro Ali, Sk. Imran Nakaya, Takaaki Horii, Toshihiro Iida, Tetsuya Alam, Munirul Front Microbiol Microbiology Poor health and malnutrition in preschool children are longstanding problems in Bangladesh. Gut microbiota plays a tremendous role in nutrient absorption and determining the state of health. In this study, metagenomic tool was employed to assess the gut microbiota composition of healthy and malnourished children. DNA was extracted from fecal samples of seven healthy and seven malnourished children (n = 14; age 2–3 years) were analyzed for the variable region of 16S rRNA genes by universal primer PCR followed by high-throughput 454 parallel sequencing to identify the bacterial phyla and genera. Our results reveal that the healthy children had a significantly higher number of operational taxonomic unit in their gut than that of the malnourished children (healthy vs. malnourished: 546 vs. 310). In malnourished children, bacterial population of the phyla Proteobacteria and Bacteroidetes accounted for 46 and 18%, respectively. Conversely, in healthy children, Proteobacteria and Bacteroidetes accounted for 5% and 44, respectively (p < 0.001). In malnourished children, the phylum Proteobacteria included pathogenic genera, namely Klebsiella and Escherichia, which were 174-fold and 9-fold higher, respectively, than their healthy counterpart. The predominance of potentially pathogenic Proteobacteria and minimal level of Bacteroidetes as commensal microbiota might be associated to the ill health of malnourished children in Bangladesh. Frontiers Research Foundation 2011-11-21 /pmc/articles/PMC3221396/ /pubmed/22125551 http://dx.doi.org/10.3389/fmicb.2011.00228 Text en Copyright © 2011 Monira, Nakamura, Gotoh, Izutsu, Watanabe, Alam, Endtz, Cravioto, Ali, Nakaya, Horii, Iida and Alam. http://www.frontiersin.org/licenseagreement This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with. |
spellingShingle | Microbiology Monira, Shirajum Nakamura, Shota Gotoh, Kazuyoshi Izutsu, Kaori Watanabe, Haruo Alam, Nur Haque Endtz, Hubert Ph. Cravioto, Alejandro Ali, Sk. Imran Nakaya, Takaaki Horii, Toshihiro Iida, Tetsuya Alam, Munirul Gut Microbiota of Healthy and Malnourished Children in Bangladesh |
title | Gut Microbiota of Healthy and Malnourished Children in Bangladesh |
title_full | Gut Microbiota of Healthy and Malnourished Children in Bangladesh |
title_fullStr | Gut Microbiota of Healthy and Malnourished Children in Bangladesh |
title_full_unstemmed | Gut Microbiota of Healthy and Malnourished Children in Bangladesh |
title_short | Gut Microbiota of Healthy and Malnourished Children in Bangladesh |
title_sort | gut microbiota of healthy and malnourished children in bangladesh |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3221396/ https://www.ncbi.nlm.nih.gov/pubmed/22125551 http://dx.doi.org/10.3389/fmicb.2011.00228 |
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