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Development and evaluation of pH-responsive single-walled carbon nanotube-doxorubicin complexes in cancer cells
Single-walled carbon nanotubes (SWNTs) have been identified as an efficient drug carrier. Here a controlled drug-delivery system based on SWNTs coated with doxorubicin (DOX) through hydrazone bonds was developed, because the hydrazone bond is more sensitive to tumor microenvironments than other cova...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3224716/ https://www.ncbi.nlm.nih.gov/pubmed/22131835 http://dx.doi.org/10.2147/IJN.S25162 |
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author | Gu, Yan-Juan Cheng, Jinping Jin, Jiefu Cheng, Shuk Han Wong, Wing-Tak |
author_facet | Gu, Yan-Juan Cheng, Jinping Jin, Jiefu Cheng, Shuk Han Wong, Wing-Tak |
author_sort | Gu, Yan-Juan |
collection | PubMed |
description | Single-walled carbon nanotubes (SWNTs) have been identified as an efficient drug carrier. Here a controlled drug-delivery system based on SWNTs coated with doxorubicin (DOX) through hydrazone bonds was developed, because the hydrazone bond is more sensitive to tumor microenvironments than other covalent linkers. The SWNTs were firstly stabilized with polyethylene glycol (H(2)N-PEG-NH(2)). Hydrazinobenzoic acid (HBA) was then covalently attached on SWNTs via carbodiimide-activated coupling reaction to form hydrazine-modified SWNTs. The anticancer drug DOX was conjugated to the HBA segments of SWNT using hydrazine as the linker. The resulting hydrazone bonds formed between the DOX molecules and the HBA segments of SWNTs are acid cleavable, thereby providing a strong pH-responsive drug release, which may facilitate effective DOX release near the acidic tumor microenvironment and thus reduce its overall systemic toxicity. The DOX-loaded SWNTs were efficiently taken up by HepG2 tumor cells, and DOX was released intracellularly, as revealed by MTT assay and confocal microscope observations. Compared with SWNT-DOX conjugate formed by supramolecular interaction, the SWNT-HBA-DOX featured high weight loading and prolonged release of DOX, and thus improved its cytotoxicity against cancer cells. This study suggests that while SWNTs have great potential as a drug carrier, the efficient formulation strategy requires further study. |
format | Online Article Text |
id | pubmed-3224716 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-32247162011-11-30 Development and evaluation of pH-responsive single-walled carbon nanotube-doxorubicin complexes in cancer cells Gu, Yan-Juan Cheng, Jinping Jin, Jiefu Cheng, Shuk Han Wong, Wing-Tak Int J Nanomedicine Original Research Single-walled carbon nanotubes (SWNTs) have been identified as an efficient drug carrier. Here a controlled drug-delivery system based on SWNTs coated with doxorubicin (DOX) through hydrazone bonds was developed, because the hydrazone bond is more sensitive to tumor microenvironments than other covalent linkers. The SWNTs were firstly stabilized with polyethylene glycol (H(2)N-PEG-NH(2)). Hydrazinobenzoic acid (HBA) was then covalently attached on SWNTs via carbodiimide-activated coupling reaction to form hydrazine-modified SWNTs. The anticancer drug DOX was conjugated to the HBA segments of SWNT using hydrazine as the linker. The resulting hydrazone bonds formed between the DOX molecules and the HBA segments of SWNTs are acid cleavable, thereby providing a strong pH-responsive drug release, which may facilitate effective DOX release near the acidic tumor microenvironment and thus reduce its overall systemic toxicity. The DOX-loaded SWNTs were efficiently taken up by HepG2 tumor cells, and DOX was released intracellularly, as revealed by MTT assay and confocal microscope observations. Compared with SWNT-DOX conjugate formed by supramolecular interaction, the SWNT-HBA-DOX featured high weight loading and prolonged release of DOX, and thus improved its cytotoxicity against cancer cells. This study suggests that while SWNTs have great potential as a drug carrier, the efficient formulation strategy requires further study. Dove Medical Press 2011 2011-11-17 /pmc/articles/PMC3224716/ /pubmed/22131835 http://dx.doi.org/10.2147/IJN.S25162 Text en © 2011 Gu et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Gu, Yan-Juan Cheng, Jinping Jin, Jiefu Cheng, Shuk Han Wong, Wing-Tak Development and evaluation of pH-responsive single-walled carbon nanotube-doxorubicin complexes in cancer cells |
title | Development and evaluation of pH-responsive single-walled carbon nanotube-doxorubicin complexes in cancer cells |
title_full | Development and evaluation of pH-responsive single-walled carbon nanotube-doxorubicin complexes in cancer cells |
title_fullStr | Development and evaluation of pH-responsive single-walled carbon nanotube-doxorubicin complexes in cancer cells |
title_full_unstemmed | Development and evaluation of pH-responsive single-walled carbon nanotube-doxorubicin complexes in cancer cells |
title_short | Development and evaluation of pH-responsive single-walled carbon nanotube-doxorubicin complexes in cancer cells |
title_sort | development and evaluation of ph-responsive single-walled carbon nanotube-doxorubicin complexes in cancer cells |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3224716/ https://www.ncbi.nlm.nih.gov/pubmed/22131835 http://dx.doi.org/10.2147/IJN.S25162 |
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