Formulation and Evaluation of Long Circulating Liposomal Amphotericin B: A Scinti-kinetic Study using (99m)Tc in BALB/C Mice

In the present study, we formulated long circulating liposomes for amphotericin B and characterized them. The formulation was optimized using 2(3) factorial designs. Pegylated liposomal formulation showed favorable results with reference to particle size (247.33±9.60 nm), percent entrapment efficien...

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Autores principales: Jadhav, M. P., Nagarsenker, Mangal S., Gaikwad, R. V., Samad, A., Kshirsagar, Nilima A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2011
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3224410/
https://www.ncbi.nlm.nih.gov/pubmed/22131622
http://dx.doi.org/10.4103/0250-474X.89757
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author Jadhav, M. P.
Nagarsenker, Mangal S.
Gaikwad, R. V.
Samad, A.
Kshirsagar, Nilima A.
author_facet Jadhav, M. P.
Nagarsenker, Mangal S.
Gaikwad, R. V.
Samad, A.
Kshirsagar, Nilima A.
author_sort Jadhav, M. P.
collection PubMed
description In the present study, we formulated long circulating liposomes for amphotericin B and characterized them. The formulation was optimized using 2(3) factorial designs. Pegylated liposomal formulation showed favorable results with reference to particle size (247.33±9.60 nm), percent entrapment efficiency (94.55±3.34%). TEM studies revealed that the liposomes were essentially spherical, hollow, and appeared like powder puff structures. From DSC study it was concluded that the pegylated formulation containing Amp B showed better stability and membrane integrity of the formulation. During the stability studies the formulation was found to be stable. When subjected to gamma scintigraphy kinetic tracer studies the formulation showed longer residence time in the blood in BALB/C mice.
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spelling pubmed-32244102011-11-30 Formulation and Evaluation of Long Circulating Liposomal Amphotericin B: A Scinti-kinetic Study using (99m)Tc in BALB/C Mice Jadhav, M. P. Nagarsenker, Mangal S. Gaikwad, R. V. Samad, A. Kshirsagar, Nilima A. Indian J Pharm Sci Research Paper In the present study, we formulated long circulating liposomes for amphotericin B and characterized them. The formulation was optimized using 2(3) factorial designs. Pegylated liposomal formulation showed favorable results with reference to particle size (247.33±9.60 nm), percent entrapment efficiency (94.55±3.34%). TEM studies revealed that the liposomes were essentially spherical, hollow, and appeared like powder puff structures. From DSC study it was concluded that the pegylated formulation containing Amp B showed better stability and membrane integrity of the formulation. During the stability studies the formulation was found to be stable. When subjected to gamma scintigraphy kinetic tracer studies the formulation showed longer residence time in the blood in BALB/C mice. Medknow Publications & Media Pvt Ltd 2011 /pmc/articles/PMC3224410/ /pubmed/22131622 http://dx.doi.org/10.4103/0250-474X.89757 Text en Copyright: © Indian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Jadhav, M. P.
Nagarsenker, Mangal S.
Gaikwad, R. V.
Samad, A.
Kshirsagar, Nilima A.
Formulation and Evaluation of Long Circulating Liposomal Amphotericin B: A Scinti-kinetic Study using (99m)Tc in BALB/C Mice
title Formulation and Evaluation of Long Circulating Liposomal Amphotericin B: A Scinti-kinetic Study using (99m)Tc in BALB/C Mice
title_full Formulation and Evaluation of Long Circulating Liposomal Amphotericin B: A Scinti-kinetic Study using (99m)Tc in BALB/C Mice
title_fullStr Formulation and Evaluation of Long Circulating Liposomal Amphotericin B: A Scinti-kinetic Study using (99m)Tc in BALB/C Mice
title_full_unstemmed Formulation and Evaluation of Long Circulating Liposomal Amphotericin B: A Scinti-kinetic Study using (99m)Tc in BALB/C Mice
title_short Formulation and Evaluation of Long Circulating Liposomal Amphotericin B: A Scinti-kinetic Study using (99m)Tc in BALB/C Mice
title_sort formulation and evaluation of long circulating liposomal amphotericin b: a scinti-kinetic study using (99m)tc in balb/c mice
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3224410/
https://www.ncbi.nlm.nih.gov/pubmed/22131622
http://dx.doi.org/10.4103/0250-474X.89757
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