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Gut Microbiota of Healthy and Malnourished Children in Bangladesh

Poor health and malnutrition in preschool children are longstanding problems in Bangladesh. Gut microbiota plays a tremendous role in nutrient absorption and determining the state of health. In this study, metagenomic tool was employed to assess the gut microbiota composition of healthy and malnouri...

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Autores principales: Monira, Shirajum, Nakamura, Shota, Gotoh, Kazuyoshi, Izutsu, Kaori, Watanabe, Haruo, Alam, Nur Haque, Endtz, Hubert Ph., Cravioto, Alejandro, Ali, Sk. Imran, Nakaya, Takaaki, Horii, Toshihiro, Iida, Tetsuya, Alam, Munirul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Research Foundation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3221396/
https://www.ncbi.nlm.nih.gov/pubmed/22125551
http://dx.doi.org/10.3389/fmicb.2011.00228
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author Monira, Shirajum
Nakamura, Shota
Gotoh, Kazuyoshi
Izutsu, Kaori
Watanabe, Haruo
Alam, Nur Haque
Endtz, Hubert Ph.
Cravioto, Alejandro
Ali, Sk. Imran
Nakaya, Takaaki
Horii, Toshihiro
Iida, Tetsuya
Alam, Munirul
author_facet Monira, Shirajum
Nakamura, Shota
Gotoh, Kazuyoshi
Izutsu, Kaori
Watanabe, Haruo
Alam, Nur Haque
Endtz, Hubert Ph.
Cravioto, Alejandro
Ali, Sk. Imran
Nakaya, Takaaki
Horii, Toshihiro
Iida, Tetsuya
Alam, Munirul
author_sort Monira, Shirajum
collection PubMed
description Poor health and malnutrition in preschool children are longstanding problems in Bangladesh. Gut microbiota plays a tremendous role in nutrient absorption and determining the state of health. In this study, metagenomic tool was employed to assess the gut microbiota composition of healthy and malnourished children. DNA was extracted from fecal samples of seven healthy and seven malnourished children (n = 14; age 2–3 years) were analyzed for the variable region of 16S rRNA genes by universal primer PCR followed by high-throughput 454 parallel sequencing to identify the bacterial phyla and genera. Our results reveal that the healthy children had a significantly higher number of operational taxonomic unit in their gut than that of the malnourished children (healthy vs. malnourished: 546 vs. 310). In malnourished children, bacterial population of the phyla Proteobacteria and Bacteroidetes accounted for 46 and 18%, respectively. Conversely, in healthy children, Proteobacteria and Bacteroidetes accounted for 5% and 44, respectively (p < 0.001). In malnourished children, the phylum Proteobacteria included pathogenic genera, namely Klebsiella and Escherichia, which were 174-fold and 9-fold higher, respectively, than their healthy counterpart. The predominance of potentially pathogenic Proteobacteria and minimal level of Bacteroidetes as commensal microbiota might be associated to the ill health of malnourished children in Bangladesh.
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spelling pubmed-32213962011-11-28 Gut Microbiota of Healthy and Malnourished Children in Bangladesh Monira, Shirajum Nakamura, Shota Gotoh, Kazuyoshi Izutsu, Kaori Watanabe, Haruo Alam, Nur Haque Endtz, Hubert Ph. Cravioto, Alejandro Ali, Sk. Imran Nakaya, Takaaki Horii, Toshihiro Iida, Tetsuya Alam, Munirul Front Microbiol Microbiology Poor health and malnutrition in preschool children are longstanding problems in Bangladesh. Gut microbiota plays a tremendous role in nutrient absorption and determining the state of health. In this study, metagenomic tool was employed to assess the gut microbiota composition of healthy and malnourished children. DNA was extracted from fecal samples of seven healthy and seven malnourished children (n = 14; age 2–3 years) were analyzed for the variable region of 16S rRNA genes by universal primer PCR followed by high-throughput 454 parallel sequencing to identify the bacterial phyla and genera. Our results reveal that the healthy children had a significantly higher number of operational taxonomic unit in their gut than that of the malnourished children (healthy vs. malnourished: 546 vs. 310). In malnourished children, bacterial population of the phyla Proteobacteria and Bacteroidetes accounted for 46 and 18%, respectively. Conversely, in healthy children, Proteobacteria and Bacteroidetes accounted for 5% and 44, respectively (p < 0.001). In malnourished children, the phylum Proteobacteria included pathogenic genera, namely Klebsiella and Escherichia, which were 174-fold and 9-fold higher, respectively, than their healthy counterpart. The predominance of potentially pathogenic Proteobacteria and minimal level of Bacteroidetes as commensal microbiota might be associated to the ill health of malnourished children in Bangladesh. Frontiers Research Foundation 2011-11-21 /pmc/articles/PMC3221396/ /pubmed/22125551 http://dx.doi.org/10.3389/fmicb.2011.00228 Text en Copyright © 2011 Monira, Nakamura, Gotoh, Izutsu, Watanabe, Alam, Endtz, Cravioto, Ali, Nakaya, Horii, Iida and Alam. http://www.frontiersin.org/licenseagreement This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with.
spellingShingle Microbiology
Monira, Shirajum
Nakamura, Shota
Gotoh, Kazuyoshi
Izutsu, Kaori
Watanabe, Haruo
Alam, Nur Haque
Endtz, Hubert Ph.
Cravioto, Alejandro
Ali, Sk. Imran
Nakaya, Takaaki
Horii, Toshihiro
Iida, Tetsuya
Alam, Munirul
Gut Microbiota of Healthy and Malnourished Children in Bangladesh
title Gut Microbiota of Healthy and Malnourished Children in Bangladesh
title_full Gut Microbiota of Healthy and Malnourished Children in Bangladesh
title_fullStr Gut Microbiota of Healthy and Malnourished Children in Bangladesh
title_full_unstemmed Gut Microbiota of Healthy and Malnourished Children in Bangladesh
title_short Gut Microbiota of Healthy and Malnourished Children in Bangladesh
title_sort gut microbiota of healthy and malnourished children in bangladesh
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3221396/
https://www.ncbi.nlm.nih.gov/pubmed/22125551
http://dx.doi.org/10.3389/fmicb.2011.00228
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