The complete genomic sequence of an in vivo low replicating BLV strain

DNA was extracted from lamb lymphocytes that were infected in vivo with a BLV strain after inoculation with the peripheral blood mononuclear cells from a persistently sero-indeterminate, low viral load, BLV-infected Holstein cow (No. 41) from Argentina. The DNA was PCR amplified with a series of ove...

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Autores principales: Dube, Syamalima, Abbott, Lynn, Dube, Dipak K, Dolcini, Guillermina, Gutierrez, Silvina, Ceriani, Carolina, Juliarena, Marcela, Ferrer, Jorge, Perzova, Raisa, Poiesz, Bernard J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3224937/
https://www.ncbi.nlm.nih.gov/pubmed/19650931
http://dx.doi.org/10.1186/1743-422X-6-120
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author Dube, Syamalima
Abbott, Lynn
Dube, Dipak K
Dolcini, Guillermina
Gutierrez, Silvina
Ceriani, Carolina
Juliarena, Marcela
Ferrer, Jorge
Perzova, Raisa
Poiesz, Bernard J
author_facet Dube, Syamalima
Abbott, Lynn
Dube, Dipak K
Dolcini, Guillermina
Gutierrez, Silvina
Ceriani, Carolina
Juliarena, Marcela
Ferrer, Jorge
Perzova, Raisa
Poiesz, Bernard J
author_sort Dube, Syamalima
collection PubMed
description DNA was extracted from lamb lymphocytes that were infected in vivo with a BLV strain after inoculation with the peripheral blood mononuclear cells from a persistently sero-indeterminate, low viral load, BLV-infected Holstein cow (No. 41) from Argentina. The DNA was PCR amplified with a series of overlapping primers encompassing the entire BLV proviral DNA. The amplified BLV ARG 41 DNA was cloned, sequenced, and compared phylogenetically to other BLV sequences including an in vivo high replicating strain (BLV ARG 38) from the same herd in Argentina. Characterization of BLV ARG 41's deduced proteins and its relationship to other members of the PTLV/BLV genus of retroviruses are discussed.
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spelling pubmed-32249372011-11-29 The complete genomic sequence of an in vivo low replicating BLV strain Dube, Syamalima Abbott, Lynn Dube, Dipak K Dolcini, Guillermina Gutierrez, Silvina Ceriani, Carolina Juliarena, Marcela Ferrer, Jorge Perzova, Raisa Poiesz, Bernard J Virol J Research DNA was extracted from lamb lymphocytes that were infected in vivo with a BLV strain after inoculation with the peripheral blood mononuclear cells from a persistently sero-indeterminate, low viral load, BLV-infected Holstein cow (No. 41) from Argentina. The DNA was PCR amplified with a series of overlapping primers encompassing the entire BLV proviral DNA. The amplified BLV ARG 41 DNA was cloned, sequenced, and compared phylogenetically to other BLV sequences including an in vivo high replicating strain (BLV ARG 38) from the same herd in Argentina. Characterization of BLV ARG 41's deduced proteins and its relationship to other members of the PTLV/BLV genus of retroviruses are discussed. BioMed Central 2009-08-03 /pmc/articles/PMC3224937/ /pubmed/19650931 http://dx.doi.org/10.1186/1743-422X-6-120 Text en Copyright ©2009 Dube et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Dube, Syamalima
Abbott, Lynn
Dube, Dipak K
Dolcini, Guillermina
Gutierrez, Silvina
Ceriani, Carolina
Juliarena, Marcela
Ferrer, Jorge
Perzova, Raisa
Poiesz, Bernard J
The complete genomic sequence of an in vivo low replicating BLV strain
title The complete genomic sequence of an in vivo low replicating BLV strain
title_full The complete genomic sequence of an in vivo low replicating BLV strain
title_fullStr The complete genomic sequence of an in vivo low replicating BLV strain
title_full_unstemmed The complete genomic sequence of an in vivo low replicating BLV strain
title_short The complete genomic sequence of an in vivo low replicating BLV strain
title_sort complete genomic sequence of an in vivo low replicating blv strain
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3224937/
https://www.ncbi.nlm.nih.gov/pubmed/19650931
http://dx.doi.org/10.1186/1743-422X-6-120
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