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The Nature of the Dietary Protein Impacts the Tissue-to-Diet (15)N Discrimination Factors in Laboratory Rats

Due to the existence of isotope effects on some metabolic pathways of amino acid and protein metabolism, animal tissues are (15)N-enriched relative to their dietary nitrogen sources and this (15)N enrichment varies among different tissues and metabolic pools. The magnitude of the tissue-to-diet disc...

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Autores principales: Poupin, Nathalie, Bos, Cécile, Mariotti, François, Huneau, Jean-François, Tomé, Daniel, Fouillet, Hélène
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3222673/
https://www.ncbi.nlm.nih.gov/pubmed/22132207
http://dx.doi.org/10.1371/journal.pone.0028046
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author Poupin, Nathalie
Bos, Cécile
Mariotti, François
Huneau, Jean-François
Tomé, Daniel
Fouillet, Hélène
author_facet Poupin, Nathalie
Bos, Cécile
Mariotti, François
Huneau, Jean-François
Tomé, Daniel
Fouillet, Hélène
author_sort Poupin, Nathalie
collection PubMed
description Due to the existence of isotope effects on some metabolic pathways of amino acid and protein metabolism, animal tissues are (15)N-enriched relative to their dietary nitrogen sources and this (15)N enrichment varies among different tissues and metabolic pools. The magnitude of the tissue-to-diet discrimination (Δ(15)N) has also been shown to depend on dietary factors. Since dietary protein sources affect amino acid and protein metabolism, we hypothesized that they would impact this discrimination factor, with selective effects at the tissue level. To test this hypothesis, we investigated in rats the influence of a milk or soy protein-based diet on Δ(15)N in various nitrogen fractions (urea, protein and non-protein fractions) of blood and tissues, focusing on visceral tissues. Regardless of the diet, the different protein fractions of blood and tissues were generally (15)N-enriched relative to their non-protein fraction and to the diet (Δ(15)N>0), with large variations in the Δ(15)N between tissue proteins. Δ(15)N values were markedly lower in tissue proteins of rats fed milk proteins compared to those fed soy proteins, in all sampled tissues except in the intestine, and the amplitude of Δ(15)N differences between diets differed between tissues. Both between-tissue and between-diet Δ(15)N differences are probably related to modulations of the relative orientation of dietary and endogenous amino acids in the different metabolic pathways. More specifically, the smaller Δ(15)N values observed in tissue proteins with milk than soy dietary protein may be due to a slightly more direct channeling of dietary amino acids for tissue protein renewal and to a lower recycling of amino acids through fractionating pathways. In conclusion, the present data indicate that natural Δ(15)N of tissue are sensitive markers of the specific subtle regional modifications of the protein and amino acid metabolism induced by the protein dietary source.
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spelling pubmed-32226732011-11-30 The Nature of the Dietary Protein Impacts the Tissue-to-Diet (15)N Discrimination Factors in Laboratory Rats Poupin, Nathalie Bos, Cécile Mariotti, François Huneau, Jean-François Tomé, Daniel Fouillet, Hélène PLoS One Research Article Due to the existence of isotope effects on some metabolic pathways of amino acid and protein metabolism, animal tissues are (15)N-enriched relative to their dietary nitrogen sources and this (15)N enrichment varies among different tissues and metabolic pools. The magnitude of the tissue-to-diet discrimination (Δ(15)N) has also been shown to depend on dietary factors. Since dietary protein sources affect amino acid and protein metabolism, we hypothesized that they would impact this discrimination factor, with selective effects at the tissue level. To test this hypothesis, we investigated in rats the influence of a milk or soy protein-based diet on Δ(15)N in various nitrogen fractions (urea, protein and non-protein fractions) of blood and tissues, focusing on visceral tissues. Regardless of the diet, the different protein fractions of blood and tissues were generally (15)N-enriched relative to their non-protein fraction and to the diet (Δ(15)N>0), with large variations in the Δ(15)N between tissue proteins. Δ(15)N values were markedly lower in tissue proteins of rats fed milk proteins compared to those fed soy proteins, in all sampled tissues except in the intestine, and the amplitude of Δ(15)N differences between diets differed between tissues. Both between-tissue and between-diet Δ(15)N differences are probably related to modulations of the relative orientation of dietary and endogenous amino acids in the different metabolic pathways. More specifically, the smaller Δ(15)N values observed in tissue proteins with milk than soy dietary protein may be due to a slightly more direct channeling of dietary amino acids for tissue protein renewal and to a lower recycling of amino acids through fractionating pathways. In conclusion, the present data indicate that natural Δ(15)N of tissue are sensitive markers of the specific subtle regional modifications of the protein and amino acid metabolism induced by the protein dietary source. Public Library of Science 2011-11-22 /pmc/articles/PMC3222673/ /pubmed/22132207 http://dx.doi.org/10.1371/journal.pone.0028046 Text en Poupin et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Poupin, Nathalie
Bos, Cécile
Mariotti, François
Huneau, Jean-François
Tomé, Daniel
Fouillet, Hélène
The Nature of the Dietary Protein Impacts the Tissue-to-Diet (15)N Discrimination Factors in Laboratory Rats
title The Nature of the Dietary Protein Impacts the Tissue-to-Diet (15)N Discrimination Factors in Laboratory Rats
title_full The Nature of the Dietary Protein Impacts the Tissue-to-Diet (15)N Discrimination Factors in Laboratory Rats
title_fullStr The Nature of the Dietary Protein Impacts the Tissue-to-Diet (15)N Discrimination Factors in Laboratory Rats
title_full_unstemmed The Nature of the Dietary Protein Impacts the Tissue-to-Diet (15)N Discrimination Factors in Laboratory Rats
title_short The Nature of the Dietary Protein Impacts the Tissue-to-Diet (15)N Discrimination Factors in Laboratory Rats
title_sort nature of the dietary protein impacts the tissue-to-diet (15)n discrimination factors in laboratory rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3222673/
https://www.ncbi.nlm.nih.gov/pubmed/22132207
http://dx.doi.org/10.1371/journal.pone.0028046
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