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Inhibition of experimental myopia by a dopamine agonist: different effectiveness between form deprivation and hyperopic defocus in guinea pigs

PURPOSE: The dopamine (DA) system in the retina is critical to normal visual development as lack of retinal DA signaling may contribute to myopic development. The involvement of DA in myopic development is complex and may be different between form deprivation and hyperopic defocus. This study evalua...

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Autores principales: Dong, Feng, Zhi, Zhina, Pan, Miaozhen, Xie, Ruozhong, Qin, Xiaoyi, Lu, Runxia, Mao, Xinjie, Chen, Jiang-Fan, Willcox, Mark D.P., Qu, Jia, Zhou, Xiangtian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Vision 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3224832/
https://www.ncbi.nlm.nih.gov/pubmed/22128230
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author Dong, Feng
Zhi, Zhina
Pan, Miaozhen
Xie, Ruozhong
Qin, Xiaoyi
Lu, Runxia
Mao, Xinjie
Chen, Jiang-Fan
Willcox, Mark D.P.
Qu, Jia
Zhou, Xiangtian
author_facet Dong, Feng
Zhi, Zhina
Pan, Miaozhen
Xie, Ruozhong
Qin, Xiaoyi
Lu, Runxia
Mao, Xinjie
Chen, Jiang-Fan
Willcox, Mark D.P.
Qu, Jia
Zhou, Xiangtian
author_sort Dong, Feng
collection PubMed
description PURPOSE: The dopamine (DA) system in the retina is critical to normal visual development as lack of retinal DA signaling may contribute to myopic development. The involvement of DA in myopic development is complex and may be different between form deprivation and hyperopic defocus. This study evaluated effects of a non-selective DA receptor agonist, apomorphine (APO) on refractive development in guinea pigs treated with form deprivation or hyperopic defocus. METHODS: APO was subconjunctivally injected daily for 11 days in form-deprived (0.025 to 2.5 ng/µl) and defocused (0.025 to 250 ng/µl) eyes. Changes in ocular biometry and retinal concentration of DA and its metabolites (DOPAC) were measured in the 2 animal models to assess the level of DA involvement in each of the models (the less the change, the lower the involvement). RESULTS: Similar myopic degree was induced in both the deprived and defocused eyes (−4.06 D versus −3.64 D) at 11 days of the experiment. DA and DOPAC levels were reduced in the deprived eyes but did not change significantly in the defocused eyes compared to the fellow and normal control eyes. A subconjunctival injection of APO daily for 11 days at concentrations ranged from 0.025 to 2.5 ng/µl inhibited form deprivation myopia in a concentration-dependent manner. By contrast, the APO treatment ranged from 0.025 to 250 ng/µl did not effectively inhibit the defocus-induced myopia and the associated axial elongation. CONCLUSIONS: DA signaling may play a more critical role in form deprivation myopia than in defocus-induced myopia, raising a question whether the mechanisms of DA signaling are different under these two types of experimental myopia.
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spelling pubmed-32248322011-11-29 Inhibition of experimental myopia by a dopamine agonist: different effectiveness between form deprivation and hyperopic defocus in guinea pigs Dong, Feng Zhi, Zhina Pan, Miaozhen Xie, Ruozhong Qin, Xiaoyi Lu, Runxia Mao, Xinjie Chen, Jiang-Fan Willcox, Mark D.P. Qu, Jia Zhou, Xiangtian Mol Vis Research Article PURPOSE: The dopamine (DA) system in the retina is critical to normal visual development as lack of retinal DA signaling may contribute to myopic development. The involvement of DA in myopic development is complex and may be different between form deprivation and hyperopic defocus. This study evaluated effects of a non-selective DA receptor agonist, apomorphine (APO) on refractive development in guinea pigs treated with form deprivation or hyperopic defocus. METHODS: APO was subconjunctivally injected daily for 11 days in form-deprived (0.025 to 2.5 ng/µl) and defocused (0.025 to 250 ng/µl) eyes. Changes in ocular biometry and retinal concentration of DA and its metabolites (DOPAC) were measured in the 2 animal models to assess the level of DA involvement in each of the models (the less the change, the lower the involvement). RESULTS: Similar myopic degree was induced in both the deprived and defocused eyes (−4.06 D versus −3.64 D) at 11 days of the experiment. DA and DOPAC levels were reduced in the deprived eyes but did not change significantly in the defocused eyes compared to the fellow and normal control eyes. A subconjunctival injection of APO daily for 11 days at concentrations ranged from 0.025 to 2.5 ng/µl inhibited form deprivation myopia in a concentration-dependent manner. By contrast, the APO treatment ranged from 0.025 to 250 ng/µl did not effectively inhibit the defocus-induced myopia and the associated axial elongation. CONCLUSIONS: DA signaling may play a more critical role in form deprivation myopia than in defocus-induced myopia, raising a question whether the mechanisms of DA signaling are different under these two types of experimental myopia. Molecular Vision 2011-10-31 /pmc/articles/PMC3224832/ /pubmed/22128230 Text en Copyright © 2011 Molecular Vision. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dong, Feng
Zhi, Zhina
Pan, Miaozhen
Xie, Ruozhong
Qin, Xiaoyi
Lu, Runxia
Mao, Xinjie
Chen, Jiang-Fan
Willcox, Mark D.P.
Qu, Jia
Zhou, Xiangtian
Inhibition of experimental myopia by a dopamine agonist: different effectiveness between form deprivation and hyperopic defocus in guinea pigs
title Inhibition of experimental myopia by a dopamine agonist: different effectiveness between form deprivation and hyperopic defocus in guinea pigs
title_full Inhibition of experimental myopia by a dopamine agonist: different effectiveness between form deprivation and hyperopic defocus in guinea pigs
title_fullStr Inhibition of experimental myopia by a dopamine agonist: different effectiveness between form deprivation and hyperopic defocus in guinea pigs
title_full_unstemmed Inhibition of experimental myopia by a dopamine agonist: different effectiveness between form deprivation and hyperopic defocus in guinea pigs
title_short Inhibition of experimental myopia by a dopamine agonist: different effectiveness between form deprivation and hyperopic defocus in guinea pigs
title_sort inhibition of experimental myopia by a dopamine agonist: different effectiveness between form deprivation and hyperopic defocus in guinea pigs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3224832/
https://www.ncbi.nlm.nih.gov/pubmed/22128230
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