Leaf extracts from Nitraria retusa promote cell population growth of human cancer cells by inducing apoptosis

BACKGROUND: In this report the phytochemical profile of Nitraria. Retusa (N. Retusa) leaf extracts were identified and their ability to induce apoptosis in human chronic myelogenous erythroleukaemia (K562) was evaluated. METHODS: Apoptosis of the human chronic myelogenous erythroleukaemia (K562) was...

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Autores principales: Boubaker, Jihed, Bhouri, Wissem, Sghaier, Mohamed Ben, Bouhlel, Ines, Skandrani, Ines, Ghedira, Kamel, Chekir-Ghedira, Leila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Primary Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3224376/
https://www.ncbi.nlm.nih.gov/pubmed/22040460
http://dx.doi.org/10.1186/1475-2867-11-37
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author Boubaker, Jihed
Bhouri, Wissem
Sghaier, Mohamed Ben
Bouhlel, Ines
Skandrani, Ines
Ghedira, Kamel
Chekir-Ghedira, Leila
author_facet Boubaker, Jihed
Bhouri, Wissem
Sghaier, Mohamed Ben
Bouhlel, Ines
Skandrani, Ines
Ghedira, Kamel
Chekir-Ghedira, Leila
author_sort Boubaker, Jihed
collection PubMed
description BACKGROUND: In this report the phytochemical profile of Nitraria. Retusa (N. Retusa) leaf extracts were identified and their ability to induce apoptosis in human chronic myelogenous erythroleukaemia (K562) was evaluated. METHODS: Apoptosis of the human chronic myelogenous erythroleukaemia (K562) was evidenced by investigating DNA fragmentation, PARP cleavage and caspases 3 and 8 inducing activities, in the presence of N. retusa extracts. RESULTS: Our study revealed that the tested extracts from N. Retusa contain many useful bioactive compounds. They induced in a time-dependent manner the apoptosis the tested cancerous our cell line. This result was confirmed by ladder DNA fragmentation profile and PARP cleavage, as well as a release in caspase-3 and caspase-8 level. CONCLUSION: Our results indicate that the tested compounds have a significant antiproliferative effect which may be due to their involvement in the induction of the extrinsic apoptosic pathway.
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spelling pubmed-32243762011-11-30 Leaf extracts from Nitraria retusa promote cell population growth of human cancer cells by inducing apoptosis Boubaker, Jihed Bhouri, Wissem Sghaier, Mohamed Ben Bouhlel, Ines Skandrani, Ines Ghedira, Kamel Chekir-Ghedira, Leila Cancer Cell Int Primary Research BACKGROUND: In this report the phytochemical profile of Nitraria. Retusa (N. Retusa) leaf extracts were identified and their ability to induce apoptosis in human chronic myelogenous erythroleukaemia (K562) was evaluated. METHODS: Apoptosis of the human chronic myelogenous erythroleukaemia (K562) was evidenced by investigating DNA fragmentation, PARP cleavage and caspases 3 and 8 inducing activities, in the presence of N. retusa extracts. RESULTS: Our study revealed that the tested extracts from N. Retusa contain many useful bioactive compounds. They induced in a time-dependent manner the apoptosis the tested cancerous our cell line. This result was confirmed by ladder DNA fragmentation profile and PARP cleavage, as well as a release in caspase-3 and caspase-8 level. CONCLUSION: Our results indicate that the tested compounds have a significant antiproliferative effect which may be due to their involvement in the induction of the extrinsic apoptosic pathway. BioMed Central 2011-10-31 /pmc/articles/PMC3224376/ /pubmed/22040460 http://dx.doi.org/10.1186/1475-2867-11-37 Text en Copyright ©2011 Boubaker et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Primary Research
Boubaker, Jihed
Bhouri, Wissem
Sghaier, Mohamed Ben
Bouhlel, Ines
Skandrani, Ines
Ghedira, Kamel
Chekir-Ghedira, Leila
Leaf extracts from Nitraria retusa promote cell population growth of human cancer cells by inducing apoptosis
title Leaf extracts from Nitraria retusa promote cell population growth of human cancer cells by inducing apoptosis
title_full Leaf extracts from Nitraria retusa promote cell population growth of human cancer cells by inducing apoptosis
title_fullStr Leaf extracts from Nitraria retusa promote cell population growth of human cancer cells by inducing apoptosis
title_full_unstemmed Leaf extracts from Nitraria retusa promote cell population growth of human cancer cells by inducing apoptosis
title_short Leaf extracts from Nitraria retusa promote cell population growth of human cancer cells by inducing apoptosis
title_sort leaf extracts from nitraria retusa promote cell population growth of human cancer cells by inducing apoptosis
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3224376/
https://www.ncbi.nlm.nih.gov/pubmed/22040460
http://dx.doi.org/10.1186/1475-2867-11-37
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