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Microtubules support a disk-like septin arrangement at the plasma membrane of mammalian cells

Septin family proteins oligomerize through guanosine 5′-triphosphate–binding domains into core heteromers, which in turn polymerize at the cleavage furrow of dividing fungal and animal cells. Septin assemblies during the interphase of animal cells remain poorly defined and are the topic of this repo...

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Autores principales: Sellin, Mikael E., Holmfeldt, Per, Stenmark, Sonja, Gullberg, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3226477/
https://www.ncbi.nlm.nih.gov/pubmed/21998205
http://dx.doi.org/10.1091/mbc.E11-09-0754
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author Sellin, Mikael E.
Holmfeldt, Per
Stenmark, Sonja
Gullberg, Martin
author_facet Sellin, Mikael E.
Holmfeldt, Per
Stenmark, Sonja
Gullberg, Martin
author_sort Sellin, Mikael E.
collection PubMed
description Septin family proteins oligomerize through guanosine 5′-triphosphate–binding domains into core heteromers, which in turn polymerize at the cleavage furrow of dividing fungal and animal cells. Septin assemblies during the interphase of animal cells remain poorly defined and are the topic of this report. In this study, we developed protocols for visualization of authentic higher-order assemblies using tagged septins to effectively replace the endogenous gene product within septin core heteromers in human cells. Our analysis revealed that septins assemble into microtubule-supported, disk-like structures at the plasma membrane. In the absence of cell substrate adhesion, this is the predominant higher-order arrangement in interphase cells and each of the seven to eight septin family members expressed by the two analyzed cell types appears equally represented. However, studies of myeloid and lymphoid cell model systems revealed cell type–specific alterations of higher-order septin arrangements in response to substrate adhesion. Live-cell observations suggested that all higher-order septin assemblies are mutually exclusive with plasma membrane regions undergoing remodeling. The combined data point to a mechanism by which densely arranged cortical microtubules, which are typical for nonadhered spherical cells, support plasma membrane–bound, disk-like septin assemblies.
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spelling pubmed-32264772012-02-16 Microtubules support a disk-like septin arrangement at the plasma membrane of mammalian cells Sellin, Mikael E. Holmfeldt, Per Stenmark, Sonja Gullberg, Martin Mol Biol Cell Articles Septin family proteins oligomerize through guanosine 5′-triphosphate–binding domains into core heteromers, which in turn polymerize at the cleavage furrow of dividing fungal and animal cells. Septin assemblies during the interphase of animal cells remain poorly defined and are the topic of this report. In this study, we developed protocols for visualization of authentic higher-order assemblies using tagged septins to effectively replace the endogenous gene product within septin core heteromers in human cells. Our analysis revealed that septins assemble into microtubule-supported, disk-like structures at the plasma membrane. In the absence of cell substrate adhesion, this is the predominant higher-order arrangement in interphase cells and each of the seven to eight septin family members expressed by the two analyzed cell types appears equally represented. However, studies of myeloid and lymphoid cell model systems revealed cell type–specific alterations of higher-order septin arrangements in response to substrate adhesion. Live-cell observations suggested that all higher-order septin assemblies are mutually exclusive with plasma membrane regions undergoing remodeling. The combined data point to a mechanism by which densely arranged cortical microtubules, which are typical for nonadhered spherical cells, support plasma membrane–bound, disk-like septin assemblies. The American Society for Cell Biology 2011-12-01 /pmc/articles/PMC3226477/ /pubmed/21998205 http://dx.doi.org/10.1091/mbc.E11-09-0754 Text en © 2011 Sellin et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Sellin, Mikael E.
Holmfeldt, Per
Stenmark, Sonja
Gullberg, Martin
Microtubules support a disk-like septin arrangement at the plasma membrane of mammalian cells
title Microtubules support a disk-like septin arrangement at the plasma membrane of mammalian cells
title_full Microtubules support a disk-like septin arrangement at the plasma membrane of mammalian cells
title_fullStr Microtubules support a disk-like septin arrangement at the plasma membrane of mammalian cells
title_full_unstemmed Microtubules support a disk-like septin arrangement at the plasma membrane of mammalian cells
title_short Microtubules support a disk-like septin arrangement at the plasma membrane of mammalian cells
title_sort microtubules support a disk-like septin arrangement at the plasma membrane of mammalian cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3226477/
https://www.ncbi.nlm.nih.gov/pubmed/21998205
http://dx.doi.org/10.1091/mbc.E11-09-0754
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