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The microRNA-Processing Enzyme Dicer Is Essential for Thyroid Function
Dicer is a type III ribonuclease required for the biogenesis of microRNAs (miRNAs), a class of small non-coding RNAs regulating gene expression at the post-transcriptional level. To explore the functional role of miRNAs in thyroid gland function, we generated a thyrocyte-specific Dicer conditional k...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3221669/ https://www.ncbi.nlm.nih.gov/pubmed/22132122 http://dx.doi.org/10.1371/journal.pone.0027648 |
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author | Frezzetti, Daniela Reale, Carla Calì, Gaetano Nitsch, Lucio Fagman, Henrik Nilsson, Ola Scarfò, Marzia De Vita, Gabriella Di Lauro, Roberto |
author_facet | Frezzetti, Daniela Reale, Carla Calì, Gaetano Nitsch, Lucio Fagman, Henrik Nilsson, Ola Scarfò, Marzia De Vita, Gabriella Di Lauro, Roberto |
author_sort | Frezzetti, Daniela |
collection | PubMed |
description | Dicer is a type III ribonuclease required for the biogenesis of microRNAs (miRNAs), a class of small non-coding RNAs regulating gene expression at the post-transcriptional level. To explore the functional role of miRNAs in thyroid gland function, we generated a thyrocyte-specific Dicer conditional knockout mouse. Here we show that development and early differentiation of the thyroid gland are not affected by the absence of Dicer, while severe hypothyroidism gradually develops after birth, leading to reduced body weight and shortened life span. Histological and molecular characterization of knockout mice reveals a dramatic loss of the thyroid gland follicular architecture associated with functional aberrations and down-regulation of several differentiation markers. The data presented in this study show for the first time that an intact miRNAs processing machinery is essential for thyroid physiology, suggesting that deregulation of specific miRNAs could be also involved in human thyroid dysfunctions. |
format | Online Article Text |
id | pubmed-3221669 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32216692011-11-30 The microRNA-Processing Enzyme Dicer Is Essential for Thyroid Function Frezzetti, Daniela Reale, Carla Calì, Gaetano Nitsch, Lucio Fagman, Henrik Nilsson, Ola Scarfò, Marzia De Vita, Gabriella Di Lauro, Roberto PLoS One Research Article Dicer is a type III ribonuclease required for the biogenesis of microRNAs (miRNAs), a class of small non-coding RNAs regulating gene expression at the post-transcriptional level. To explore the functional role of miRNAs in thyroid gland function, we generated a thyrocyte-specific Dicer conditional knockout mouse. Here we show that development and early differentiation of the thyroid gland are not affected by the absence of Dicer, while severe hypothyroidism gradually develops after birth, leading to reduced body weight and shortened life span. Histological and molecular characterization of knockout mice reveals a dramatic loss of the thyroid gland follicular architecture associated with functional aberrations and down-regulation of several differentiation markers. The data presented in this study show for the first time that an intact miRNAs processing machinery is essential for thyroid physiology, suggesting that deregulation of specific miRNAs could be also involved in human thyroid dysfunctions. Public Library of Science 2011-11-21 /pmc/articles/PMC3221669/ /pubmed/22132122 http://dx.doi.org/10.1371/journal.pone.0027648 Text en Frezzetti et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Frezzetti, Daniela Reale, Carla Calì, Gaetano Nitsch, Lucio Fagman, Henrik Nilsson, Ola Scarfò, Marzia De Vita, Gabriella Di Lauro, Roberto The microRNA-Processing Enzyme Dicer Is Essential for Thyroid Function |
title | The microRNA-Processing Enzyme Dicer Is Essential for Thyroid Function |
title_full | The microRNA-Processing Enzyme Dicer Is Essential for Thyroid Function |
title_fullStr | The microRNA-Processing Enzyme Dicer Is Essential for Thyroid Function |
title_full_unstemmed | The microRNA-Processing Enzyme Dicer Is Essential for Thyroid Function |
title_short | The microRNA-Processing Enzyme Dicer Is Essential for Thyroid Function |
title_sort | microrna-processing enzyme dicer is essential for thyroid function |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3221669/ https://www.ncbi.nlm.nih.gov/pubmed/22132122 http://dx.doi.org/10.1371/journal.pone.0027648 |
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