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Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems

A crucial bottleneck in membrane protein studies, particularly G-protein coupled receptors, is the notorious difficulty of finding an optimal detergent that can solubilize them and maintain their stability and function. Here we report rapid production of 12 unique mammalian olfactory receptors using...

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Autores principales: Corin, Karolina, Baaske, Philipp, Ravel, Deepali B., Song, Junyao, Brown, Emily, Wang, Xiaoqiang, Wienken, Christoph J., Jerabek-Willemsen, Moran, Duhr, Stefan, Luo, Yuan, Braun, Dieter, Zhang, Shuguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3223156/
https://www.ncbi.nlm.nih.gov/pubmed/22132066
http://dx.doi.org/10.1371/journal.pone.0025067
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author Corin, Karolina
Baaske, Philipp
Ravel, Deepali B.
Song, Junyao
Brown, Emily
Wang, Xiaoqiang
Wienken, Christoph J.
Jerabek-Willemsen, Moran
Duhr, Stefan
Luo, Yuan
Braun, Dieter
Zhang, Shuguang
author_facet Corin, Karolina
Baaske, Philipp
Ravel, Deepali B.
Song, Junyao
Brown, Emily
Wang, Xiaoqiang
Wienken, Christoph J.
Jerabek-Willemsen, Moran
Duhr, Stefan
Luo, Yuan
Braun, Dieter
Zhang, Shuguang
author_sort Corin, Karolina
collection PubMed
description A crucial bottleneck in membrane protein studies, particularly G-protein coupled receptors, is the notorious difficulty of finding an optimal detergent that can solubilize them and maintain their stability and function. Here we report rapid production of 12 unique mammalian olfactory receptors using short designer lipid-like peptides as detergents. The peptides were able to solubilize and stabilize each receptor. Circular dichroism showed that the purified olfactory receptors had alpha-helical secondary structures. Microscale thermophoresis suggested that the receptors were functional and bound their odorants. Blot intensity measurements indicated that milligram quantities of each olfactory receptor could be produced with at least one peptide detergent. The peptide detergents' capability was comparable to that of the detergent Brij-35. The ability of 10 peptide detergents to functionally solubilize 12 olfactory receptors demonstrates their usefulness as a new class of detergents for olfactory receptors, and possibly other G-protein coupled receptors and membrane proteins.
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spelling pubmed-32231562011-11-30 Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems Corin, Karolina Baaske, Philipp Ravel, Deepali B. Song, Junyao Brown, Emily Wang, Xiaoqiang Wienken, Christoph J. Jerabek-Willemsen, Moran Duhr, Stefan Luo, Yuan Braun, Dieter Zhang, Shuguang PLoS One Research Article A crucial bottleneck in membrane protein studies, particularly G-protein coupled receptors, is the notorious difficulty of finding an optimal detergent that can solubilize them and maintain their stability and function. Here we report rapid production of 12 unique mammalian olfactory receptors using short designer lipid-like peptides as detergents. The peptides were able to solubilize and stabilize each receptor. Circular dichroism showed that the purified olfactory receptors had alpha-helical secondary structures. Microscale thermophoresis suggested that the receptors were functional and bound their odorants. Blot intensity measurements indicated that milligram quantities of each olfactory receptor could be produced with at least one peptide detergent. The peptide detergents' capability was comparable to that of the detergent Brij-35. The ability of 10 peptide detergents to functionally solubilize 12 olfactory receptors demonstrates their usefulness as a new class of detergents for olfactory receptors, and possibly other G-protein coupled receptors and membrane proteins. Public Library of Science 2011-11-23 /pmc/articles/PMC3223156/ /pubmed/22132066 http://dx.doi.org/10.1371/journal.pone.0025067 Text en Corin et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Corin, Karolina
Baaske, Philipp
Ravel, Deepali B.
Song, Junyao
Brown, Emily
Wang, Xiaoqiang
Wienken, Christoph J.
Jerabek-Willemsen, Moran
Duhr, Stefan
Luo, Yuan
Braun, Dieter
Zhang, Shuguang
Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems
title Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems
title_full Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems
title_fullStr Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems
title_full_unstemmed Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems
title_short Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems
title_sort designer lipid-like peptides: a class of detergents for studying functional olfactory receptors using commercial cell-free systems
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3223156/
https://www.ncbi.nlm.nih.gov/pubmed/22132066
http://dx.doi.org/10.1371/journal.pone.0025067
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