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NORMA-Gene: A simple and robust method for qPCR normalization based on target gene data

BACKGROUND: Normalization of target gene expression, measured by real-time quantitative PCR (qPCR), is a requirement for reducing experimental bias and thereby improving data quality. The currently used normalization approach is based on using one or more reference genes. Yet, this approach extends...

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Detalles Bibliográficos
Autores principales: Heckmann, Lars-Henrik, Sørensen, Peter B, Krogh, Paul Henning, Sørensen, Jesper G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3223928/
https://www.ncbi.nlm.nih.gov/pubmed/21693017
http://dx.doi.org/10.1186/1471-2105-12-250