RNase H-dependent PCR (rhPCR): improved specificity and single nucleotide polymorphism detection using blocked cleavable primers

BACKGROUND: The polymerase chain reaction (PCR) is commonly used to detect the presence of nucleic acid sequences both in research and diagnostic settings. While high specificity is often achieved, biological requirements sometimes necessitate that primers are placed in suboptimal locations which le...

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Detalles Bibliográficos
Autores principales: Dobosy, Joseph R, Rose, Scott D, Beltz, Kristin R, Rupp, Susan M, Powers, Kristy M, Behlke, Mark A, Walder, Joseph A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3224242/
https://www.ncbi.nlm.nih.gov/pubmed/21831278
http://dx.doi.org/10.1186/1472-6750-11-80

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