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Bacteria Associated with Copestylum (Diptera, Syrphidae) Larvae and Their Cactus Host Isolatocereus dumortieri
We describe the gut bacterial diversity inhabiting two saprophagous syrphids and their breeding substrate (decayed tissues of the columnar cactus Isolatocereus dumortieri). We analyzed the gut microbiota of Copestylum latum (scooping larvae that feed on decayed cactus tissues) and Copestylum limbipe...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3223168/ https://www.ncbi.nlm.nih.gov/pubmed/22132101 http://dx.doi.org/10.1371/journal.pone.0027443 |
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author | Martínez-Falcón, Ana Paola Durbán, Ana Latorre, Amparo Antón, Josefa Marcos-García, María de los Ángeles |
author_facet | Martínez-Falcón, Ana Paola Durbán, Ana Latorre, Amparo Antón, Josefa Marcos-García, María de los Ángeles |
author_sort | Martínez-Falcón, Ana Paola |
collection | PubMed |
description | We describe the gut bacterial diversity inhabiting two saprophagous syrphids and their breeding substrate (decayed tissues of the columnar cactus Isolatocereus dumortieri). We analyzed the gut microbiota of Copestylum latum (scooping larvae that feed on decayed cactus tissues) and Copestylum limbipenne (whose larvae can also feed on semiliquid tissues) using molecular techniques. DNA was extracted from larval guts and cactus tissues. The V1-V3 region of the 16S rRNA genes was amplified and sequenced. A total of 31079 sequences were obtained. The main findings are: C. limbipenne is dominated by several Enterobacteriaceae, including putative nitrogen-fixing genera and pectinolitic species and some denitrifying species, whereas in C. latum unclassified Gammaproteobacteria predominate. Decayed tissues have a dominant lactic acid bacterial community. The bacterial communities were more similar between larval species than between each larva and its breeding substrate. The results suggest that the gut bacterial community in these insects is not strongly affected by diet and must be dependent on other factors, such as vertical transmission, evolutionary history and host innate immunity. |
format | Online Article Text |
id | pubmed-3223168 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32231682011-11-30 Bacteria Associated with Copestylum (Diptera, Syrphidae) Larvae and Their Cactus Host Isolatocereus dumortieri Martínez-Falcón, Ana Paola Durbán, Ana Latorre, Amparo Antón, Josefa Marcos-García, María de los Ángeles PLoS One Research Article We describe the gut bacterial diversity inhabiting two saprophagous syrphids and their breeding substrate (decayed tissues of the columnar cactus Isolatocereus dumortieri). We analyzed the gut microbiota of Copestylum latum (scooping larvae that feed on decayed cactus tissues) and Copestylum limbipenne (whose larvae can also feed on semiliquid tissues) using molecular techniques. DNA was extracted from larval guts and cactus tissues. The V1-V3 region of the 16S rRNA genes was amplified and sequenced. A total of 31079 sequences were obtained. The main findings are: C. limbipenne is dominated by several Enterobacteriaceae, including putative nitrogen-fixing genera and pectinolitic species and some denitrifying species, whereas in C. latum unclassified Gammaproteobacteria predominate. Decayed tissues have a dominant lactic acid bacterial community. The bacterial communities were more similar between larval species than between each larva and its breeding substrate. The results suggest that the gut bacterial community in these insects is not strongly affected by diet and must be dependent on other factors, such as vertical transmission, evolutionary history and host innate immunity. Public Library of Science 2011-11-23 /pmc/articles/PMC3223168/ /pubmed/22132101 http://dx.doi.org/10.1371/journal.pone.0027443 Text en Martínez-Falcón et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Martínez-Falcón, Ana Paola Durbán, Ana Latorre, Amparo Antón, Josefa Marcos-García, María de los Ángeles Bacteria Associated with Copestylum (Diptera, Syrphidae) Larvae and Their Cactus Host Isolatocereus dumortieri |
title | Bacteria Associated with Copestylum (Diptera, Syrphidae) Larvae and Their Cactus Host Isolatocereus dumortieri
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title_full | Bacteria Associated with Copestylum (Diptera, Syrphidae) Larvae and Their Cactus Host Isolatocereus dumortieri
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title_fullStr | Bacteria Associated with Copestylum (Diptera, Syrphidae) Larvae and Their Cactus Host Isolatocereus dumortieri
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title_full_unstemmed | Bacteria Associated with Copestylum (Diptera, Syrphidae) Larvae and Their Cactus Host Isolatocereus dumortieri
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title_short | Bacteria Associated with Copestylum (Diptera, Syrphidae) Larvae and Their Cactus Host Isolatocereus dumortieri
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title_sort | bacteria associated with copestylum (diptera, syrphidae) larvae and their cactus host isolatocereus dumortieri |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3223168/ https://www.ncbi.nlm.nih.gov/pubmed/22132101 http://dx.doi.org/10.1371/journal.pone.0027443 |
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