Cargando…

Analysis of the reactivity of indirect immunofluorescence in patients with pemphigus foliaceus and pemphigus vulgaris using rat bladder epithelium as a substrate

OBJECTIVES: To evaluate the reactivity of indirect immunofluorescence using rat bladder epithelium as a substrate in patients with pemphigus foliaceus and pemphigus vulgaris from the Department of Dermatology, University of São Paulo Medical School, Brazil. METHODS: Thirty-two patients (8 male and 2...

Descripción completa

Detalles Bibliográficos
Autores principales: Ortolan, Damaris G., Souza, Danielle P. G., Aoki, Valéria, Santi, Claudia G., Gabbi, Tatiana V. B., Ichimura, Ligia M. F., Maruta, Celina W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3226594/
https://www.ncbi.nlm.nih.gov/pubmed/22189724
http://dx.doi.org/10.1590/S1807-59322011001200004
_version_ 1782217649231495168
author Ortolan, Damaris G.
Souza, Danielle P. G.
Aoki, Valéria
Santi, Claudia G.
Gabbi, Tatiana V. B.
Ichimura, Ligia M. F.
Maruta, Celina W.
author_facet Ortolan, Damaris G.
Souza, Danielle P. G.
Aoki, Valéria
Santi, Claudia G.
Gabbi, Tatiana V. B.
Ichimura, Ligia M. F.
Maruta, Celina W.
author_sort Ortolan, Damaris G.
collection PubMed
description OBJECTIVES: To evaluate the reactivity of indirect immunofluorescence using rat bladder epithelium as a substrate in patients with pemphigus foliaceus and pemphigus vulgaris from the Department of Dermatology, University of São Paulo Medical School, Brazil. METHODS: Thirty-two patients (8 male and 24 female) from the Department of Dermatology, University of São Paulo Medical School, were selected. Three had mucosal pemphigus vulgaris, 20 had mucocutaneous pemphigus vulgaris, and 9 had pemphigus foliaceus. Patients' sera were tested by indirect immunofluorescence performed on human foreskin and rat bladder epithelium and by ELISA assays utilizing baculovirus-expressed recombinant desmoglein 3 and desmoglein 1. RESULTS: No patients with mucosal pemphigus vulgaris, 5 of 20 patients with mucocutaneous pemphigus vulgaris (25%) and 4 of 9 patients with pemphigus foliaceus (44%) had positive indirect immunofluorescence using rat bladder epithelium as a substrate. CONCLUSION: Indirect immunofluorescence using rat bladder epithelium as a substrate is recommended whenever a diagnosis of paraneoplastic pemphigus is considered. The identification of a subset of pemphigus foliaceus and pemphigus vulgaris patients that recognizes desmoplakins by this laboratory tool is critical to avoid the misdiagnosis of paraneoplastic pemphigus.
format Online
Article
Text
id pubmed-3226594
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
record_format MEDLINE/PubMed
spelling pubmed-32265942011-12-02 Analysis of the reactivity of indirect immunofluorescence in patients with pemphigus foliaceus and pemphigus vulgaris using rat bladder epithelium as a substrate Ortolan, Damaris G. Souza, Danielle P. G. Aoki, Valéria Santi, Claudia G. Gabbi, Tatiana V. B. Ichimura, Ligia M. F. Maruta, Celina W. Clinics (Sao Paulo) Clinical Science OBJECTIVES: To evaluate the reactivity of indirect immunofluorescence using rat bladder epithelium as a substrate in patients with pemphigus foliaceus and pemphigus vulgaris from the Department of Dermatology, University of São Paulo Medical School, Brazil. METHODS: Thirty-two patients (8 male and 24 female) from the Department of Dermatology, University of São Paulo Medical School, were selected. Three had mucosal pemphigus vulgaris, 20 had mucocutaneous pemphigus vulgaris, and 9 had pemphigus foliaceus. Patients' sera were tested by indirect immunofluorescence performed on human foreskin and rat bladder epithelium and by ELISA assays utilizing baculovirus-expressed recombinant desmoglein 3 and desmoglein 1. RESULTS: No patients with mucosal pemphigus vulgaris, 5 of 20 patients with mucocutaneous pemphigus vulgaris (25%) and 4 of 9 patients with pemphigus foliaceus (44%) had positive indirect immunofluorescence using rat bladder epithelium as a substrate. CONCLUSION: Indirect immunofluorescence using rat bladder epithelium as a substrate is recommended whenever a diagnosis of paraneoplastic pemphigus is considered. The identification of a subset of pemphigus foliaceus and pemphigus vulgaris patients that recognizes desmoplakins by this laboratory tool is critical to avoid the misdiagnosis of paraneoplastic pemphigus. Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo 2011-12 /pmc/articles/PMC3226594/ /pubmed/22189724 http://dx.doi.org/10.1590/S1807-59322011001200004 Text en Copyright © 2011 Hospital das Clínicas da FMUSP http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Clinical Science
Ortolan, Damaris G.
Souza, Danielle P. G.
Aoki, Valéria
Santi, Claudia G.
Gabbi, Tatiana V. B.
Ichimura, Ligia M. F.
Maruta, Celina W.
Analysis of the reactivity of indirect immunofluorescence in patients with pemphigus foliaceus and pemphigus vulgaris using rat bladder epithelium as a substrate
title Analysis of the reactivity of indirect immunofluorescence in patients with pemphigus foliaceus and pemphigus vulgaris using rat bladder epithelium as a substrate
title_full Analysis of the reactivity of indirect immunofluorescence in patients with pemphigus foliaceus and pemphigus vulgaris using rat bladder epithelium as a substrate
title_fullStr Analysis of the reactivity of indirect immunofluorescence in patients with pemphigus foliaceus and pemphigus vulgaris using rat bladder epithelium as a substrate
title_full_unstemmed Analysis of the reactivity of indirect immunofluorescence in patients with pemphigus foliaceus and pemphigus vulgaris using rat bladder epithelium as a substrate
title_short Analysis of the reactivity of indirect immunofluorescence in patients with pemphigus foliaceus and pemphigus vulgaris using rat bladder epithelium as a substrate
title_sort analysis of the reactivity of indirect immunofluorescence in patients with pemphigus foliaceus and pemphigus vulgaris using rat bladder epithelium as a substrate
topic Clinical Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3226594/
https://www.ncbi.nlm.nih.gov/pubmed/22189724
http://dx.doi.org/10.1590/S1807-59322011001200004
work_keys_str_mv AT ortolandamarisg analysisofthereactivityofindirectimmunofluorescenceinpatientswithpemphigusfoliaceusandpemphigusvulgarisusingratbladderepitheliumasasubstrate
AT souzadaniellepg analysisofthereactivityofindirectimmunofluorescenceinpatientswithpemphigusfoliaceusandpemphigusvulgarisusingratbladderepitheliumasasubstrate
AT aokivaleria analysisofthereactivityofindirectimmunofluorescenceinpatientswithpemphigusfoliaceusandpemphigusvulgarisusingratbladderepitheliumasasubstrate
AT santiclaudiag analysisofthereactivityofindirectimmunofluorescenceinpatientswithpemphigusfoliaceusandpemphigusvulgarisusingratbladderepitheliumasasubstrate
AT gabbitatianavb analysisofthereactivityofindirectimmunofluorescenceinpatientswithpemphigusfoliaceusandpemphigusvulgarisusingratbladderepitheliumasasubstrate
AT ichimuraligiamf analysisofthereactivityofindirectimmunofluorescenceinpatientswithpemphigusfoliaceusandpemphigusvulgarisusingratbladderepitheliumasasubstrate
AT marutacelinaw analysisofthereactivityofindirectimmunofluorescenceinpatientswithpemphigusfoliaceusandpemphigusvulgarisusingratbladderepitheliumasasubstrate